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Bacteriology - Methods of Identification

Total questions: 40

Worksheet time: 23mins

Name
Class
Date
1.

All of the following are examples of special stains for endospore, except:

a)

Dorner’s

b)

Schaeffer & Fulton

c)

Glacial acetic acid

d)

Fischer-Conn

2.

Give an example of a Living Smear Preparation.

(a)  

3.

What is the purpose of a fixed state smear preparation?

(a)  

4.

This type of stain uses a single basic dye.

(a)  

5.

Special stain used for staining Flagella.

(a)  

6.

He discovered that crystal violet irreversibly stains certain bacteria but can be washed from others.

(a)  

7.

Decolorizer used in Burke’s Modification of Gram Staining.

(a)  

8.

What is the color of a Gram (-) organism after the addition of Gram’s Iodine?

(a)  

9.

What is the color of a Gram (+) organism after the addition of Safranin dye?

(a)  

10.

All cocci are gram (+) EXCEPT:

(a)  

11.

All bacilli are gram (-) EXCEPT:

(a)  

12.

This theory of Gram Staining states that in Gram (+) organisms, the isoelectric point is making them acidic; binds well with Crystal Violet (basic dye).

(a)  

13.

All of the following are ERRORS IN GRAM STAINING wherein Gram (+) organism becomes Gram (-), except:

a)

Acidic Gram’s Iodine

b)

Removal of MgRNA with precipitation from bile salts in media

c)

Overheating

d)

Inadequate decolorization

14.

This is a non–stain system to determine true Gram Stain reaction wherein there is a formation of string–like material indicates G (-) organisms

(a)  

15.

This is responsible for the acid fastness of mycobacteria.

(a)  

16.

All organisms are non-acid fast, EXCEPT:

(a)  

17.

The color of a Non-AFB organism after the addition of Acid Alcohol.

(a)  

18.

The color of an AFB organism after the addition of Methylene Blue.

(a)  

19.

This type of Acid Fast Staining uses heat as a mordant.

(a)  

20.

This type of Acid Fast Staining uses tergitol as a mordant.

(a)  

21.

This acid fast stain differentiates M. tb from M. lacticala and M. smegmatis.

(a)  

22.

It is defined as the population of microorganisms or cells that is introduced in the fermentation medium or any other suitable medium.

(a)  

23.

It provides the appropriate biochemical and biophysical environment for microbial growth.

(a)  

24.

Indication of a positive growth for a liquid media.

(a)  

25.

Give an example of a semi-solid media.

(a)  

26.

Give an example of a NON – LIQUEFIABLE solid media.

(a)  

27.

Give an example of a General Purpose Media.

(a)  

28.

All of the following are enriched type culture media, except:

a)

BAP

b)

CAP

c)

Thayer Martin

d)

BHI

29.

This functional type culture media contains inhibitory agents that prevents / suppress growth of unwanted microbes.

(a)  

30.

This medium uses tomato juice as a selective agent.

(a)  

31.

It is a complete lysis of red cells in the media around and under the colonies: the area appears lightened (yellow) and transparent on BAP.

(a)  

32.

It is caused by hydrogen peroxide produced by the bacterium, oxidizing hemoglobin producing the green oxidized derivative methemoglobin resulting to an incomplete hemolysis on BAP.

(a)  

33.

This medium allows differentiation of xylose fermentation, lysine decarboxylation & hydrogen sulfide production.

(a)  

34.

All of the following conditions are the growth requirements of an anaerobic bacteria, except:

a)

Low oxygen content

b)

Reduced oxidation-reduction potential

c)

Extra nutrients

d)

None these

35.

This type of Transport Media has replaced glycerophosphate with balanced salt solution.

(a)  

36.

This type of Transport Media is recommended for fecal specimens suspicious for Campylobacter spp.

(a)  

37.

This functional type of culture media is used to monitor the effects of the administration of certain antimicrobial drugs.

(a)  

38.

This functional type culture media is used by industrial and environmental microbiologists to count the number of organisms in milk, water, food, soil and other samples.

(a)  

39.

All of the following are classifications of a tube distribution media, except:

a)

Butt

b)

Slant

c)

Slant/Butt

d)

Plated

40.

What is the temperature used for Deep-Freezing in preserving bacterial cultures?

(a)