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WorksheetsMI 4.1.4 Quiz Review (Quizizz)
Total questions: 11
Worksheet time: 7mins
The purpose of SDS in protein electrophoresis is to
invert (flip) the hydrophobic regions to the outside of the protein.
catabolize (break down) protein into individual amino acids.
coat the protein with negative charges.
wash the protein.
denature (unfold) the protein.
The gel generally used in protein electrophoresis is
polyacrylamide.
2.0% agarose.
gelatin.
agar.
0.8% agarose.
Protein electrophoresis is different than nucleotide (DNA) electrophoresis in that in protein electrophoresis,
proteins are, by default, negatively charged.
proteins are usually a lot smaller.
proteins are usually linearized, not folded.
the units measured are in kDa.
current flows from positive to negative.
A sample containing proteins that have not been gone through column chromatography most likely will result in a gel that contains
two bands indicating GFP and beta-lactamase.
multiple bands indicating different types of proteins present.
a single band indicating pure GFP.
a single band indicating pure bacterial proteins.
multiple bands indicating fragmented or fractured GFP.
SDS stands for
sodium dimethyl sulfonate.
solvent dessicant solution.
soapy dishwater solution.
sodium dodecahedron salicylate.
sodium dodecyl sulfate.
The molecular weight of GFP is
26.9 kb.
26.9 kDa.
26.9 Da.
29.6 kDa.
not known.
Which substance is used to separate proteins by size?
sodium docecyl sulfate
calcium chloride
2-mercaptoethanol
polyacrylamide
wash buffer
2-mercaptoethanol is used primarily to
give proteins a negative charge.
convert the native form of proteins to the denatured form.
separate proteins by size.
break down proteins into amino acids.
purify proteins.
Protein electrophoresis is different from DNA electrophoresis in that ____________ in protein electrophoresis.
polyacrylamide is used
agarose is used
a vertical apparatus chamber is used
a horizontal apparatus chamber is used
the electric current flow is from positive to negative
To visualize proteins on a gel, it is best to
use SybrSafe.
refrigerate the gel overnight.
use a protein stain and agitate the gel for a few hours.
use a blue light box.
use a protein stain and view immediately.
In order to positively identify a protein of known weight on a gel, if is necessary to
measure the Rf values of the bands in a gel.
use a protein standard marker.
find the line of best fit on a graph.
use regular graph paper.
use a semilog graph paper.
