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Downstreaming process

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

Chemically penicillin is _______

a)

6-Nitro Penicillanic acid

b)

6-amino penicillanic acid

c)

Beta lactam

d)

thiozolidine

2.

Which of the following is better fungus for penicillin production?

a)

Penicillium notatum

b)

Penicillium arborium

c)

Penicillium chrysogenum

d)

Streptomyces griseus

3.

Which of the following is essentially required in penicillin production?

a)

Glucose

b)

Potato dextose

c)

Barley

d)

Corn steep liquor

4.

Precipitation of proteins is carried out by _________

a)

Agarose

b)

Polyethylene glycol

c)

SDS

d)

Buffer

5.

Which of the following improves filtration?

a)

More cell mass

b)

Diatomaeceous earth

c)

Whatmann filter paper

d)

Aeration

6.

Which of the following filter is used to filter low solid content?

a)

Pressure leaf filter

b)

Rotary vacuum filter

c)

Plate and frame filter

d)

Filter can't be used

7.

When you are supplying broth for filtration, what do you think, which type of flow of broth is favored in rotary vacuum filter?

a)

Downward flow

b)

Upward flow

c)

Tangential flow

d)

radial flow

8.

Flocculation is not carried out by ________-

a)

Increasing hydrophilicity

b)

Decreasing hydrophilicity

c)

increasing hydrophobic nature

d)

neutralization of charges

9.

Osmotic shock favours _______

a)

aeration

b)

Cell flocculation

c)

Cell disruption

d)

Filtration

10.

separation of mycelia is carried out by_______

a)

Rotary vacuum filter

b)

Disc bowl centrifuge

c)

tubular bowl centrifuge

d)

Basket centrifuge

11.

Concentration of alcohol after extraction is 50mg/lit from broth with 1000mg/lit conc of alcohol. What will be distribution coefficient?

a)

20

b)

0.05

c)

5000

d)

Can't say

12.

Which extraction system is used to extract penicillin?

a)

Solid liquid extraction

b)

Whole broth processing

c)

Liquid liquid extraction

d)

Liquid soild extraction

13.

I want to separate IgG from broth, which method is suitable for extraction and purification

a)

use of filtration

b)

Use of ion exchange chromatography

c)

use of affinity chromatography

d)

Use of gel permeation chromatography

14.

Streptomycin is extracted from broth by ____

a)

Liquid liquid extraction

b)

Ion exchange resins

c)

Binding with cells

d)

simple filtartion

15.

How will you quantify the effect of antibiotic resistance

a)

Ditch plate technology

b)

Agar cup assay

c)

Serial dilution technique

d)

Enrichment in broth