WorksheetsSAMPLE PROCESSING MICROBIOLOGY
Total questions: 24
Worksheet time: 14mins
Why is specimen collection and transportation important for microbiology lab?
Specimen quality and its condition on arrival affects end results
Ensure the safety of lab staff
Allow better storage of unused reagents
Minimize error during sample processing
We should consider these factors when transporting a specimen, EXCEPT
Transport ASAP
Using a leak proof container
Aware of special preservatives
Aware of biohazard label
Do paperwork alongside with transportation
These are the criteria of specimen rejection EXCEPT
Insufficient quantity
Using an inappropriate container
Contamination of specimen
Unknown time delay
Adequate information labelled
Which one is the appropriate container for FAECES?
Which one is the appropriate container for URINE?
Which one is the appropriate container for BLOOD?
Which one is the appropriate container for NASOPHARYNGEAL SAMPLE?
Why do we perform sterility test?
To ensure no presence of microorganisms on sterile products
To ensure alcohol-free sterile products
To allow substance diffusion from the sterile products
To allow multipurpose use of sterile products
Sterility test define as .. ?
Microbiology test to confirm the products either sterile or not
Serology test to confirm the products either sterile or not
Blood test to confirm the products either sterile or not
Microscopic test to confirm the products either sterile or not
We can destroy microorganism by using .... methods.
Sterilization
Disinfection
Specification
Multipurpose
Disinfection ... ?
Destroy all microbial form
Destroy most of microbial form
Destroy few microbial form
Does not destroy microbial form at all
Sterilization ... ?
Destroy all microbial form
Destroy most of microbial form
Destroy few microbial form
Does not destroy microbial form at all
Choose the CORRECT method of disinfection.
UV radiation
Vapor
Direct transfer
Membrane filtration
Boiling
These factors can affect disinfection and sterilization efficacy, EXCEPT
Volume of disinfectant used
Type and level of microbial contamination
Temperature and pH of the disinfection process
Prior cleaning of the objects
Media that is used in sterility test.
Fluid thioglycolate media
Soybean casein digest broth media
Trypticase soy agar
Tryptic soy broth
We use this media to detect fungal and molds during sterility test
Fluid thioglycolate media
Soybean casein digest broth media
Trypticase soy agar
Tryptic soy broth
We use this media to detect aerobic and anaerobic bacteria during sterility test
Fluid thioglycolate media
Soybean casein digest broth media
Trypticase soy agar
Tryptic soy broth
Choose CORRECT methods used in sterility test.
Direct transfer
Indirect transfer
Membrane filtration
Membrane fertilization
We use ... method when we are doing sterility test for antimicrobial agents.
Direct transfer
Indirect transfer
Membrane filtration
Membrane fertilization
How does membrane filtration method works for sterility test?
Membrane filter retain most of bacteria
Membrane filter remove all bacteria
Membrane filter does not remove any bacteria
Incubate specimen with membrane filter
Which one is CORRECT regarding incubation period for bacteria and yeast?
Bacteria : 20-25'C, 14 days
Bacteria : 30-35'C, 14 days
Yeast : 20-25'C, 14 days
Yeast : 30-35'C, 14 days
When we perform sterility test for OILY specimen, we must .... to allow easier dispersion of specimen to media.
Emulsify
Oxidize
Dilute
Suspension
How can we perform sterility test for CATHETERS?
Cut into pieces
Fill lumen with medium
Wrap in plastic
Pour medium on top of catheter
How can we perform sterility test for SURGERICAL DRESSING?
Cut one sample from center of dressing
Cut two samples from different region of dressing
Cut three samples from different region of dressing
Put the entire dressing
