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Module 2 Microbio Lec

Total questions: 50

Worksheet time: 38mins

Name
Class
Date
1.

Bacteria normally reproduce by _______

a)

Meiosis

b)

Mitosis

c)

Binary fission

2.

Because one cell gives rise to two progeny cells, bacteria are said to undergo (a)  

3.

(a)   is the time required for a cell to divide and its population to double. It varies considerably among organisms and with environmental conditions like temperature, pH, etc.

4.

This is the period of little or no cell division so the population remains temporarily unchanged.

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Death phase

5.

No apparent cell division occurs but the cells maybe growing in volume or size, synthesizing enzymes, proteins etc. and increasing in metabolic activity in preparation for the next phase.

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Death phase

6.

The cells begin to divide and enter a period of growth or logarithmic increase. Cellular reproduction is most active during this period and generation time reaches a constant minimum.

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Death phase

7.

Exponential growth cannot be continued forever in a batch because of the following reasons:

a)

Exhaustion of nutrients

b)

Accumulation of inhibitory metabolites

c)

Exhaustion of space

d)

Lack of biological space

8.

Growth slows down due to nutrient depletion and formation of toxic products.

a)

Log phase

b)

Lag phase

c)

Stationary phase

d)

Death phase

9.

In this phase, the number of new cells produced balances the number of cells that die. This results in a steady state.

a)

Log phase

b)

Lag phase

c)

Stationary phase

d)

Death phase

10.

It is characterized by an exponential decline or decrease in the number of viable bacteria. For a microbial cell, death means the irreversible loss of the ability to reproduce.

a)

Log phase

b)

Lag phase

c)

Decline phase

d)

Death phase

11.

(a)   is the process of propagating microorganisms by providing the proper environmental conditions.

12.

Factors that must be controlled during growth includes

a)

nutrients

b)

size

c)

temperature

d)

aeration

e)

salt concentration

13.

It is characterized by substrate phosphorylation, an enzymatic process in which a pyrophosphate bond is donated directly to ADP by a phosphorylated metabolic intermediates like glucose, lactose or arginine.The formation of ATP in fermentation is not coupled by the transfer of electrons. It is characterized by substrate phosphorylation, an enzymatic process in which a pyrophosphate bond is donated directly to ADP by a phosphorylated metabolic intermediates like glucose, lactose or arginine. The formation of ATP is not coupled by the transfer of electrons.

(a)  

14.

It is a process that involves the chemical reduction of an oxidant (electron acceptor) through a specific series of electron carriers in the membrane.

(a)  

15.

It is process that involves chemical reduction of an oxidant via a specific series of electron carriers similar in respiration.

(a)  

16.

(a)   is structural backbone of living matter. It is required for microbial growth and it can be obtained from organic materials like proteins, carbohydrates and lipids.

17.

_____ is used in forming the amino group of the amino acids of proteins.

a)

Nitrogen

b)

Carbon

c)

Sulfur

d)

Phosphorus

18.

It can be obtained from nitrate, nitrite, ammonium, amine and amino acids.

a)

Nitrogen

b)

Sulfur

c)

Carbon

d)

Mineral sources

19.

_____ is a component of many organic cell substance forming part of several coenzymes and found in the cysteinyl and methionyl side chains of proteins.

a)

Sulfur

b)

Nitrogen

c)

Carbon

d)

Phosphorus

20.

_______ in the form of phosphate is required as a component of ATP, nucleic acids and coenzymes like NAD, NADP and flavins.

a)

Phosphorus

b)

Oxygen

c)

Nitrogen

d)

Sodium

21.

_____ is found in chlorophyll molecules

a)

Mg+2

b)

Fe+2

c)

Mn+2

d)

Na+

22.

____ is part of the coenzyme of the cytochromes and peroxidases

a)

Fe+2

b)

Mg+2

c)

K+

d)

Ca+2

23.

____ is/are essential for the function and integrity of ribosomes

a)

Mg+2 and K+

b)

Mg+2 and Na

c)

Ca+2

d)

SO4

24.

_____ is a required constituent of gram-positive cell walls

a)

Ca+2

b)

Mg+2

c)

K+

d)

Zn+2

25.

Nutritional type of Prokaryotic cell; Energy source of photoautotrophs is ___

a)

Light source

b)

NOTA

c)

Inorganic compounds

d)

Organic compounds

26.

Nutritional Types of Prokaryotes

Energy source is inorganic compounds while carbon source is carbon dioxide

a)

Photoautotrophs

b)

Photoheterotrophs

c)

Chemoautotrophs or

Lithotrophs

d)

Chemoautotrophs or

Lithotrophs

27.

