WorksheetsMicroscope Test 10/14/2022
Total questions: 24
Worksheet time: 48mins
A student is viewing a plant stem cross section using a compound light microscope.
What parts of the microscope should the student use to bring the image into focus?
A and F
B and E
C and D
D and F
Which activity might lead to damage of a microscope and specimen?
cleaning the ocular and objectives with lens paper
focusing with low power first before moving the high power into position
using the coarse adjustment to focus the specimen under high power
adjusting the diaphragm to obtain more light under high power
The diagram below shows how a coverslip should be lowered onto some single-celled organisms during the preparation of a wet mount.
Why is this a preferred procedure?
The coverslip will prevent the slide from breaking.
The organisms will be more evenly distributed.
The possibility of breaking the coverslip is reduced.
The possibility of trapping air bubbles is reduced.
After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear
larger and brighter
smaller and brighter
larger and darker
smaller and darker
The table below shows the position of slides of the letter "e" on the stages of four microscopes. The image of the "e" as seen using each microscope is also shown.
Which letters correctly identify the compound microscopes most likely used to provide the information in the table?
A and D
B and C
C and D
B and D
A cell in the field of view of a compound light microscope is shown in the diagram below.
In which direction should the slide be moved to center the cell in the microscopic field?
to the right and up
to the right and down
to the left and up
to the left and down
Which sequence contains the correct order of steps for a student to follow to observe the nucleus of protozoa in a stained wet mount, using a compound light microscope?
1
2
3
4
Base your answer to the following question on the diagram of a compound light microscope below and on your knowledge of biology.
Which parts of this compound light microscope with clear lenses should be used to improve the quality of the image being observed in the high-power field of view?
ocular and high-power objective
diaphragm and fine-adjustment knob
coarse-adjustment knob and low-power objective
ocular and diaphragm
A microscope with 10x and 40x objectives is used to scan a slide to locate a specimen. The largest field of vision would be provided by using the
coarse adjustment
widest diaphragm opening
10x objective
40x objective
The diagram below represents a hydra as viewed with a compound light microscope.
If the hydra moves toward the right of the slide preparation, which diagram best represents what will be observed through the microscope?
The size of the image of a cell being examined with a microscope is determined by the
light source and fine adjustment
stage and stage clips
objectives and ocular
diaphragm and coarse adjustment
Base your answer to the following question on the diagrams below and on your knowledge of biology.
Which microscope setup would allow a student to see the largest field?
A
B
C
D
The diagram at the right represents a strand of algae
viewed under the low-power objective of this
microscope. Under the high-power objective, how
would this same slide appear?
A specimen was viewed under the high-power objective of a compound light microscope. Its length was estimated to be 0.75 millimeter. What is the approximate length of the specimen in micrometers?
(HINT: K H D B D C M _ _ u)
0.00075
0.75
75
750
The diagram below represents the letter "h" as seen in the low-power field of view of a compound light microscope.
Which diagram best represents the field of view if the slide is not moved and the objective is switched to high power?
Base your answer to the following question on the diagram below of a single-celled organism observed by a student using the low-power objective of a microscope.
How should the student move the slide on the stage to center the single-celled organism in the field?
away from herself and to her right
away from herself and to her left
toward herself and to her right
toward herself and to her left
Which part of a microscope should be used with the
low-power objective, but not with the high-power
objective?
coarse adjustment
fine adjustment
diaphragm
ocular
Which part of a light microscope would most likely be damaged if the coarse adjustment is improperly used while a specimen is being observed under high power?
objective lens
light source
iris diaphragm
eyepiece lens
A student using a compound microscope measured the diameter of several red blood cells and found that the average cell length was 8 micrometers. What is the average length of a single red blood cell in millimeters?
.08
0.008
80
800
A laboratory technique is illustrated in the diagram below.
The technique of lowering the cover slip at an angle is used to
make organelles more visible
reduce the formation of air bubbles
make the specimen transparent
reduce the size of the specimen
Which laboratory procedure is represented in the diagram below?
placing a coverslip over a specimen
removing a coverslip from a slide
adding stain to a slide without removing the coverslip
reducing the size of air bubbles under a cover-slip
A student observing a specimen using the low-power objective of a compound light microscope has difficulty viewing the image because the field of view is too dark. The student can correct the problem by
adjusting the diaphragm
using the coarse adjustment
switching to the high-power objective
cleaning the high-power objective
Two views of the same onion epidermal cells, as seen with a compound light microscope, are shown in the diagram below.
What was most likely done to change the view from A to B?
A stain (Lugol's iodine solution) was added to the cells.
The 40x objective was switched to the 10x objective.
The IOx objective was switched to the 40x objective.
Salt water was added to the cells.
To locate a specimen on a prepared slide with a compound microscope, a student should begin with the low-power objective rather than the high-power objective because the
field of vision is smaller under high power than under low power
field of vision is smaller under low power than under high power
specimen does not need to be stained for observation under low power but must be stained for observation under high power
portion of the specimen that can be observed under low power is less than the portion that can be observed under high power
