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MYCOBACTERIUM 1

Total questions: 10

Worksheet time: 6mins

Name
Class
Date
1.

These colonies are characteristics of what Mycobacterial species?

a)

M. tuberculosis

b)

M. leprae

c)

M. avium complex

d)

M. kansasii

2.

Culture media that is recommended as source of inoculum for niacin test.

(Do not include the words "media or medium" to save time.)

(a)  

3.

In the zephiran-trisodium phosphate technique of decontamination and digestion, which substance speeds up/shortens the process?

a)

dithiothreitol

b)

5% oxalic acid

c)

trisodium phosphate

d)

Benzalkonium chloride

4.

Which of the following tuberculin skin test methods involve a piece of cloth soaked in OT/PPD and placed over skin?

a)

Mantoux

b)

Von pirquet

c)

Vollmer

d)

Moro percutaneous

5.

These are fluorochrome stains that can be used to identify M. tuberculosis bacilli.

a)

Acridine orange, Acridine yellow

b)

Auramine-rhodamine

c)

Auramine O, Blancophor R

d)

Carbolfuchsin, Methylene Blue

6.

According to the RITM/DOH scale, how will you report the following finding:             8 AFB / 100 visual fields

(a)  

7.

Which of the following choices does NOT belong to the group?

a)

Petragnani

b)

Lowenstein-Jensen

c)

Mitchison's

d)

American Thoracic society

8.

Positive color reaction for Niacin test.

(a)  

9.

Which of the following non-tuberculous Mycobacterium are able to produce pigmented colonies both in light and in dark environments?

a)

M. xenopi & M. gordonae

b)

M. kansasii & M. marinum

c)

M. fortuitum & M. chelonei

d)

M. avium & M. intracellulare

10.

Identify the organism and culture media used

a)

M. leprae on Petragnani

b)

M. tuberculosis on Lowenstein-Jensen medium

c)

M. avium complex on Lowenstein-Jensen

d)

M. kansasii on Middlebrook 7H11