Nutritional Types of Prokaryotes;

Energy and carbon source are organic compounds

a)

Photoautotrophs

b)

Photoheterotrophs

c)

Chemoautotrophs or

Lithotrophs

d)

Chemoheterotrophs or

Heterotrophs

28.

microorganisms that live in a pH of 3.0 or acidic.

a)

Acidophiles

b)

Basophiles

c)

Neutralophiles

d)

Acidosis

29.

microorganisms that live in a pH of 10.5 or basic.

a)

Acidophiles

b)

Basophiles or Alkaliphiles

c)

Neutralophiles

30.

microorganisms that live in a pH range of 6.0 to 8.0

a)

Acidophiles

b)

Neutralophiles

c)

Alkaliphiles

d)

Basophiles

31.

grow best at low temperatures (15C to 20C)

a)

Psychrophiles

b)

Mesophiles

c)

Thermophile

d)

Hyperthermophiles

32.

grow best at 30C to 37C

a)

Psychrophiles

b)

Mesophiles

c)

Thermophiles

d)

Hyperthermophiles

33.

grow best at 50C to 60C

a)

Psychrophiles

b)

Mesophiles

c)

Thermophiles

d)

Hyperthermophiles

34.

grow well above the temperature of boiling water

a)

Psychrophiles

b)

Mesophiles

c)

Thermophiles

d)

Hyperthermophiles

35.

Beyond the effects on growth rate, extremes of temperature can also inhibit the growth by _____ or kill microorganisms.

a)

sterilization by autoclaving

b)

preservation in a bioref

c)

NOTA

d)

All of the choices

36.

Beyond the effects on growth rate, extremes of temperature can also inhibit the growth or kill microorganisms by ______

a)

NOTA

b)

All of the choice

c)

preservation in a bioref

d)

sterilization by autoclaving

37.

The use of oxygen generates two toxic substances:

a)

hydrogen peroxide and superoxide radical.

b)

water and superoxide radical

c)

sodium chloride and superoxide radical

d)

NOTA

38.

_______ catalyzes the reaction of the superoxide radical with hydrogen forming hydrogen peroxide and oxygen.

a)

superoxide dismuthase

b)

catalase

c)

All of the choices

d)

Either of the two choices

39.

________ catalyzes the breakdown of hydrogen peroxide into water and oxygen.

a)

superoxide dismuthase

b)

catalase

c)

Either of the two choices

d)

NOTA

40.

require 21% oxygen because they possess both SOD and catalase enzymes.

a)

Aerobes or Obligate Aerobes

b)

Microaerophilic organisms

c)

Obligate or Strict Anaerobes

d)

Facultative Anaerobes

e)

Capneic

41.

require 2 to 105 of oxygen.

a)

Aerobes or Obligate Aerobes

b)

Microaerophilic organisms

c)

Facultative Anaerobes

d)

Capneic

42.

survive in the absence of oxygen because they lack both SOD and catalase enzymes.

a)

Obligate or Strict Anaerobes

b)

Microaerophilic organisms

c)

Facultative Anaerobes

d)

Aerotolerant Anaerobes

43.

can survive in the presence or absence of oxygen because they possess either SOD or catalase.

a)

Facultative Anaerobes

b)

Aerotolerant Anaerobes

c)

Obligate Anaerobes

d)

Obligate Aerobes

44.

can grow in the presence of oxygen but grow best in its absence.

a)

Aerotolerant Anaerobes

b)

Facultative Anaerobes

c)

Obligate Anaerobes

d)

Obligate Aerobes

45.

requires 5 to 10% of carbon dioxide, instead of oxygen.

a)

Capneic

b)

Microaerophilic organism

c)

Aerobes

d)

Facultative Anaerobes

46.

______ are organisms requiring high salt concentration.

a)

Halophiles

b)

Osmophiles

c)

Electrophiles

d)

Nucleophiles

47.

____ are organisms requiring high osmotic pressure.

a)

Osmophiles

b)

Halophiles

c)

Nucleophiles

d)

Electrophiles

48.

Sources of Metabolic Energy

a)

Fermentation

b)

Respiration

c)

Photosynthesis

d)

All of the choices

49.

If the number of cells in 0 hours is 2 then, in 20 minutes is 4. What is the number of cells in 40 minutes?

a)

Two of the choices

b)

8

c)

4

d)

2

50.

Osmotic conditions: microbes tolerating NaCl are ____

a)

Halotolerant

b)

Nonhalophiles

c)

Extreme halophiles

d)

Acidophiles