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Protein synthesis aida

Total questions: 20

Worksheet time: 30mins

Name
Class
Date
1.

Which enzyme is required for DNA transcription?

a)

Helicase

b)

RNA polymerase

c)

DNA ligase

d)

DNA polymerase

2.

Gene mutations can occur by substitution, deletion or insertion. What is the smallest part of a DNA molecule that can be changed by a gene mutation?

a)

Base

b)

Codon

c)

Gene

d)

Nucleotide

3.

Which statements about mRNA are correct?

1) mRNA has base pairs.

2) mRNA can form hydrogen bonds.

3) mRNA is single-stranded.

a)

1,2 and 3

b)

1 and 2 only

c)

1 and 3 only

d)

2 and 3 only

4.

Which type of sugar and which type of bond are found in a DNA molecule?

a)

A

b)

B

c)

C

d)

D

5.

Which nucleic acid bases are purines?

a)

Adenine and Cytosine

b)

Cytosine and Thymine

c)

Guanine and Adenine

d)

Uracil and Cytosine

6.

In a genetic engineering experiment, a piece of double-stranded DNA containing 6000 nucleotides coding for a specific polypeptide is transcribed and translated. What is the total number of amino acids in this polypeptide?

a)

500

b)

1000

c)

2000

d)

3000

7.

Which statements about tRNA are correct?


1) Hydrogen bonds between bases temporarily hold tRNA against mRNA.

2) The base sequences in the tRNA molecules are the same as the base sequences in the mRNA that is being translated.

3) tRNA translates the base sequence in mRNA into the amino acid sequence in a protein.

a)

1,2 and 3

b)

1 and 2 only

c)

1 and 3 only

d)

2 and 3 only

8.

A section of a DNA molecule has the coding sequence: AGGCAATGGC. Which statements are correct?


1) This coding sequence contains 3 pyrimidines

2) The double-stranded DNA of this section contains 26 hydrogen bonds between complementary bases.

3) The double-stranded DNA of this section has 20 sugar-phosphate bonds.

a)

1,2 and 3

b)

1 and 2 only

c)

1 and 3 only

d)

2 and 3 only

9.

XNA is a laboratory-made nucleic acid made of nucleotides in which one component has been replaced by the chemical X. The chemical X is not found in nature. The part of the molecule responsible for coding is not changed.

Which organic component of a DNA or RNA nucleotide has been replaced by X?

a)

Five-carbon sugar

b)

Phosphate group

c)

Purine base

d)

Pyrimidine base

10.

The diagram shows the stages in the production of part of a polypeptide.


Which feature of the triplet code is illustrated by the information given?

a)

An amino acid can be coded for by more than one triplet.

b)

The triplet code is non-overlapping and is only read in one direction.

c)

The triplet code is universal for the DNA of all organisms.

d)

There are some triplets that code for ‘start’ and ‘stop’.

11.

The gene coding for glycogen synthase in muscle cells is known as GYS1. Explain what is meant by a gene. (2 marks)

4 lines
12.

There are a number of known mutations for GYS1. Outline how a mutation in GYS1 can lead to the formation of an altered polypeptide where one amino acid is replaced by a different amino acid. (3 marks)

4 lines
13.

More mRNA molecules than tRNA molecules are synthesised in cells. Suggest a reason for this. (1 mark)

4 lines
14.

Describe the role of ribosomes in protein synthesis.

(3 marks)

4 lines
15.

Name the nitrogenous bases, M and O.

a)

M - adenine O - cytosine

b)

M - uracil O - cytosine

c)

M - purine O - thymine

d)

M - adenine O - guanine

16.

Name the bonds shown by the dashed lines in Fig. 5.1.

a)

Glycosidic bond

b)

Hydrogen bond

c)

Ester bond

d)

Peptide bond

e)

Disulphide bond

17.

Insertion and deletion mutation is more harmful as they result in a ------ that changes the ordering of reading codons, therefore altering the entire ------ sequence that follows the mutation. On the other hand, substitution usually results in only a single amino acid being ------

(a)  

18.

In transcription, RNA that is formed is ------ stranded. One example of post-transcription modification is the addition of modified ------ to the 5' end of the RNA to form a 'cap'. Another is the addition of a protective tail composed of repeating ------ nucleotides.

(a)  

19.

The non-coding segments of genes, exons, are rejoined in a variety of different combinations in the post-translational stage

a)

True

b)

False

20.

tRNA contains U and not T in their anticodon loop

a)

TRUE

b)

FALSE