Free Printable biology

1.

.

1.who is regarded as the Father of tissue culture?

a)

a) Louis Pasteur

b)

b) Edward Jenner

c)

c) G.Haberlandt

d)

(d) Melchers et al.

2.

2.The term used to define the ability of a cell to generate entire individual is

a)

(a) Pleuripotent

b)

(b) Totipotent

c)

(c) Multipotent

d)

(d) Unipotent

3.

3.The phenomenon of reversion of mature cells to meristematic state leading to

callus formation is ............

a)

(a) Redifferentiation

b)

(b) Dedifferentiation

c)

(c) either (a) or (b)

d)

(d) none of these

4.

Micro propagation involves

a)

vegetative multiplication of plants by using micro organisms.

b)

vegetative multiplication of plants by using small explants

c)

vegetative multiplication of plants by using microspores

d)

Non -vegetative multiplication of plants by using microspores and megaspores

5.

5. In vitro is a .............

a)

a) Greek word

b)

b)Latin word

c)

c)English word

d)

d)None

6.

6.Haberland was the first person to culture plant cells using the mesophyll of ....

a)

a) Lantana camera

b)

b)Lathyrus odoratus

c)

c) Lamium purpureum

d)

d)Lawsonia inermis

7.

7. The tissue taken from selected plant transferred to a culture medium is known as .......

a)

a) Explant

b)

b)Callus

c)

c)Plantlet

d)

d)Thallus

8.

8. which of the following condition favours growing the Explant inoculated into culture tubes at ......

a)

22-25 degree celsius with illumination of light 2400 lux with 8-16 hrs of photo period

b)

22-28 degree celsius with illumination of light 2400 lux with 8-16 hrs of photo period

c)

22-28 degree celsius with illumination of light 2200 lux with 8-16 hrs of photo period

d)

22-28 degree celsius with illumination of light 2400 lux with 8-12 hrs of photo period

9.

9.Haberlandt was a .....

a)

a) Indian botanist

b)

b)Austrian botanist

c)

c)American botanist

d)

d)German botanist

10.

10. ........... is an undifferentited mass of tissue

a)

a)Thallus

b)

b)Explant

c)

c)callus

d)

d)None

11.

Assertion (A) : Sterilization helps to overcome microbes.

Reason (R) : Explants are autoclaved.

a)

(a) Both A and R are correct but R is not a correct explanation to A

b)

(b) R explains A

c)

(c) A is correct R is incorrect

d)

(d) Both A and R incorrect

12.

Identify the correct sequence regarding steps involved in PTC

a)

(a) Sterilization → Incubation → Inoculation → Embryogenesis → Hardening

b)

(b) Inoculation → Induction →Sterilization → Hardening → Embryogenesis

c)

(c) Induction → Incubation → Inoculation → Hardening → Sterilization

d)

(d) Sterilization → Inoculation → Incubation → Embryogenesis → Hardening

13.

Identify the incorrect statement:

(a) Explants are surface sterilized

(b) Nutrient media are autoclaved

(c) Culture rooms are UV radiated for 15 minutes

(d) Glasswares and accessories are autoclaved

a)

(a) a only

b)

(b) b and c

c)

(c) d only

d)

(d) none of the avove

14.

Which of the following condition favours callus induction?

a)

(a) Temperature of 25°C ± 5°C with 12 hours of photoperiod

b)

(b) Temperature of 25°C ± 2°C with 18 hours of photoperiod

c)

(c) Temperature of 25°C + 5°C with 14 hours of photoperiod

d)

(d) Temperature of 25°C ± 2°C with 16 hours of photoperiod

15.

Identify the plant tissue used for virus-free germplasm

a)

(a) Apical meristem

b)

(b) Intercalary meristem

c)

(c) Lateral meristem

d)

(d) Plate meristem

biology

Total questions: 15

Worksheet time: 8mins

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