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WorksheetsBIOL 2420 Lab Exam 1 Review
Total questions: 24
Worksheet time: 15mins
How long should you wash your hands for (minimum)?
10 minutes
1 minute
20 seconds
15 minutes
Where and how do gloves get disposed of at the end of lab?
regular trash
biohazard
biohazard wrapped in paper towel
glass disposal
Where do we store cultures when we don't want them to grow further?
37 degrees C
4 degrees C
25 degrees C
60 degrees C
Identify the indicated part of the microscope
ocular lens
objective lens
stage
diaphragm
Calculate the resolving power of the 40X lens (NA = 0.65)
220 nm
1100 nm
423 nm
2750 nm
Identify the cell morphology
pallisades
tetrad
streptobacilli
diplococcus
Identify the cell structure
endospores
flagella
capsules
fimbriae
What should be done next after transferring inoculum from a loop onto a slant and removing the loop from the tube?
replace cap
remove cap
sterilize loop
sterilize lip of tube
If a broth culture has growth floating on the surface, it's broth morphology would be:
flocculant
turbid
pellicle
sediment
If a colony was red, and the pigment did not spread into the agar, it's pigment would be described as:
negative, non-pigmented
positive, water insoluble
positive, water soluble
negative, water soluble
If a colony had wavy edges, its margin would be described as
undulate
entire
umbonate
irregular
Crystal violet is a(n)
anionic dye
cationic dye
non-ionic dye
A mixed culture of cells would stain ________ in a simple stain.
pink and purple
red and blue
the same color
fuschia
Alcohol (decolorizer) contracts the peptidoglycan layer in
Gram-positive cells
Gram-negative cells
mycobacteria
If a student forgot to add the crystal violet to the Gram stain, what would be the result?
all cells would be colorless
all cells would be purple
cells would be a mix of pink and purple
all cells would be pink
The primary stain in the capsular stain is
crystal violet
india ink
carbolfuchsin
safranin
The final dilution of a 10-1 dilution and a 10-2 dilution would be:
10-1
10-2
10-3
10-4
What is the CFU per ml if 142 CFUs were counted, 1 mL was plated, and the dilution was 10-4?
142 x 10-4
1.42 x 10-6
1.42 x 106
1.42 x 104
What category of chemical agent does the antiseptic povidone belong to?
halogen
alcohol
phenolic
aldehyde
What is the mechanism of action of the antibiotic Bactrim?
inhibits protein synthesis
inhibits nucleic acid synthesis
inhibits cell wall synthesis
inhibits metabolism
When determining if an antibiotic is useful, the one that produced the largest zone of inhibition should always be chosen.
True
False
Which group of bacteria are catalase positive?
streptococci
entero-bacteriaceae
staphylococci
non-
entero-
bacterieaceae
When oxidase reagent is purple, it is
oxidized
reduced
What is the reagent used in the catalase test?
catalase
oxidase
hydrogen peroxide
phenylenediamene
