Font size
WorksheetsREVIEWER
Total questions: 115
Worksheet time: 2hrs 55mins
Is the use of microscopes to view objects that cannot normally be viewed by your naked, unaided eye.
(a)
Allow scientists to examine specimens in more detail, whether the specimen is a biological sample, a plant, or a crystal structure.
(a)
When using your microscope, the objective lens is not lowered to adjust the focus. However, be careful not to let the lens touch the slide your looking at, as this can damage the lens. Furthermore, dirty lenses are not difficult to clean
true
false
Improper handling is a common cause of many problems that occur with microscopes. When carrying a microscope, hold it by the base and the metal support arm. The stage on a microscope is the flat plate where the slides are placed for observation. Avoid picking your microscope up by the stage or the eyepiece holder, as this can cause misalignment.
true
false
Microscopes should always be sold with dust covers. Whether transporting or storing your instrument, make the most of the microscope bag and remember to keep your microscope covered when not in use. The microscope's eye tubes do not need to be dust free. If the eyepieces need to be removed, cover the tubes with caps and store them with the microscope.
true
false
Microscopes lenses are delicate. Treat them carefully to avoid any scratches. Avoid using an aspirator to remove dust. Moisten special lens paper with distilled water or appropriate cleaning solution. Rubbing gently in any direction can remove any sticky residue. Never use anything abrasive in microscope lenses.
true
false
Oil immersion is a technique used to increase the resolving power of a microscope. Both the objective lens and sample are immersed in a transparent oil of high refractive index so that high magnifications can be achieved while still maintaining good resolution. It is essential to ensure careful cleaning takes place immediately after using immersion oil and do not use damaging solvents.
true
false
After using your microscope, turn off the illuminator and wait for it to cool down before putting it away. Allowing the bulb to cool will extend its life and avoid the unnecessary cost of expensive replacements. Similarly, if used constantly on full power, the bulb will overheat and blow. Remember too, to turn the illuminator off when not in use.
true
false
Your microscope should be sold with a user's manual and specialist spanners as required. You can also not refer to the manual when making adjustments to the microscope and use supplied spanners. Use force, but never use inappropriate tools or overtighten when making adjustments to your microscope, as thill result in equipment damage.
true
false
An annual maintenance check of microscopes is always a good idea. Moving parts should be cleaned and lubricated. Similarly, inspect the power cords and plugs for safety.
true
false
The company where microscope was bought can be contacted for a range of products to help keep your laboratory microscopy equipment in tip top condition.
true
false
A good, unaided eye can separate objects that are about
0.15-0.20 mm apart
0.10-0.15 mm apart
0.5-0.10 mm apart
It cannot be easily recognized unless you will manipulate something to see finer details of such object of study.
(a)
means of viewing
(a)
of minute size
(a)
Contains the ocular lens which you will be looking through to see the magnified specimens with magnification raging from 5X to 30X, but 10X or 15X is the most common in use. The ocular lens provides a re-magnified image to see when light enters through the objective lens.
Eyepiece tube
Eyepiece lens
Microscope Illuminator
It is a light source which can come in a form of a built-in, low voltage illuminator light, or a mirror that reflects an external light source like sunlight.
Microscope Illuminator
Microscope Arm
Microscope Base
It connects the eyepiece and ocular lens to the objective lenses.
Eyepiece Tube
Eyepiece Lens
Microscope Base
It connects the eyepiece tube to the base where you should hold when carrying the microscope.
Microscope Tube
Microscope Illuminator
Microscope Arm
It provides stability and support for the microscope in its upright position. Typically, it holds the source of light or illuminator.
Microscope Base
Microscope Arm
Microscope Nosepiece
It serves as the platform for slides which hold the specimen in place through stage clip on either side. Some has mechanical stage with adjustment knobs that allow movement of slides to achieve more precise positioning.
Microscope Base
The Stage and Stage Clips
Rack Stop
It contains the objective lenses. You can rotate this part in switching objective lenses and adjust the magnification power.
Objective Lenses
Microscope Nosepiece
Rack Stop
Are combining with the eyepiece lens to increase magnification levels. Objective lenses are the lenses that protrude downward over the specimen.
Rack Stop
Condenser Lens and Diaphragm
Objective Lenses
It prevents users from moving the objectives too close to the slide.
Rack Stop
Control Focus Knobs
Coarse adjustment Knob
Turning the knobs adjusts the distance between the stage and the lens.
