II, VII,IX, X

Vitamin K dependent

Not consumed during clotting (present in plasma and serum, except Factor II)

Stable and well‑preserved in stored plasma

Precipitated with barium sulfate – absent in this “adsorbed plasma”

Synthesized in liver

XI, XII, HMWK, PK

Activated upon contact

Not consumed during clotting (present in plasma and serum)

Fairly stable

when activated by thrombin it is converted to fibrin which forms the clot

Fibrinogen group

Fibrinogen assay (measured in mg/dL: 200-400)

-it is converted to thrombin

by the prothrombinase complex (Xa, Va, PF3, and Ca ++)

splits fibrinogen , stimulates platelet aggregation, and activates co-factors and protein C

- Formerly known as Factor III

cofactor found in tissue which activates the extrinsic pathway and factor VII

found in most body tissues and particularly high levels found in the brain and lungs

formerly factor IV

functions mainly as a bridge between the PL surface of platelets and several coagulation factors

necessary for many steps in coagulation

Measured in Chemistry using serum or heparinized plasma (can use ISE)

–speeds the transformation of prothrombin to thrombin

sensitive to coumadin drugs

activated by tissue thromboplastin

activates factor X

VIII/vWf (factor VIII complex) is the total factor VIII molecule

VIII:C is the active portion of the molecule which functions in the clotting process

vWf:Ag is the subunit capable of antibody production that binds to endothelium and helps in normal platelet adhesion and function (primary hemostasis)

Becomes activated by combining with the PL(PF3) surface of the platelet in the presence of Ca++ to then activate factor X

•Test 5M urea solubility

Vitamin K dependent

Labile

Consumed in the clot

serine proteases

converted to kallikrein, a serine protease that connects the coagulation pathways to fibrinolysis with the help of factor XIIa in the presence of HMWK

Adsorbs onto the surface of the vessel