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BIOL 2420 Lab Exam 1 Review

Total questions: 24

Worksheet time: 15mins

Name
Class
Date
1.

How long should you wash your hands for (minimum)?

a)

10 minutes

b)

1 minute

c)

20 seconds

d)

15 minutes

2.

Where and how do gloves get disposed of at the end of lab?

a)

regular trash

b)

biohazard

c)

biohazard wrapped in paper towel

d)

glass disposal

3.

Where do we store cultures when we don't want them to grow further?

a)

37 degrees C

b)

4 degrees C

c)

25 degrees C

d)

60 degrees C

4.

Identify the indicated part of the microscope

a)

ocular lens

b)

objective lens

c)

stage

d)

diaphragm

5.

Calculate the resolving power of the 40X lens (NA = 0.65)

a)

220 nm

b)

1100 nm

c)

423 nm

d)

2750 nm

6.

Identify the cell morphology

a)

pallisades

b)

tetrad

c)

streptobacilli

d)

diplococcus

7.

Identify the cell structure

a)

endospores

b)

flagella

c)

capsules

d)

fimbriae

8.

What should be done next after transferring inoculum from a loop onto a slant and removing the loop from the tube?

a)

replace cap

b)

remove cap

c)

sterilize loop

d)

sterilize lip of tube

9.

If a broth culture has growth floating on the surface, it's broth morphology would be:

a)

flocculant

b)

turbid

c)

pellicle

d)

sediment

10.

If a colony was red, and the pigment did not spread into the agar, it's pigment would be described as:

a)

negative, non-pigmented

b)

positive, water insoluble

c)

positive, water soluble

d)

negative, water soluble

11.

If a colony had wavy edges, its margin would be described as

a)

undulate

b)

entire

c)

umbonate

d)

irregular

12.

Crystal violet is a(n)

a)

anionic dye

b)

cationic dye

c)

non-ionic dye

13.

A mixed culture of cells would stain ________ in a simple stain.

a)

pink and purple

b)

red and blue

c)

the same color

d)

fuschia

14.

Alcohol (decolorizer) contracts the peptidoglycan layer in

a)

Gram-positive cells

b)

Gram-negative cells

c)

mycobacteria

15.

If a student forgot to add the crystal violet to the Gram stain, what would be the result?

a)

all cells would be colorless

b)

all cells would be purple

c)

cells would be a mix of pink and purple

d)

all cells would be pink

16.

The primary stain in the capsular stain is

a)

crystal violet

b)

india ink

c)

carbolfuchsin

d)

safranin

17.

The final dilution of a 10-1 dilution and a 10-2 dilution would be:

a)

10-1

b)

10-2

c)

10-3

d)

10-4

18.

What is the CFU per ml if 142 CFUs were counted, 1 mL was plated, and the dilution was 10-4?

a)

142 x 10-4

b)

1.42 x 10-6

c)

1.42 x 106

d)

1.42 x 104

19.

What category of chemical agent does the antiseptic povidone belong to?

a)

halogen

b)

alcohol

c)

phenolic

d)

aldehyde

20.

What is the mechanism of action of the antibiotic Bactrim?

a)

inhibits protein synthesis

b)

inhibits nucleic acid synthesis

c)

inhibits cell wall synthesis

d)

inhibits metabolism

21.

When determining if an antibiotic is useful, the one that produced the largest zone of inhibition should always be chosen.

a)

True

b)

False

22.

Which group of bacteria are catalase positive?

a)

streptococci

b)

entero-bacteriaceae

c)

staphylococci

d)

non-

entero-

bacterieaceae

23.

When oxidase reagent is purple, it is

a)

oxidized

b)

reduced

24.

What is the reagent used in the catalase test?

a)

catalase

b)

oxidase

c)

hydrogen peroxide

d)

phenylenediamene