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Microbial growth

Total questions: 25

Worksheet time: 13mins

Name
Class
Date
1.

Bacteria make necessary enzymes for growth/reproduction.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

2.

Most binary fission occurs.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

3.

#New cells = #Dying cells

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

4.

No nutrients or space remain for growth.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

5.

Spores are formed or new protective genes are activated.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

6.

Culture media provide microbes with:

a)

CO2

b)

O2

c)

nutrients

d)

agar

7.

Bacteria growing in natural environments form:

a)

colonies

b)

pure cultures

c)

spores

d)

biofilms

8.

Bacteria growing in lab environments form:

a)

colonies

b)

gangs

c)

spores

d)

biofilms

9.

Biofilms are associated with more than 50% of infections.

a)

True

b)

False

10.

The two most common types of media used in the laboratory are:

a)

chemically defined & simple

b)

agar & broth

c)

simple & complex

d)

differential & selective

11.

A single colony:

a)

is grown to provide a pure culture

b)

starts as a single bacterial cell

c)

consists of identical "clones"

d)

all of these

12.

Is H2O2 (hydrogen peroxide) toxic?

a)

No

b)

Yes, it is always toxic

c)

Not if you produce catalase and peroxidase

d)

Not if you produce superoxide dismutase

13.

A medium in which the exact chemical composition varies slightly between batches:

a)

nonselective

b)

differential

c)

complex

d)

simple

14.

Fastidious organisms:

a)

are motile

b)

are anaerobic

c)

require many growth factors

d)

grow slowly

15.

Why is it that organisms that use and/or tolerate oxygen must have superoxide dismutase?

a)

Oxygen free radicals are toxic

b)

To produce the final electron acceptor superoxide from water

c)

To form metabolic water from oxygen and hydrogen ions

d)

Dismutation requires oxygen

16.

Which of the following conditions do you think would present the greatest problem for bacterial survival?

a)

hypotonic solution

b)

low temperature

c)

neutral pH

d)

hypertonic solution

17.

The normal method of reproduction of bacteria is:

a)

fragmentation

b)

binary fission

c)

transduction

d)

budding

18.

Mesophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

19.

Thermophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

20.

Hyperthermophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

21.

Psychrophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

22.

Psychrotroph:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

23.

Acidophile:

a)

microbes that grow best at pH>7

b)

microbes that grow best at pH<7

c)

microbes that grow best at pH=7

24.

Osmophile:

a)

sugar-loving microbe

b)

salt-loving microbe

c)

methane-loving microbe

25.

Halophile:

a)

sugar-loving microbe

b)

salt-loving microbe

c)

methane-loving microbe