Control Focus Knob
Coarse Adjustment Knob
Fine Adjustment Knob
It is then turned to bring the specimen into sharp focus.
Condenser Lens and Diaphragm
Fine Adjustment Knob
Coarse Adjustment Knob
It concentrates the light on the specimen.
Condenser Lens
Objective Lenses
Diaphragm
It is used to bring the specimen into initial focus -- visible but not sharp.
Fine Adjustment Knob
Control Focus Knob
Coarse Adjustment Knob
With a small movable lever is adjusted that regulates the entry of light.
Diaphragm
Condenser Lens
Objective Lens
1. Place sample seed of sesame on a clean glass slide at the center and cover it.
Dry mount slide
Wet mount slide
1. Place a drop of water at the center of the slide.
2. Using tweezer, place pollen of gumamela flower or any flower with pollen in the middle of the drop.
3. While holding the cover slip upright and by the edges, carefully place one edge of the cover slip next to the water.
4. Set one edge against the slide and lower it until it contacts the liquid. Slowly lower the upper edge of the cover slip onto the water.
5. Absorbent towel can be placed at the edge of the cover slip to draw out some of the water, further flattening the wet mount slide
Dry mount slide
Wet mount slide
First step in Staining a Slide
If the stain has covered the area containing the specimen, you are done. (The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it covers.)
Place one drop of methylene blue stain or iodine solution on one edge of the cover slip and the flat edge of
a piece of paper towel on the other edge of the coverslip. (The paper towel will draw the water out from under the coverslip and the cohesion of water will draw the stain under the coverslip.)
The slide can now be placed on the microscopic stage.
Click this link and be infused with some information about these
Wipe off the excess stain with a paper towel so it will not mess up with objective lenses.
Second step in Staining a Slide
Place one drop of methylene blue stain or iodine solution on one edge of the cover slip and the flat edge of
a piece of paper towel on the other edge of the coverslip. (The paper towel will draw the water out from under the coverslip and the cohesion of water will draw the stain under the coverslip.)
The slide can now be placed on the microscopic stage.
Click this link and be infused with some information about these
If the stain has covered the area containing the specimen, you are done. (The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it covers.)
Wipe off the excess stain with a paper towel so it will not mess up with objective lenses.
Third step in staining a Slide
Place one drop of methylene blue stain or iodine solution on one edge of the cover slip and the flat edge of
a piece of paper towel on the other edge of the coverslip. (The paper towel will draw the water out from under the coverslip and the cohesion of water will draw the stain under the coverslip.)
If the stain has covered the area containing the specimen, you are done. (The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it covers.)
The slide can now be placed on the microscopic stage.
Wipe off the excess stain with a paper towel so it will not mess up with objective lenses.
Last step in Staining a Slide
If the stain has covered the area containing the specimen, you are done. (The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it covers.)
Place one drop of methylene blue stain or iodine solution on one edge of the cover slip and the flat edge of
a piece of paper towel on the other edge of the coverslip. (The paper towel will draw the water out from under the coverslip and the cohesion of water will draw the stain under the coverslip.)
Wipe off the excess stain with a paper towel so it will not mess up with objective lenses.
The slide can now be placed on the microscopic stage.
First five steps on how to focus image under microscope
-Adjust the condenser and light intensity for the greatest amount of light.
-Move the microscope slide around until the sample is in the center of the field of view.
-Manipulate the focus knob and readjust the condenser and light intensity to obtain the clearest image possible.
-Once the image is sharp with LPO, move to HPO and do minor adjustments using fine adjustment knob. (You might need to readjust the sample into focus and/or readjust the condenser and light intensity).
Do not let the objective lens touch the slide!
Both eyes should be open when viewing through the microscope.
-Complete your drawings (image seen under LPO, HPO).
-Place the microscope on a flat, level, firm table free from vibration seeing to it that the arm is towards you while the stage is going away from you. Do not place it in front of a brightly lit window.
-Turn on the light source and adjust the optimum light setting to ensure correct level of brightness by turning or sliding the brightness adjustment knob at the base.
-Turn the revolving nosepiece (turret) so that the lowest power objective lens is clicked into position.
-Place the slide (dry mount, wet mount, prepared mount) on the mechanical stage secured with clips.
-Look through the eyepiece and move the focus knob until the image comes into focus.
Sixth to eighth step on how to focus image under a microscope
-Complete your drawings (image seen under LPO, HPO).
-When finished, lower the stage, click the low power lens into position and remove the slide.
