WorksheetsModule 2 Microbio Lec
Total questions: 50
Worksheet time: 38mins
Bacteria normally reproduce by _______
Meiosis
Mitosis
Binary fission
Because one cell gives rise to two progeny cells, bacteria are said to undergo (a)
(a) is the time required for a cell to divide and its population to double. It varies considerably among organisms and with environmental conditions like temperature, pH, etc.
This is the period of little or no cell division so the population remains temporarily unchanged.
Lag phase
Log phase
Stationary phase
Death phase
No apparent cell division occurs but the cells maybe growing in volume or size, synthesizing enzymes, proteins etc. and increasing in metabolic activity in preparation for the next phase.
Lag phase
Log phase
Stationary phase
Death phase
The cells begin to divide and enter a period of growth or logarithmic increase. Cellular reproduction is most active during this period and generation time reaches a constant minimum.
Lag phase
Log phase
Stationary phase
Death phase
Exponential growth cannot be continued forever in a batch because of the following reasons:
Exhaustion of nutrients
Accumulation of inhibitory metabolites
Exhaustion of space
Lack of biological space
Growth slows down due to nutrient depletion and formation of toxic products.
Log phase
Lag phase
Stationary phase
Death phase
In this phase, the number of new cells produced balances the number of cells that die. This results in a steady state.
Log phase
Lag phase
Stationary phase
Death phase
It is characterized by an exponential decline or decrease in the number of viable bacteria. For a microbial cell, death means the irreversible loss of the ability to reproduce.
Log phase
Lag phase
Decline phase
Death phase
(a) is the process of propagating microorganisms by providing the proper environmental conditions.
Factors that must be controlled during growth includes
nutrients
size
temperature
aeration
salt concentration
It is characterized by substrate phosphorylation, an enzymatic process in which a pyrophosphate bond is donated directly to ADP by a phosphorylated metabolic intermediates like glucose, lactose or arginine.The formation of ATP in fermentation is not coupled by the transfer of electrons. It is characterized by substrate phosphorylation, an enzymatic process in which a pyrophosphate bond is donated directly to ADP by a phosphorylated metabolic intermediates like glucose, lactose or arginine. The formation of ATP is not coupled by the transfer of electrons.
(a)
It is a process that involves the chemical reduction of an oxidant (electron acceptor) through a specific series of electron carriers in the membrane.
(a)
It is process that involves chemical reduction of an oxidant via a specific series of electron carriers similar in respiration.
(a)
(a) is structural backbone of living matter. It is required for microbial growth and it can be obtained from organic materials like proteins, carbohydrates and lipids.
_____ is used in forming the amino group of the amino acids of proteins.
Nitrogen
Carbon
Sulfur
Phosphorus
It can be obtained from nitrate, nitrite, ammonium, amine and amino acids.
Nitrogen
Sulfur
Carbon
Mineral sources
_____ is a component of many organic cell substance forming part of several coenzymes and found in the cysteinyl and methionyl side chains of proteins.
Sulfur
Nitrogen
Carbon
Phosphorus
_______ in the form of phosphate is required as a component of ATP, nucleic acids and coenzymes like NAD, NADP and flavins.
Phosphorus
Oxygen
Nitrogen
Sodium
_____ is found in chlorophyll molecules
Mg+2
Fe+2
Mn+2
Na+
____ is part of the coenzyme of the cytochromes and peroxidases
Fe+2
Mg+2
K+
Ca+2
____ is/are essential for the function and integrity of ribosomes
Mg+2 and K+
Mg+2 and Na
Ca+2
SO4
_____ is a required constituent of gram-positive cell walls
Ca+2
Mg+2
K+
Zn+2
Nutritional type of Prokaryotic cell; Energy source of photoautotrophs is ___
Light source
NOTA
Inorganic compounds
Organic compounds
Nutritional Types of Prokaryotes
Energy source is inorganic compounds while carbon source is carbon dioxide
Photoautotrophs
Photoheterotrophs
Chemoautotrophs or
Lithotrophs
Chemoautotrophs or
Lithotrophs
Nutritional Types of Prokaryotes;
Energy and carbon source are organic compounds
Photoautotrophs
Photoheterotrophs
Chemoautotrophs or
Lithotrophs
Chemoheterotrophs or
Heterotrophs
microorganisms that live in a pH of 3.0 or acidic.
Acidophiles
Basophiles
Neutralophiles
Acidosis
microorganisms that live in a pH of 10.5 or basic.
Acidophiles
Basophiles or Alkaliphiles
Neutralophiles
microorganisms that live in a pH range of 6.0 to 8.0
Acidophiles
Neutralophiles
Alkaliphiles
Basophiles
grow best at low temperatures (15C to 20C)
Psychrophiles
Mesophiles
Thermophile
Hyperthermophiles
grow best at 30C to 37C
Psychrophiles
Mesophiles
Thermophiles
Hyperthermophiles
grow best at 50C to 60C
Psychrophiles
Mesophiles
Thermophiles
Hyperthermophiles
grow well above the temperature of boiling water
Psychrophiles
Mesophiles
Thermophiles
Hyperthermophiles
Beyond the effects on growth rate, extremes of temperature can also inhibit the growth by _____ or kill microorganisms.
sterilization by autoclaving
preservation in a bioref
NOTA
All of the choices
Beyond the effects on growth rate, extremes of temperature can also inhibit the growth or kill microorganisms by ______
NOTA
All of the choice
preservation in a bioref
sterilization by autoclaving
The use of oxygen generates two toxic substances:
hydrogen peroxide and superoxide radical.
water and superoxide radical
sodium chloride and superoxide radical
NOTA
_______ catalyzes the reaction of the superoxide radical with hydrogen forming hydrogen peroxide and oxygen.
superoxide dismuthase
catalase
All of the choices
Either of the two choices
________ catalyzes the breakdown of hydrogen peroxide into water and oxygen.
superoxide dismuthase
catalase
Either of the two choices
NOTA
require 21% oxygen because they possess both SOD and catalase enzymes.
Aerobes or Obligate Aerobes
Microaerophilic organisms
Obligate or Strict Anaerobes
Facultative Anaerobes
Capneic
require 2 to 105 of oxygen.
Aerobes or Obligate Aerobes
Microaerophilic organisms
Facultative Anaerobes
Capneic
survive in the absence of oxygen because they lack both SOD and catalase enzymes.
Obligate or Strict Anaerobes
Microaerophilic organisms
Facultative Anaerobes
Aerotolerant Anaerobes
can survive in the presence or absence of oxygen because they possess either SOD or catalase.
Facultative Anaerobes
Aerotolerant Anaerobes
Obligate Anaerobes
Obligate Aerobes
can grow in the presence of oxygen but grow best in its absence.
Aerotolerant Anaerobes
Facultative Anaerobes
Obligate Anaerobes
Obligate Aerobes
requires 5 to 10% of carbon dioxide, instead of oxygen.
Capneic
Microaerophilic organism
Aerobes
Facultative Anaerobes
______ are organisms requiring high salt concentration.
Halophiles
Osmophiles
Electrophiles
Nucleophiles
____ are organisms requiring high osmotic pressure.
Osmophiles
Halophiles
Nucleophiles
Electrophiles
Sources of Metabolic Energy
Fermentation
Respiration
Photosynthesis
All of the choices
If the number of cells in 0 hours is 2 then, in 20 minutes is 4. What is the number of cells in 40 minutes?
Two of the choices
8
4
2
Osmotic conditions: microbes tolerating NaCl are ____
Halotolerant
Nonhalophiles
Extreme halophiles
Acidophiles
