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WorksheetsRDNA WEEK 9- Plasmids and Vectors
Total questions: 28
Worksheet time: 19mins
How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?
1 - through the gene
2 - on either side of the gene
3 - through the gene and on either side of the gene
4 - twice on each side of the gene
What are selectable marker ?
a radium labelled marker
a type of sequence
resistant gene
a type of protein
LacZ gene is used to?
Differentiate between transformant and non transformant
Differentiate between clones with vector plasmids and without vector plasmids
Differentiate between competent cells and non competent cells
Differntiate between negative and false negative
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Insertional inactivation of β-galactosidase gene in recombinant bacteria
Inactivation of glycosidase gene in recombinant bacteria
Incompetence of recombinant bacteria containing β-galactosidase
insertional inactivation of α-galactosidase gene in non-recombinant bacteria
How do you overcome vector religation problem?
Use ligase enzyme
Use alkaline phosphatase enzyme
Use restriction enzymes
Use polymerase enzyme
First stage involved in gene cloning.
Amplification of the target gene
Insertion of the target gene
Polymerase Chain Reaction
Isolation of the target gene
The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.
Cloning
insertion
Transformation
Isolation
Types of plasmids produced after insertion of the target gene into a vector.
Recombinant plasmids
Non-recombinant plasmids
Bacterial plasmids
Isolated plasmids
Some of the steps involved in Gene Cloning are given below i) Insertion of isolated gene to the vector ii) Introduction of recombinant vector to the host iii) Isolation of desired gene iv) Expression of recombinant gene in host v) Extraction of recombinant gene product The correct sequence of steps involved are
1. iii, i, iv, ii, v
2. iii, i, ii, iv, v
3. i, ii, iii, iv, v
4. ii, i, iii, iv, v
An ideal plasmid to be used for recombinant DNA technology must have
minimum amount of DNA
relaxed replication control
all of these
DNA Ligase, used in recombinant DNA technology is obtained from
E.coli only
E.coli and also Ligase encoded by T4 phage
Saccharomyces
retroviruses
What is process P?
(a)
What is enzyme required for process P?
(a)
What is process Q?
(a)
What is the enzyme required for process Q?
(a)
What is molecule R?
(a)
What is process S?
(a)
What is process T?
(a)
What is the next step after process T?
isolation
cleave
insertion
transformation and amplification
screening
Which colonies are the desired result in gene cloning?
Blue colonies
White colonies
this diagram shows the action of...
terminal transferase
alkaline phosphatase
polynucleotide kinase
the klenow fragment
the capacity of the DNA fragment is 500 kb. Choose the ideal vector that can be used
BAC
pUC
Which organism's vectors are mostly used in molecular biology
a) Yeast
b) Mammalian cells
c) E.coli
d) Fungi
A plasmid can be considered as a suitable cloning vector if
A.it can be readily isolated from the cells
B.it possesses a single restriction site for one or more restriction enzymes
C.insertion of foreign DNA does not alter its replication properties
D.All of the above
What are the advantages of using E.coli in molecular biology
What are the disadvantages of using E.coli in molecular biology