-Wash and dry the slides and keep it on your storage rack or tray.
-Look through the eyepiece and move the focus knob until the image comes into focus.
-Adjust the condenser and light intensity for the greatest amount of light.
-Move the microscope slide around until the sample is in the center of the field of view.
-Adjust the condenser and light intensity for the greatest amount of light.
-Move the microscope slide around until the sample is in the center of the field of view.
-Manipulate the focus knob and readjust the condenser and light intensity to obtain the clearest image possible.
ninth to last step on how to focus image under microscope.
-Once the image is sharp with LPO, move to HPO and do minor adjustments using fine adjustment knob. (You might need to readjust the sample into focus and/or readjust the condenser and light intensity).
-Do not let the objective lens touch the slide!
Both eyes should be open when viewing through the microscope.
-Complete your drawings (image seen under LPO, HPO).
-When finished, lower the stage, click the low power lens into position and remove the slide.
-Wash and dry the slides and keep it on your storage rack or tray.
-Place the microscope on a flat, level, firm table free from vibration seeing to it that the arm is towards you while the stage is going away from you. Do not place it in front of a brightly lit window.
-Turn on the light source and adjust the optimum light setting to ensure correct level of brightness by turning or sliding the brightness adjustment knob at the base.
-Turn the revolving nosepiece (turret) so that the lowest power objective lens is clicked into position.
-Place the slide (dry mount, wet mount, prepared mount) on the mechanical stage secured with clips.
What are the two parameters in microscopy?
(a)
The measure for the ability to tell two points apart. It describes whether two adjoining points can still be perceived as separate.
(a)
Refers to the distance from the cover glass to the nearest point of the objective.
(a)
is the product of Vobjective x Vocular
(a)
Refers to the distance between the upper and lower limits of sharpness in the image formed by an optical system.
(a)
Is the sine of half the angle of the cone of light from each point of the object that can be accepted by the objective multiplied by the index of refraction of the medium in which the object is immersed.
(a)
The actual size of the field of view in mm on the object surface
(a)
This is a number that represents the diameter in mm of the image of the field diaphragm that is formed by the lens in front of it.
(a)
the function of a two-lens system.
(a)
It is found in the eyepiece.
(a)
It is situated in a revolving nose-piece.
(a)
It separates the ocular lens and objective lens.
(a)
It does not describe the quality of the image.
(a)
drawing size / actual size.
(a)
It is one of the main branches of Biology (science of life) that focuses on the systematic and scientific study of plants.
(a)
Are important in balancing nature and in keeping human lives.
(a)
plants the possess (a) , manufacture their own food and generates oxygen.
Has many branches but the two most common subjects are: Botany and Zoology. – A natural science.
(a)
The earth is said to have come into existence 5 million years ago and life on earth came into existence only a billion years after that.
true
false
A British naturalist proposed the theory of biological evolution by natural selection.
(a)
the idea that species change over time, give rise to new species, and share common ancestors.
(a)
What are the 3 key observations of Darwin's concept of natural selection?
(a)
Study structure and function of important biological molecule like proteins and nucleic acid.
(a)
Plant heredity & variation.
(a)
structure, function & life processes of plants
(a)
study of chemical interactions within plant.
(a)
evolutionary relationship among different plant group.
(a)
processes (i.e. photosynthesis & mineral nutrition)
(a)
plants with commercial importance.
(a)
plant cell & tissues
(a)
deals with forest conservation & forest.
(a)
structure, function & life processes of plants
(a)
ornamental plants and fruits & vegetable crops
(a)
a sub discipline of systematics; deals with description, naming & classification of plants.
(a)
plant heredity & variation.
(a)
study of field crops & soils.
(a)
study of interrelationship among plants and between plants & their environment.
(a)
study of mosses & similar plants.
(a)
study of the biology & the evolution of plants in the geologic past.
(a)
(Papaver somniferum L., Papaveraceae), which was first identified by Friedrich Wilhelm Sertürner of Germany 1804 and chemically characterized in 1817 as an alkaloid.
(a)
(Salix spp., Salicaceae), was first isolated by Johannes Buchner in Germany. It was derivatized first (in 1838) by Rafaele Pirea (France) to yield salicylic acid, and later (1899) by the Bayer company, to yield acetylsalicylic acid, or aspirin.
(a)
a remedy is foxglove (Digitalis purpurea), reportedly used by an English housewife to treat dropsy, and then more systematically by the physician William Withering (1741–1799).
