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Worksheets

Clinical Chemistry

Total questions: 50

Worksheet time: 50mins

Name
Class
Date
1.

Conversion factor for Thyroxine:

a)

0.357

b)

12.9

c)

10

2.

A substance that can yield hydrogen ion. (H+) or hydronium ion when dissolved in water.

a)

Base

b)

Acid

c)

Superbase

3.

A substance that can yield hydroxyl ions (OH-)

a)

Base

b)

Acid

c)

Superbase

4.

Expression of one amount relative to another amount:

a)

Ratio

b)

Dilution

c)

Concentration

5.

The amount of one substance relative to the amounts of other substances in the solution:

a)

Ratio

b)

Dilution

c)

Concentration

6.

Relative Concentration of the components of the mixture:

a)

Ratio

b)

Dilution

c)

Concentration

7.

Beer's Law states that the concentration of the unknown substance is:

a)

Directly proportional to the absorbed light (absorbance or optical density)

b)

Inversely proportional to the amount of transmitted light (% transmittance)

c)

Both of these

8.

It is considered as the most common type of photodetector. It has EXCELLENT SENSITIVITY and very rapid response. It detects very low levels of light.

a)

Barrier layer cell

b)

Photomultiplier (MP) tube

c)

Photoiode

9.

Assay for urea nitrogen that is more specific but more expensive:

a)

Colorimetric: Diacetyl

b)

Enzymatic: Ammonial formation

c)

Metabolism

10.

Tests of ammonia, urea and uric acid assess which function of the kidney?

a)

Synthesis

b)

Excretion

c)

Metabolism

11.

Assay for creatinine that is simple but nonspecific:

a)

Colorimetric: kinetic

b)

Colorimetric: Endpoint

c)

Enzymatic

12.

Assay for creatinine that is rapid and with increased specificity:

a)

Colorimetric: kinetic

b)

Colorimetric: endpoint

c)

Enzymatic

13.

Test for creatinine that measures ammonia colorimetrically or with ion-selective electrode:

a)

Enzymatic

b)

Colorimetric: endpoint

c)

Colorimetric: kinetic

14.

Assay for urea nitrogen that is more specific but more expensive:

a)

Colorimetric: Diacetyl

b)

Metabolism

c)

Enzymatic: ammonia formatuion

15.

Assess the Synthetic function of the liver:

a)

Albumin

b)

Cholinesterase

c)

Coagulation proteins

d)

All of these

16.

Tests for bilirubin and ammonia assess which function of the liver:

a)

Synthesis

b)

Metabolism

c)

Excretion

d)

Detoxification

17.

Problems with Turbidity and several drug interference can be encountered with this assay for uric acid:

a)

Colorimetric

b)

Enzymatic: UV

c)

Enzymatic: H2O2

18.

Simplest and the most specific method for uric acid:

a)

Chemical: Redox reaction

b)

Enzymatic: Uricase method

c)

Isotope dilution mass spectrometry

19.

Condensation method for Glucose:

a)

Folin Wu: Phosphomolybdic acid

b)

Nelson Somogyi: arsenomolybdic acid

c)

Neocuproine

d)

Dubowski: Orthotoluidine

20.

C-peptide is formed during the conversion of pro-insulin to insulin. The amount of circulating C-peptide provides reliable indicators for pancreatic and insulin secretions ( β\beta - cell function). It is DECREASED in:

a)

Type 2 DM

b)

Type 1 DM

c)

Insulinoma

d)

Ingestion of hypoglycemic drugs

21.

Gestational diabetes apparently arises when the extra metabolic demands of the fetus put stress on the ability of the mother to produce adequate amount of insulin for proper glucose utilization. A large percentage of patients develop diabetes within:

a)

2 to 3 years

b)

5 to 10 years

c)

10 to 15 years

d)

15 to 20 years

22.

Laboratory test(s) for diabetic management:

1. Glycosylated hemoglobin (HbA1c)

2. Ketones

3. Microalbumin

a)

1 and 3

b)

2 and 3

c)

1, 2 and 3

d)

only 1

23.

If testing is done in none fasting samples, only total cholesterol (TC) and HDL-c can being measured, FASTING becomes a requirement.

a)

4 to 8 hours fasting

b)

8 to 12 hours fasting

c)

12 to 14 hours fasting

d)

16 to 18 hours fasting

24.

Glycosylated hemoglobin provides information about glucose metabolism over a six months period. The major approach(es) in its measurement is(are):

a)

Column chromatography

b)

Electrophoresis

c)

Both of these

25.

Colorimetric method for triglycerides:

a)

van Handel and Zilversmith

b)

Hantzch condensation

c)

Eric Erikson

d)

Ivan Pavlov and Bandura

26.

