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WorksheetsIntroduction to Histology
Total questions: 73
Worksheet time: 39mins
In preparing tissue for routine light microscopic study, which procedure immediately precedes clearing the specimen with an organic solvent?
Dehydration
Fixation
Staining
Clearing
He discovered the cellula in a piece of cork during 1665
Robert Hooke
Robert Brown
Rudolf Virchow
Rudolph Albert Von Kolliker
He verified the organelle and introduction of the term nucleus.
Robert Hooke
Robert Brown
Rudolf Virchow
Rudolph Albert Von Kolliker
He established the fundamental law of biology
Robert Hooke
Robert Brown
Rudolf Virchow
Rudolph Albert Von Kolliker
Rudolf Virchow stated "Omnis cellular e cellular" which means "All cells arise in pre-existing cells"
TRUE
FALSE
He published the 1st textbook in Histology
Robert Hooke
Robert Brown
Rudolf Virchow
Rudolph Albert Von Kolliker
Father of Microscopic Anatomy and Embryology
Anton van Leeuwenhoek
Jan Evangelista Purkinje
Marcello Malpighi
Theodore Schwann
He established the cell theory
Anton van Leeuwenhoek
Jan Evangelista Purkinje
Marcello Malpighi
Theodore Schwann
He described the nuclear membrane and introduce the term " protoplasm"
Anton van Leeuwenhoek
Jan Evangelista Purkinje
Marcello Malpighi
Theodore Schwann
He described the nucleus and is known for his invention of the microscope (single-lens microscope)
Anton van Leeuwenhoek
Jan Evangelista Purkinje
Marcello Malpighi
Theodore Schwann
He observed the cell
Anton van Leeuwenhoek
Jan Evangelista Purkinje
Marcello Malpighi
Theodore Schwann
Founder of Animal Histology
Rudolph Albert Von Kolliker
Marie Francois Xavier Bichat
Adolf Mayer
Rudolf Virchow
He created the term "Histology"
Rudolph Albert Von Kolliker
Marie Francois Xavier Bichat
Adolf Mayer
Rudolf Virchow
He discovered "Tissue"
Rudolph Albert Von Kolliker
Marie Francois Xavier Bichat
Adolf Mayer
Rudolf Virchow
Histology is derived from the Latin word "Histos= tissue" and "Logos= study"
False
True
It deals with the study of microscopic structures of the cells and tissues in plants and animals
Histology
Microbiological Anatomy
Histopathology
Histology is essentially known as (a) .
Histology is the study of the extracellular matrix of tissues. It also studies the microscopic structures of biological material and the ways in which individual components are functionally related
True
False
HISTOLOGY is the diseased or injured tissues while HISTOPATHOLOGY is the normal tissues.
True
False
HISTOLOGY is the normal tissues while HISTOPATHOLOGY is the diseased or injured tissues.
True
False
The methods of preparation of tissues and cells for microscopic examination are called microtechniques. Microtechniques are the different methods used for the preparation of histological sections.
All statements are TRUE
All statements are FALSE
Only 1st statement is TRUE
Only 2nd statement is TRUE
It is a slice of tissue
Piece
Section
Specimen
Species
It is the spreading of blood film, connective tissue, and bone marrow over glass slide.
Fresh Tissue Examination
Fresh Specimen Examination
Fresh Sample Examination
Fresh Section Examination
Cutting the organ into small pieces or very thin slices (4 - 8µm)
Preparation of species from organs
Preparation of selections from organs
Preparation of sections from organs
Preparation of specimen from organs
What are the 2 methods for preparing a section?
Paraffin and Cooling Method
Boiling and Heating Method
Paraffin and Freezing Method
Iced and Water Bath Method
It is prepared by cutting a specimen imbedded in a paraffin block by a rotary microtome
Freezing Method
Paraffin Method
Fresh Tissue Examination
Trimming Method
What is the trimming measures of paraffin section?
2-6µm
4-5µm
6-8µm
5-6µm
Section is prepared by cutting fresh specimen by a freezing microtome
Refrigerating Method
Cooling Method
Freezing Method
Slush Ice Method
Which of the following is not a process of preparing a sections from organs?
Mouthing
Labeling
Fixation
Impregnation
It is the process of assigning the tissue with its corresponding identification or accession number.
Lettering
Numbering
Ordering
Analyzing
It is the process of preserving tissue or materials for histopathologic study and considered as the most critical part of tissue processing using the paraffin method?
Numbering
Fixation
Decalcification
Dehydration
It is the process of removing intercellular and extracellular water from the tissue
Numbering
Fixation
Decalcification
Dehydration
It is the removal of calcium ion from the tissue and the only tissue processing that can be removed or omitted?
Numbering
Fixation
Decalcification
Dehydration
It is the process whereby alcohol is removed from the tissue and replaced by a substance that can dissolve the wax in which tissue is impregnated.
Dealcoholization
Impregnation
Section-Cutting
Embedding
It is the process by which the impregnated tissue is placed into a precisely arranged position in a mold containing medium and allowed to solidify?
