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WorksheetsRecombinant DNA Technology
Total questions: 16
Worksheet time: 8mins
Recombinant DNA technology is used:
For production of herbicide-resistant plant.
To make a particular protein.
To clone a specific piece of DNA
All of the above.
Restriction enzymes found in bacterial cells are naturally used:
During DNA replication.
To degrade the bacterial cell's DNA.
To degrade viral DNA that enters the cell.
To attach pieces of DNA together.
Which of the following is correct about host cell?
Plasmid is an example of host cell.
Unable to receive DNA molecule through transformation.
An organism that prevents cloning vectors from multiplying.
An organism that is used to receive recombinant DNA for cloning purposes.
What is the purpose of amplification?
To screen for recombinant bacteria.
To join the rDNA with the host cell.
To produce multiple copies of the desired gene.
To prevent the bacteria from reproducing.
Which of these pairs is incorrectly matched?
DNA ligase – mapping human chromosomes
Target DNA – gene of interest
Taq polymerase – PCR
Plasmid as a vector – isolated from bacteria
Which of these might be a biotechnology product?
Vaccine.
Modified enzyme.
Protein hormone.
All of the above.
Plants are more readily manipulated by genetic engineering than are animals because:
Plant genes do not contain introns.
A somatic plant cell can often give rise to a complete plant.
Genes can be inserted into plant cells by microinjection.
Plant cells have larger nuclei.
The following are steps involved in gene cloning procedures. Rearrange them into the correct sequence:
I. Transformation and amplification.
II. Screen for bacteria that contain recombinant
DNA.
III. Insert DNA fragment into the cut bacterial
plasmid.
IV. Cleave the plasmid and human DNA with the same restriction enzyme.
V. Isolate the bacterial plasmid and human DNA.
A. I, II, III, IV, V
B. V, IV, III, I, II
C. V, IV, III, II, I
D. IV, V, III, I, II
Which of the following is true regarding screening by using antibiotics and X-gal?
I. Antibiotics will prevent the growth of bacteria
without plasmid.
II. Bacterial cells with recombinant plasmids are
resistant to antibiotics and are unable to hydrolyse
X-gal.
III. Bacterial cells which contain non-recombinant
plasmids are resistant to antibiotics and are unable
to hydrolyse X-gal.
I only
I and II only
II and III only
I, II and III
Which best describes plasmids?
Plasmids are enzymes used to cut DNA at specific locations
Plasmids are transgenic bacteria
Plasmids are circular pieces of DNA that can be used to carry foreign DNA into cells such as bacteria
Plasmids are clones of bacteria or DNA segments.
Which of the following statements is true about developing cDNA?
mature mRNA directs the formation of the DNA
mature mRNA does not contain introns
DNA taken from the nucleus is used to produce the cDNA
Both a and b are true
The unpaired nucleotides produced by the action of restriction enzymes are referred to have
sticky ends
single strands
restriction fragments
ligases
Restriction endonucleases cut DNA at a specific site called
ligation site
ori
recognition sequence
replication site
Which one of the following statements about Restriction Endonuclease is true
all restriction endonucleases cut DNA at specific sites
all restriction endonucleases cut DNA at random sites
all restriction endonucleases join DNA segments at specific sites
all restriction endonucleases join DNA at random sites
In genetic engineering, DNA ligase is used as:
a probe
a sealing enzyme
a restriction enzyme
a mutagen
In screening process, colonies that were white in the presence of X-gal contained:
Plasmid DNA only
Foreign DNA only
Both foreign DNA and plasmid
Neither plasmid nor foreign DNA