Fluorometric method for triglycerides:

a)

van Handel and Zilversmith

b)

Ivan Pavlov and Bandura

c)

Hantzch condensation

d)

Hilda Taba

27.

The simplest approach for cholesterol determination is the one-step direct method, known as the:

a)

Liebermann-Burchardt (L-B) procedure

b)

Abell-Kendall

c)

Kendal-Marsiano

28.

One-step method for cholesterol: (C)

a)

Pearson, stem and Mac Gavack

b)

Bloors

c)

Abell-Kendall

d)

Schoenheimer Sperry Parekh and Jung

29.

Two-step method for cholesterol: (C + E)

a)

Pearson, stem and Mac Gavack

b)

Abell-Kendall

c)

Bloors

d)

Schoenheimer Sperry, Parekh and Jung

30.

Three-step method for cholesterol: (CE+S)

a)

Pearson, stem and Mac Gavack

b)

Schoenheimer Sperry, Parekh and Jung

c)

Bloors

d)

Abell-Kendall

31.

Four-step method cholesterol: (CES+P)

a)

Bloors

b)

Abell-Kendall

c)

Schoenheimer Sperry, APrekh and Jung

d)

Pearson, stem and Mac Gavack

32.

Major structural protein in VLDL and LDL:

a)

Apo B-48

b)

Apo A-1

c)

Apo B-100

d)

Apo A-100

33.

Floating β\beta  lipoprotein:

a)

Lp (a)

b)

β\beta  -VLDL

34.

Sinking pre- β\beta   lipoprotein:

a)

Lp (a)

b)

β\beta  -VLDL

35.

LDL cholesterol can be calculated from measurements of all of the following except:

a)

Total cholesterol

b)

Triglycerides

c)

VLDL cholesterol

d)

HDL cholesterol

36.

Gilbert's syndrome:

a)

Bilirubin transport deficit

b)

Conjugation deficit

37.

Crigler-Najjar syndrome:

a)

Bilirubin transport deficit

b)

Conjugation deficit

38.

It is increased in hemolytic anemias:

a)

B2

b)

B3

c)

B1

d)

All of these

39.

it is increased in bile duct obstruction:

a)

B1

b)

B2

c)

B3

d)

All of these

40.

Increased in hepatitis:

a)

B1

b)

B2

c)

both fractions

41.

True for CHOLELITHIASIS:

a)

in adults, cholelithiasis is the most common cause of hyperbilirubinemia

b)

Presence of bile stones that are composed of bilirubin or cholesterol

c)

Results in elevation of total bilirubin with >90% being DIRECT BILIRUBIN

d)

all of these

42.

The tertiary structure of protein:

a)

linear sequence of amino acid

b)

winding of the polypeptide chain

c)

three-dimensional configuration, folding pattern of protein

d)

association of 2 or more polypeptide chains to form a functional protein molecule

43.

Indicator of MALNUTRITION, binds thyroid hormone and retinol-binding protein:

a)

Prealbumin

b)

Orosomucoid

c)

Ceruloplasmin

d)

Haptoglobin

44.

In the dye-binding method for serum albumin, which dye gives much greater absorbance at 630 nm than 500 nm. Absorbance at 630 nm is less likely to be affected by absorbance due to bilirubin or hemoglobin in the sample.

a)

BCG (bromcresol green)

b)

HABA (hyroxyazobenzene- benzoic acid)

45.

A condition associated with increased serum albumin (hyperalbuminemia):

a)

Chronic liver disease

b)

Dehydration

c)

Nephrotic syndrome

d)

Widespread malignancy

46.

ARTIFACTUAL increase in albumin concentration:

a)

Prolonged tourniquet time

b)

Dehydration

c)

Nephrotic syndrome

47.

It evaluates the degree of intravascular hemolysis, hemolytic disease of the newborn and hemolytic transfusion reactions:

a)

Ceruloplasmin

b)

Haptoglobin

c)

Prealbumin

d)

Transferrin

48.

Beta-gamma bridging effect:

a)

multiple myeloma

b)

Hepatic cirrhosis

c)

Nephrotic syndrome

d)

Juvenile cirrhosis (AAT deficiency)

e)

inflammation

49.

Standard reference method for protein determination, not commonly performed:

a)

Kjeldahl method

b)

Biuret method

c)

Folin-Ciocalteau (Lowry) method

d)

Ultraviolet absorption method

50.

Most widely used method for protein determination, recommended by the international. Federation of Clinical Chemist (IFCC) expert panel:

a)

Kjeldahl method

b)

Biuret method

c)

Folin-ciocalteau (lowry) method

d)

Ultraviolet abosorption method