Dealcoholization
Impregnation
Section-Cutting
Embedding
It is the removal of a clearing agent.
Dealcoholization
Impregnation
Section-Cutting
Embedding
It is the process whereby tissue are cut into uniformly thin slices or section and usually done by the aid of a microtome
Dealcoholization
Impregnation
Section-Cutting
Embedding
It is the process in which the tissue is artificially colored?
Mounting
Labeling
Numbering
Staining
It is the process in which cover slip is applied over the stained tissue section and usually done for the purpose of preservation of tissue slides?
Mounting
Labeling
Numbering
Staining
It is the process in which the assigned accession or identification code is permanently attached to the slide and ready for microscopic evaluation.
Mounting
Labeling
Numbering
Staining
Microscope came from the Greek word "micron" which is "small" and "skopein" which means "to look at"?
True
False
An optical instrument that uses a lens or a combination of lenses to produce magnified images of small objects, especially of objects too small to be seen by the unaided eye
(a)
Optical and Mechanical Parts are the general parts of the compound microscope.
False
True
Identify the part 9 of the microscope
(a)
Identify the part 12 of the microscope
(a)
Identify the part 2 of the microscope
(a)
Part of the microscope where one peeps or looks to see the specimen under study and usually contains lens that can magnify 10x?
Body Tube
Draw Tube
Ocular
Objectives
holds the dust shield and the revolving nosepiece
Body Tube
Draw Tube
Ocular
Objectives
holds the eyepiece or ocular
Body Tube
Draw Tube
Ocular
Objectives
Holds the objective lens.
Revolving Nosepiece
Scanning Objectives
Dust Shield
Low Power Objective
Protects the objective lens from dust and dirt
Revolving Nosepiece
Scanning Objectives
Dust Shield
Low Power Objective
The longest objective; usually marked 100x and used to view structures using oil as a medium.
Oil Immersion Objective
Scanning Objectives
High Power Objective
Low Power Objective
The shortest objective and usually marked 4x
Oil Immersion Objective
Scanning Objectives
High Power Objective
Low Power Objective
Used to see the general outline of the specimen usually marked 10x
Oil Immersion Objective
Scanning Objectives
High Power Objective
Low Power Objective
Used to view structures in a larger perspective usually marked 40x
Oil Immersion Objective
Scanning Objectives
High Power Objective
Low Power Objective
Holds and secures the slide
Mechanical Adjustment Knob
Fine Adjustment Knob
Stage
Stage Clip
It is a part of the microscope where the glass slide is placed.
Mechanical Adjustment Knob
Fine Adjustment Knob
Stage
Stage Clip
It rapidly raises or lowers the stage or objective to initially bring the specimen into focus.
Mechanical Adjustment Knob
Fine Adjustment Knob
Coarse Adjustment Knob
Iris Diaphragm
It used for more precise focusing.
Mechanical Adjustment Knob
Fine Adjustment Knob
Coarse Adjustment Knob
Iris Diaphragm
It moves the stage or slide from left to right and from top to bottom.
Mechanical Adjustment Knob
Fine Adjustment Knob
Coarse Adjustment Knob
Iris Diaphragm
Regulates the amount of light entering the stage.
Mechanical Adjustment Knob
Fine Adjustment Knob
Condenser
Iris Diaphragm
Concentrated the amount of light entering the stage.
Mechanical Adjustment Knob
Fine Adjustment Knob
Condenser
Iris Diaphragm
Lens nearest to the specimen
Objective Lens
Ocular Lens
Binocular Lens
Monocular Microscope
Lens nearest to the viewer
Objective Lens
Ocular Lens
Binocular Lens
Monocular Microscope
The specimen is unstained but the background is dark in shade and used in the diagnosis of syphilis.
Light Microscope
Fluorescence Microscope
Electron Microscope
Darkfield Microscope
The source of light is ultraviolet light and commonly used in immunodiagnostic tests.
Light Microscope
Fluorescence Microscope
Electron Microscope
Darkfield Microscope
The source of light is visible light.
Light Microscope
Fluorescence Microscope
Electron Microscope
Darkfield Microscope
The structures are colored and the background is clear or lighter in shade.
Brightfield Microscope
Fluorescence Microscope
Electron Microscope
Darkfield Microscope
The source of light is an electron beam and it can be a scanning EM or transmission EM. It is mainly used in the study of viruses.
Brightfield Microscope
Fluorescence Microscope
Electron Microscope
Darkfield Microscope
Which of the following is NOT a components of the body
Cells
Instacellar
Body Fluids
Intercellular and Extracellular
With true nucleus surrounded by a nuclear envelope
Prokaryotic
Eukaryotic
Lack of nuclear envelope and the nuclear substance is mixed with the rest of cytoplasm.
Prokaryotic
Eukaryotic
Fundamental structural units of living organisms and function in executing all of the activities that the body requires for its survival
Cell Wall
Nucleus
Cells
Cell Division
