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Nucleic acid L3

Total questions: 10

Worksheet time: 13mins

Name
Class
Date
1.

DNA denaturation can not be done by

a)

Heat

b)

Sound wave

c)

change in H+ ion concentration in solution

d)

Chaotropic agents

2.

Which enzyme can denature DNA intracelluarly?

a)

Helicase

b)

Denaturase

c)

Nucleotidase

d)

All of the above

3.

What is hyperchromic shift?

4 lines
4.

You have two DNA samples with sequence repeats of the following

Sample 1: ATGTTAGCCAAT

Sample 2: ATGGGCCTAGCG

Which of the two samples has higher melting temperature. Explain why.

4 lines
5.

DNA helix in eukaryotic cell is wound around histone octamer. This is an example of_________

a)

Toroid supercoilng

b)

Plectonemic supercoiling

c)

Positive supercoiling

d)

None of the above

6.

Which of these is/are a topological property of DNA

a)

Linking number

b)

Writhe

c)

Twist

d)

All of the above

7.

Linking number in the this DNA double strand picture is ____________

a)

6

b)

12

c)

18

d)

Can't be determined

8.

DNA topoisomearse was discovered by

a)

Joshua Lederberg

b)

Fred Griffith

c)

J.C. Wang

d)

Oswald Avery

9.

Make a DNA sequence which is pallindromic in nature.

4 lines
10.

G-quardruplex structure is observed in

a)

Centromeric region of chromosome

b)

Telomeric region of chromosome

c)

Pericentromeric region of chromosome

d)

Nucleolus region