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HPLC

Total questions: 10

Worksheet time: 4mins

Name
Class
Date
1.

What is HPLC?

a)

A technique to separate components based on pH

b)

A technique to separate components based on polarity

c)

A technique to separate components based on liquidity

d)

A technique to separate components based on temperatue

2.

C18 and silica are stationary phased that used in ...

a)

Normal and Normal HPLC respectively

b)

Normal and Reverse HPLC respectively

c)

Reverse and Normal HPLC respectively

d)

Reverse and Reverse HPLC respectively

3.

What is the function of degasser?

a)

To add gas into the system of HPLC

b)

To prevent gas into the system of HPLC

c)

To change liquid into gas

d)

To change gas into liquid

4.

What is the common pump used in HPLC?

a)

Syringe pump

b)

Plunger pump

c)

Diaphragm pump

5.

Which substance is used in normal phased HPLC?

a)

stationary phase: high polar

mobile phase: low polar

b)

Stationary phase: high polar

Mobile phase: high polar

c)

Stationary phase: low polar

Mobile phase: low polar

d)

Stationary phase: low polar

Mobile phase: high polar

6.

Which eluent will be eluted first in reverse phase of HPLC?

a)

The least polar

b)

The highest polar

c)

The simplest compound

d)

The most complex compound

7.

Solid phase extraction is used to ...

a)

purify the samples from unwanted components

b)

purify the solvents from unwanted components

c)

purify the samples using low elution strength

d)

purify the samples using high elution strength

8.

Water / methanol is used as a mobile phase in ...

a)

Normal phase of HPLC

b)

Reverse phase of HPLC

9.

The ability of an analytical procedure to remain unaffected by small changes is called ...

a)

Linearity

b)

Robustness

c)

Quantitation Limit

d)

Range

10.

In which temperature the column oven has to be maintained?

a)

25-50 degrees

b)

30-50 degrees

c)

35-60 degrees

d)

40-60 degrees