WorksheetsClassical methods of DNA sequencing
Total questions: 10
Worksheet time: 5mins
DNA sequencing refers to the
the technique used to determine the sugar sequence in a DNA molecule
the technique used to determine the phosphate sequence in a DNA molecule
the technique used to determine the base sequence in a DNA molecule
All of these
Which of the following is chemical nucleotide sequencing method
Sanger method
Maxam Gilbert method
Edmans method
Automated sequencing method
The enzyme used in Maxam-Gilbert method for p32 labelling of the DNA at 3' end is
polynucleotide kinase
alkaline phosphatase
exonuclease
terminal nucleotidyl trabsferase
How many different types of chemical treatments are required in Maxam-Gilbert Method
1
2
3
4
Guanine specific cleavage in Maxam-Gilbert method is done by using
formic acid
hydrazine
dimethyl sulphate
Piperidine
The principle of Sanger's method relies on
use of chemicals for base specific cleavage
use of dNTPs for chain termination
use of ddNTPs for chain termination
Use of p32 for chain termination
The samples in Sanger's method after reaction are separated using
AGE
PAGE
PFGE
2-D gel electrophoresis
Which of the following sequencing method uses PCR for generating sequence templates
Sanger's method
Sanger's method and LMPCR
LMPCR
LMPCR and automated DNA sequence
Automated DNA sequencing is an improvement of Sanger's method where
ddNTPs are used for chain termination
PCR is used for making sequencing
Fluorescently labelled dNTPs are used for chain termination
Fluorescently labelled ddNTPs are used for chain termination
Which of the following is not a DNA sequencing method
LMPCR
Edmans method
Sanger's method
Maxam-Gilbert method
