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Microscopy

Total questions: 20

Worksheet time: 26mins

Name
Class
Date
1.

Find total magnification for:

Eyepiece = 10X

Objective lens = 4X

a)

4x

b)

6x

c)

14x

d)

40x

2.
What is number 13?
a)
revolving nosepiece
b)
eyepiece
c)
objective lens
d)
base
3.
What is number 19?
a)
diaphragm
b)
coarse adjustment
c)
fine adjustment
d)
light source
4.
If your teacher asks you to move a microscope to table, how do you carry it?
a)
by the body tube and the eyepiece
b)
with one hand
c)
one hand on the arm and the other under the base
d)
with one hand on the base and the other on the eyepiece.
5.
Holds the objectives, used to switch between magnifications (low, high, and scanning)
a)
revolving nosepiece
b)
stage
c)
arm
d)
base
6.
Find the total magnification:
Eyepiece = 15X 
Objective lens = 100X
 
a)
15x
b)
100x
c)
115x
d)
1500x
7.

Which of the following does NOT describe a microscope? It _________.

a)

decreases the size of small objects

b)

increases the size of small objects

c)

makes small objects appear larger

d)

makes faraway objects look closer

8.

Which of the following is an illuminating part of the compound microscope?

a)

Arm

b)

Base

c)

Mirror

d)

Nosepiece

9.

A light microscope is also referred to as a?

a)

Electron microscope

b)

Compound microscope

c)

Scanning problem microscope

d)

X-ray

10.

Why is it important that an object is very small or thin in light microscopy?

a)

Light needs to transmit through the sample.

b)

The object needs to go into the objective lens.

c)

It needs to be heated quickly.

d)

None of the choices; the object should be large.

11.

Base your answer to the following question on the diagram and on your knowledge of biology.

a)

testing a specimen for amino acids

b)

determining the pH of a specimen

c)

measuring the photosynthetic rate in a specimen

d)

preparing a wet mount of a specimen

12.

When light is converted into heat, we can say that the light is ____________

a)

Refracted

b)

Reflected

c)

Absorbed

d)

Transmitted

13.

A type of microscopy that uses fluorescent dye that emits fluorescence when illuminated with ultraviolet

radiation.

a)

Electron microscopy

b)

Fluorescence microscopy

c)

Phase contract microscopy

d)

Dark-field microscopy

14.

Microscopy that is ideally used to illuminate unstained samples causing them to appear brightly lit against a dark background.

a)

Electron microscopy

b)

Fluorescence microscopy

c)

Phase contract microscopy

d)

Dark-field microscopy

15.

Which statement is true for Bright-field microscopy?

a)

Both diffracted and undiffracted lights are collected by the objective lens and thus, producing a very bright background.

b)

The specimens are not stained with dyes.

c)

Objective lens collects only the diffracted light that produces a bright image and background

d)

Bright-field microscope needs more light compared to other types of microscope.

16.

What type of microscope produces such image?

a)

Bright-field Microscope

b)

Dark-field Microscope

c)

Phase Contrast Microscope

d)

Electron Microscope

17.

Which image is produced by phase-contrast microscope?

a)

A

b)

B

c)

C

d)

None

18.

A microscope that uses a beam of accelerated electrons as a source of illumination

a)

Electron microscopy

b)

Fluorescence microscopy

c)

Phase contract microscopy

d)

Dark-field microscopy

19.

Three essential systems of Transmission Electron Microscope.

4 lines
20.

Which is the correct order for SEM sample preparation?

a)

Dehydration, Fixation, Drying, Metal coating

b)

Metal coating, Dehydration, Fixation, Drying

c)

Fixation, Dehydration, Drying, Metal coating

d)

Drying, Dehydration, Fixation, Metal coating