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Microscope Test 10/14/2022

Total questions: 24

Worksheet time: 48mins

Name
Class
Date
1.

A student is viewing a plant stem cross section using a compound light microscope.

What parts of the microscope should the student use to bring the image into focus?

a)

A and F

b)

B and E

c)

C and D

d)

D and F

2.

Which activity might lead to damage of a microscope and specimen?

a)

cleaning the ocular and objectives with lens paper

b)

focusing with low power first before moving the high power into position

c)

using the coarse adjustment to focus the specimen under high power

d)

adjusting the diaphragm to obtain more light under high power

3.

The diagram below shows how a coverslip should be lowered onto some single-celled organisms during the preparation of a wet mount.

Why is this a preferred procedure?

a)

The coverslip will prevent the slide from breaking.

b)

The organisms will be more evenly distributed.

c)

The possibility of breaking the coverslip is reduced.

d)

The possibility of trapping air bubbles is reduced.

4.

After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear

a)

larger and brighter

b)

smaller and brighter

c)

larger and darker

d)

smaller and darker

5.

The table below shows the position of slides of the letter "e" on the stages of four microscopes. The image of the "e" as seen using each microscope is also shown.

Which letters correctly identify the compound microscopes most likely used to provide the information in the table?

a)

A and D

b)

B and C

c)

C and D

d)

B and D

6.

A cell in the field of view of a compound light microscope is shown in the diagram below.

In which direction should the slide be moved to center the cell in the microscopic field?

a)

to the right and up

b)

to the right and down

c)

to the left and up

d)

to the left and down

7.

Which sequence contains the correct order of steps for a student to follow to observe the nucleus of protozoa in a stained wet mount, using a compound light microscope?

a)

1

b)

2

c)

3

d)

4

8.

Base your answer to the following question on the diagram of a compound light microscope below and on your knowledge of biology.

Which parts of this compound light microscope with clear lenses should be used to improve the quality of the image being observed in the high-power field of view?

a)

ocular and high-power objective

b)

diaphragm and fine-adjustment knob

c)

coarse-adjustment knob and low-power objective

d)

ocular and diaphragm

9.

A microscope with 10x and 40x objectives is used to scan a slide to locate a specimen. The largest field of vision would be provided by using the

a)

coarse adjustment

b)

widest diaphragm opening

c)

10x objective

d)

40x objective

10.

The diagram below represents a hydra as viewed with a compound light microscope.

If the hydra moves toward the right of the slide preparation, which diagram best represents what will be observed through the microscope?

a)

b)

c)

d)

11.

The size of the image of a cell being examined with a microscope is determined by the

a)

light source and fine adjustment

b)

stage and stage clips

c)

objectives and ocular

d)

diaphragm and coarse adjustment

12.

Base your answer to the following question on the diagrams below and on your knowledge of biology.

Which microscope setup would allow a student to see the largest field?

a)

A

b)

B

c)

C

d)

D

13.

The diagram at the right represents a strand of algae

viewed under the low-power objective of this

microscope. Under the high-power objective, how

would this same slide appear?

a)

b)

c)

d)

14.

A specimen was viewed under the high-power objective of a compound light microscope. Its length was estimated to be 0.75 millimeter. What is the approximate length of the specimen in micrometers?

(HINT: K H D B D C M _ _ u)

a)

0.00075

b)

0.75

c)

75

d)

750

15.

The diagram below represents the letter "h" as seen in the low-power field of view of a compound light microscope.

Which diagram best represents the field of view if the slide is not moved and the objective is switched to high power?

a)

b)

c)

d)

16.

Base your answer to the following question on the diagram below of a single-celled organism observed by a student using the low-power objective of a microscope.

How should the student move the slide on the stage to center the single-celled organism in the field?

a)

away from herself and to her right

b)

away from herself and to her left

c)

toward herself and to her right

d)

toward herself and to her left

17.

Which part of a microscope should be used with the

low-power objective, but not with the high-power

objective?

a)

coarse adjustment

b)

fine adjustment

c)

diaphragm

d)

ocular

18.

Which part of a light microscope would most likely be damaged if the coarse adjustment is improperly used while a specimen is being observed under high power?

a)

objective lens

b)

light source

c)

iris diaphragm

d)

eyepiece lens

19.

A student using a compound microscope measured the diameter of several red blood cells and found that the average cell length was 8 micrometers. What is the average length of a single red blood cell in millimeters?

a)

.08

b)

0.008

c)

80

d)

800

20.

A laboratory technique is illustrated in the diagram below.

The technique of lowering the cover slip at an angle is used to

a)

make organelles more visible

b)

reduce the formation of air bubbles

c)

make the specimen transparent

d)

reduce the size of the specimen

21.

Which laboratory procedure is represented in the diagram below?

a)

placing a coverslip over a specimen

b)

removing a coverslip from a slide

c)

adding stain to a slide without removing the coverslip

d)

reducing the size of air bubbles under a cover-slip

22.

A student observing a specimen using the low-power objective of a compound light microscope has difficulty viewing the image because the field of view is too dark. The student can correct the problem by

a)

adjusting the diaphragm

b)

using the coarse adjustment

c)

switching to the high-power objective

d)

cleaning the high-power objective

23.

Two views of the same onion epidermal cells, as seen with a compound light microscope, are shown in the diagram below.

What was most likely done to change the view from A to B?

a)

A stain (Lugol's iodine solution) was added to the cells.

b)

The 40x objective was switched to the 10x objective.

c)

The IOx objective was switched to the 40x objective.

d)

Salt water was added to the cells.

24.

To locate a specimen on a prepared slide with a compound microscope, a student should begin with the low-power objective rather than the high-power objective because the

a)

field of vision is smaller under high power than under low power

b)

field of vision is smaller under low power than under high power

c)

specimen does not need to be stained for observation under low power but must be stained for observation under high power

d)

portion of the specimen that can be observed under low power is less than the portion that can be observed under high power