(a)
He is known today for his identification of the cellular structure of plants.
(a)
It was the first important work on microscopy, the study of minute objects through a microscope
(a)
Are considered to be the founders of the study of plant anatomy, for they examined in detail the structure and development of many plants.
(a)
He is from Italy that discovered various tissues in stems and roots.
(a)
A Flemish physician and chemist, who was the first to demonstrate that plants do not have the same nutritional needs as animals.
(a)
He discovered accidentally that gases play a role in photosynthesis.
(a)
Plants absorb water as a result of what we now know as
(a)
The Swedish botanist, owed for the present system of naming and classifying plants and animals by using generic name (genus) and a specific name (species).
(a)
US botanist, in 1895 he coined the term "ethnobotany".
(a)
In 1735, Carolus Linnaeus published (a) that organized all plants and animals from the level of kingdoms all the way down to species.
In 1753 publication, (a) , marked the initial use of the nomenclature for all flowering plants and ferns.
In 1866, a German scientist, Ernst Haeckel coined the word
(a)
1st Century BCE. A Greek scholar, Pedanius Dioscorides published 5 volumes of a book entitled “De Materia Medica” in 78 AD, which described more than 600 medicinal plants with their collection, storage and uses.
(a)
Shennong's Classic of Materia Medica or the Shen-nung pen-tsao ching, which provides the earliest treatise of Chinese medicine theory classical sources on Chinese traditional medicine including 365 drugs, most of botanical origin.
(a)
Graeco-Roman medical doctor who summarized the complex body of Graeco-Roman pharmacy and medicine
(a)
He identified aloe, belladonna, colchicum, ergot, hyoscyamus, and opium.
(a)
Claudius Galen's name survived in the pharmaceutical term
(a)
His Classic of Internal Medicine is important in understanding the basic ideas of traditional Chinese herbal science, acupuncture, moxibustion Yin and Yang, the Five Phases of Evolutive.
(a)
He is believed to have introduced the technique of acupuncture.
(a)
German toxicologist first to study psychoactive plants systematically
(a)
Performed classic experiments with pea plants.
(a)
He is often referred to as the "father of phytogeography". He advocated a quantitative approach to phytogeography that has characterized modern plant geography.
(a)
Refers to the process where the members of a population become better suited to some feature of their environment through change in a characteristic that affects their survival or reproduction.
(a)
which means “to fit”
(a)
very dry and often hot. some plants called succulents and plants that have no leaves or small adapt in this environment
(a)
Rain is plentiful, temperature varies from hot in the summer to below freezing in winter. Wildflowers grow on forest floor early in the spring before trees leaf-out and shade the forest floor. Trees have thick bark to protect against cold winters.
(a)
Large evergreen trees, some reaching 300 feet in height, are the dominant plant species. Receives a lot of precipitation, Condensation from coastal fogs also add to the dampness.
(a)
Also known as boreal forests, many trees have needle-like leaves which shape loses less water and sheds snow more easily than broad leaves. Some parts of the taiga have a permanently frozen sub layer of soil called permafrost.
(a)
Underwater leaves and stems are flexible to move with water currents, submerged plants lack strong water transport system (in stems); instead water, nutrients, and dissolved gases are absorbed through the leaves directly from the water
(a)
Has long days during the growing season, sometimes with 24 hours of daylight, and long nights during the winter. Plants are dark in color—some are even red—this helps them absorb solar heat. Some plants are covered with hair which helps keep them warm.
(a)
Water-loving plants, may grow entirely or partly submerged, floating on the water surface, or with their roots anchored to the ground in swamps or beside bodies of water. ex. gabi/taro, rice, water lily, lotus
(a)
chalk-loving plants and acid-escaping plants, those that prefer calcareous or alkaline soils or soils with pH above 7.0. ex. alfalfa, blazing star
(a)
a plant which needs very little water. ex. pineapple, variety of cacti
(a)
air plants and tree dwellers. They grow above ground on another plant but they are not parasitic. They usually need the host only for physical support, deriving their nutrition from the air. ex. family Bromeliaceae including the ornamental bromyliads, and other orchid and fern families, strangler fig.
(a)
chalk-escaping, lime-hating, acidophilous, acid-loving, and acid soil plants. Those that prefer acidic soils or soils having pH levels below 7.0 but do not tolerate alkaline (basic) or calcareous soils. ex. rhododendrons and azaleas
(a)
