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Endonucleases, ligases and polymerases

Total questions: 10

Worksheet time: 10mins

Name
Class
Date
1.

Which type of enzyme act as genetic scissors?

a)

Ligases

b)

Restriction Enzymes

c)

Endonucleases

d)

Polymerases

2.

Which enzyme acts as genetic glue?

a)

Ligases

b)

Restriction enzymes

c)

Endonucleases

d)

Polymerases

3.

Which enzymes act to build polymers from their monomers?

a)

Ligases

b)

Restriction enzymes

c)

Endonucleases

d)

Polymerases

4.

Which suffix is most commonly found on the name of an enzyme?

a)

-ide

b)

-ase

c)

-ose

d)

-itis

5.

An endonuclease has cut a fragment of DNA directly across. Which end is the result?

a)

Sticky-end

b)

Blunt-end

6.

An endonuclease has cut a fragment of DNA in a staggered manner. Which end is the result?

a)

Sticky-end

b)

Blunt-end

7.

Which type of cut DNA is easier to work with?

a)

Sticky-end

b)

Blunt-end

8.

Explain why blunt-end DNA fragments are harder to work with in biotechnology.

4 lines
9.

Which type of enzyme can build DNA molecules?

a)

RNA Polymerase

b)

DNA Ligase

c)

DNA Endonuclease

d)

DNA Polymerase

10.

Explain how endonucleases and ligases work well together.

4 lines