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Micro 3

Total questions: 20

Worksheet time: 12mins

Name
Class
Date
1.

Which of the following is the best definition of respiration?

a)

a sequence of carrier molecules with the final electron acceptor from the environment

b)

a method of generating ATP

c)

a series of reactions in which pyruvic acid is oxidized to CO2 and H2O

d)

the complete oxidation of glucose to CO2 and H2O

2.

This inhibition mechanism does not bind to the active site of the enzyme.

(a)  

3.

All microorganims are heterotrophs.

a)

True

b)

False

4.

An apoenzyme is where the _______________ is located

a)

cofactor

b)

redox reaction 

c)

coenzyme 

d)

active site

5.

An enzyme 

a)

becomes part of the final products 

b)

is heat and pH labile

c)

is nonspecific for substrate 

d)

is consumed by the reaction

6.

Assume you inoculated 100 facultatively anaerobic cells onto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species onto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should have 

a)

more colonies on the aerobic plate. 

b)

more colonies on the anaerobic plate.

c)

the same number of colonies on both plates

d)

no growth in the anaerobic plate

7.

Which of the following processes does not generate ATP? 

a)

oxidative phosphorylation

b)

substrate-level phosphorylation

c)

the Calvin-Benson cycle

d)

photophosphorylation

8.

Which of the following compounds has the greatest amount of energy for a cell?

a)

glucose

b)

CO2 

c)

ATP 

d)

lactic acid

9.

The time required for a cell to undergo binary fission is called the 

a)

generation time 

b)

lag period

c)

growth curve 

d)

exponential growth rate 

10.

Which culture uses the most glucose?

a)

E. coli growing in glucose broth at 35°C with O2 for 5 days

b)

E. coli growing in glucose broth, supplemented with NO3- , at 35°C with O2 for 5 days

c)

E. coli growing in glucose broth, supplemented with NO3- , at 35°C without O2 for 5 days

d)

E. coli growing in glucose broth at 35°C without O2 for 5 days

11.

Which culture produces the most ATP?

a)

E. coli growing in glucose broth at 35°C with O2 for 5 days

b)

E. coli growing in glucose broth, supplemented with NO3- , at 35°C without O2 for 5 days

c)

E. coli growing in glucose broth at 35°C without O2 for 5 days

d)

E. coli growing in glucose broth, supplemented with NO3- , at 35°C with O2 for 5 days

12.

In a viable plate count, each _____________ represents a ______________ from the sample population. 

a)

hour, generation

b)

cell, generation

c)

colony, cell 

d)

cell, colony 

13.

Turbidometry, by means of spectrophotometry, is a method to determine the EXACT number of cells in our culture.

a)

No

b)

Yes

14.

Which of the following types of media would not be used to culture aerobes? 

a)

differential media

b)

selective media 

c)

reducing media 

d)

enrichment media

15.

Where do photosynthetic organism, e.g. cyanobacteria, obtain their energy from?

(a)  

16.

Active transport of a substance across a membrane requires 

a)

the expenditure of ATP 

b)

water

c)

a gradient 

d)

diffusion

17.

Psychrophiles would be expected to grow 

a)

at low pH

b)

on the human body 

c)

in hot springs 

d)

at refrigeration temperatures 

18.

Autotrophs use (a)   as a source of carbon.

19.

The stage in population growth with the highest rate of cell division is the 

a)

exponential phase 

b)

lag phase 

c)

stationary phase 

d)

enlargement phase

20.

An enzyme the activation energy required for a chemical reaction. 

a)

increases 

b)

catalyzes

c)

lowers 

d)

converts