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WorksheetsModule 9: Oxidative Phosphorylation
Total questions: 26
Worksheet time: 24mins
Which is not a reduced fuel for the cell?
Carbohydrates
Lipids
Amino Acids
Enzymes
What are the reduced cofactors that electrons from reduced cells get transferred to?
NAD+
FADH2
NADH
FAD+
In _ energy from NADH and FADH2 is used to make ATP.
(a)
What provides energy needed for ADP phosphorylation?
The energy released by electron transport is used to transport protons ___ the electrochemical gradient.
With
Across
Against
Outside
For ATP synthesis, the membrane must...
Contain proteins that couple the "downhill" flow of electrons in the ETC with the "uphill" flow of protons across the membrane.
Contain proteins that couple the "uphill" flow of electrons in the ETC with the "downhill" flow of protons across the membrane.
Contain a protein that couples the "downhill" flow of protons to the phosphorylation of ADP.
Contain a protein that couples the "uphill" flow of protons to the phosphorylation of ADP.
The inner membrane of mitochondria are relatively impermeable and do NOT contain:
Complexes
ADP-ATP translocase
ATP synthase (F0 and F1)
Other membrane transporters
Citric Acid Cycle Enzymes
Cytochromes and Iron-Sulfer Clusters are both
Single-electron carriers
coordinated by cysteines in the protein
lipid soluble and accept electrons
Mobile electron carriers
Upon accepting 2 electrons, Ubiquinone picks up _ protons to become ubiquinol.
1
5
3
2
Coenzyme Q transports electrons from complexes 1 and 2 to_
Complex 4
Across the membrane
Outside the membrane
complex 3
For Complex 1: NADH: Oxidoreductase, how many electrons are accepted from NADH and how many protons are accepted per NADH. Reduced coenzyme Q picks up how many protons?
2, 4, 2
4, 2, 2
2, 2, 2
2, 2, 4
For Complex 2: Succinate Dehydrogenase, How many electrons does FAD accept from succinate. And what is the end result. Is this complex a proton pump? Why or why not? Lastly what is the role of succinate dehydrogenase?
How many protons are transported across the membrane per two electrons that reach cyt c? How many of the protons come from QH2. What is the net transfer of protons per reduced enzyme Q?
4, 2, 4
2, 4, 4
4,4,4
2, 2, 4
Ubiquinone moves through the _ while Cytochrome C moves through the _.
intermembrane space; membrane
cytosol ; matrix
membrane; intermembrane space
matrix; innermembrane
Cytochrome c carries _ from cytochrome bc1 complex to cytochrome oxidase.
(a)
What method can correct free radical production?
Increased glutathione
Removing protons from the matrix
Decreased glutathione
More electrons
Which is NOT a means by which the ETC created the electrochemical proton gradient?
Actively transporting protons across the membrane.
Chemically removing protons from the matrix.
Releasing protons into the intermembrane space
Removing protons from the intermembrane space.
_ sets up a proton motive force.
(a)
Energy of proton-motive force drives synthesis of_?
(a)
ATP synthesis does not require electron transport. Electron transport requires ATP synthesis.
True
False
Cyanide (CN-), Venturicidin, and Oligomycin all catalyze ATP synthase while Dinitrophenol inhibits it.
True
False
In mitochondrial ATP Synthase Complex, which one Catalyzes Hydrolysis of ATP?
F1
F0
What are the three conformations of the ATP synthase complex?
The conformational change in one of the three pairs promotes hydrolysis of ADP and Pi into ATP. What causes this?
Proton translocation
Electron transfer
Cell signals
Matrix changing
What is the solution(s) to the issue of what is preventing NADH from entering the ETC in eukaryotic organisms?
Malate Aspartate shuttle
Cytosol Transport
Glycerol-3-phosphate shuttle
Proton transport
UCP1 is an uncoupling protein that produces heat.
True
False
