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WorksheetsClinical Chemistry
Total questions: 50
Worksheet time: 50mins
Conversion factor for Thyroxine:
0.357
12.9
10
A substance that can yield hydrogen ion. (H+) or hydronium ion when dissolved in water.
Base
Acid
Superbase
A substance that can yield hydroxyl ions (OH-)
Base
Acid
Superbase
Expression of one amount relative to another amount:
Ratio
Dilution
Concentration
The amount of one substance relative to the amounts of other substances in the solution:
Ratio
Dilution
Concentration
Relative Concentration of the components of the mixture:
Ratio
Dilution
Concentration
Beer's Law states that the concentration of the unknown substance is:
Directly proportional to the absorbed light (absorbance or optical density)
Inversely proportional to the amount of transmitted light (% transmittance)
Both of these
It is considered as the most common type of photodetector. It has EXCELLENT SENSITIVITY and very rapid response. It detects very low levels of light.
Barrier layer cell
Photomultiplier (MP) tube
Photoiode
Assay for urea nitrogen that is more specific but more expensive:
Colorimetric: Diacetyl
Enzymatic: Ammonial formation
Metabolism
Tests of ammonia, urea and uric acid assess which function of the kidney?
Synthesis
Excretion
Metabolism
Assay for creatinine that is simple but nonspecific:
Colorimetric: kinetic
Colorimetric: Endpoint
Enzymatic
Assay for creatinine that is rapid and with increased specificity:
Colorimetric: kinetic
Colorimetric: endpoint
Enzymatic
Test for creatinine that measures ammonia colorimetrically or with ion-selective electrode:
Enzymatic
Colorimetric: endpoint
Colorimetric: kinetic
Assay for urea nitrogen that is more specific but more expensive:
Colorimetric: Diacetyl
Metabolism
Enzymatic: ammonia formatuion
Assess the Synthetic function of the liver:
Albumin
Cholinesterase
Coagulation proteins
All of these
Tests for bilirubin and ammonia assess which function of the liver:
Synthesis
Metabolism
Excretion
Detoxification
Problems with Turbidity and several drug interference can be encountered with this assay for uric acid:
Colorimetric
Enzymatic: UV
Enzymatic: H2O2
Simplest and the most specific method for uric acid:
Chemical: Redox reaction
Enzymatic: Uricase method
Isotope dilution mass spectrometry
Condensation method for Glucose:
Folin Wu: Phosphomolybdic acid
Nelson Somogyi: arsenomolybdic acid
Neocuproine
Dubowski: Orthotoluidine
C-peptide is formed during the conversion of pro-insulin to insulin. The amount of circulating C-peptide provides reliable indicators for pancreatic and insulin secretions ( β - cell function). It is DECREASED in:
Type 2 DM
Type 1 DM
Insulinoma
Ingestion of hypoglycemic drugs
Gestational diabetes apparently arises when the extra metabolic demands of the fetus put stress on the ability of the mother to produce adequate amount of insulin for proper glucose utilization. A large percentage of patients develop diabetes within:
2 to 3 years
5 to 10 years
10 to 15 years
15 to 20 years
Laboratory test(s) for diabetic management:
1. Glycosylated hemoglobin (HbA1c)
2. Ketones
3. Microalbumin
1 and 3
2 and 3
1, 2 and 3
only 1
If testing is done in none fasting samples, only total cholesterol (TC) and HDL-c can being measured, FASTING becomes a requirement.
4 to 8 hours fasting
8 to 12 hours fasting
12 to 14 hours fasting
16 to 18 hours fasting
Glycosylated hemoglobin provides information about glucose metabolism over a six months period. The major approach(es) in its measurement is(are):
Column chromatography
Electrophoresis
Both of these
Colorimetric method for triglycerides:
van Handel and Zilversmith
Hantzch condensation
Eric Erikson
Ivan Pavlov and Bandura
Fluorometric method for triglycerides:
van Handel and Zilversmith
Ivan Pavlov and Bandura
Hantzch condensation
Hilda Taba
The simplest approach for cholesterol determination is the one-step direct method, known as the:
Liebermann-Burchardt (L-B) procedure
Abell-Kendall
Kendal-Marsiano
One-step method for cholesterol: (C)
Pearson, stem and Mac Gavack
Bloors
Abell-Kendall
Schoenheimer Sperry Parekh and Jung
Two-step method for cholesterol: (C + E)
Pearson, stem and Mac Gavack
Abell-Kendall
Bloors
Schoenheimer Sperry, Parekh and Jung
Three-step method for cholesterol: (CE+S)
Pearson, stem and Mac Gavack
Schoenheimer Sperry, Parekh and Jung
Bloors
Abell-Kendall
Four-step method cholesterol: (CES+P)
Bloors
Abell-Kendall
Schoenheimer Sperry, APrekh and Jung
Pearson, stem and Mac Gavack
Major structural protein in VLDL and LDL:
Apo B-48
Apo A-1
Apo B-100
Apo A-100
Floating β lipoprotein:
Lp (a)
β -VLDL
Sinking pre- β lipoprotein:
Lp (a)
β -VLDL
LDL cholesterol can be calculated from measurements of all of the following except:
Total cholesterol
Triglycerides
VLDL cholesterol
HDL cholesterol
Gilbert's syndrome:
Bilirubin transport deficit
Conjugation deficit
Crigler-Najjar syndrome:
Bilirubin transport deficit
Conjugation deficit
It is increased in hemolytic anemias:
B2
B3
B1
All of these
it is increased in bile duct obstruction:
B1
B2
B3
All of these
Increased in hepatitis:
B1
B2
both fractions
True for CHOLELITHIASIS:
in adults, cholelithiasis is the most common cause of hyperbilirubinemia
Presence of bile stones that are composed of bilirubin or cholesterol
Results in elevation of total bilirubin with >90% being DIRECT BILIRUBIN
all of these
The tertiary structure of protein:
linear sequence of amino acid
winding of the polypeptide chain
three-dimensional configuration, folding pattern of protein
association of 2 or more polypeptide chains to form a functional protein molecule
Indicator of MALNUTRITION, binds thyroid hormone and retinol-binding protein:
Prealbumin
Orosomucoid
Ceruloplasmin
Haptoglobin
In the dye-binding method for serum albumin, which dye gives much greater absorbance at 630 nm than 500 nm. Absorbance at 630 nm is less likely to be affected by absorbance due to bilirubin or hemoglobin in the sample.
BCG (bromcresol green)
HABA (hyroxyazobenzene- benzoic acid)
A condition associated with increased serum albumin (hyperalbuminemia):
Chronic liver disease
Dehydration
Nephrotic syndrome
Widespread malignancy
ARTIFACTUAL increase in albumin concentration:
Prolonged tourniquet time
Dehydration
Nephrotic syndrome
It evaluates the degree of intravascular hemolysis, hemolytic disease of the newborn and hemolytic transfusion reactions:
Ceruloplasmin
Haptoglobin
Prealbumin
Transferrin
Beta-gamma bridging effect:
multiple myeloma
Hepatic cirrhosis
Nephrotic syndrome
Juvenile cirrhosis (AAT deficiency)
inflammation
Standard reference method for protein determination, not commonly performed:
Kjeldahl method
Biuret method
Folin-Ciocalteau (Lowry) method
Ultraviolet absorption method
Most widely used method for protein determination, recommended by the international. Federation of Clinical Chemist (IFCC) expert panel:
Kjeldahl method
Biuret method
Folin-ciocalteau (lowry) method
Ultraviolet abosorption method
