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AP Bio - Biotechnology Quiz

Total questions: 13

Worksheet time: 7mins

Name
Class
Date
1.

Which of the following is true about a positive control?

a)

A known positive result

b)

A safeguard against a false positive

c)

A safeguard against a false negative

d)

Included in diagnostic tests

2.

Which of the following is true about a negative control? (choose two)

a)

A known negative result

b)

A safeguard against a false positive

c)

A safeguard against a false negative

d)

No outcome is expected

3.

The independent variable of an experiment:

a)

Is something you can change

b)

Is something you can control

c)

Affects the dependent variable

d)

All of these answers are correct

4.

The dependent variable of an experiment:

a)

Fluctuates with changes in the independent variable

b)

Is something you can control

c)

Affects the independent variable

d)

All of these answers are correct

5.

If your hypothesis is incorrect, your experiment is a failure.

a)

TRUE

b)

FALSE

6.

Variables are the conditions, aspects, or components that are changeable and

controllable during an experiment.

a)

TRUE

b)

FALSE

7.

What specific technique is this biotechnology used for?

(a)  

8.

Once loaded into the well, DNA will...

a)

move randomly throughout the agarose gel.

b)

be pushed away from the positive end end.

c)

move towards the positive electrode once an electric current is running through.

d)

move towards the negative electrode once an electric current is running through.

9.

The diagram illustrates the results of electrophoresis of DNA sequences obtained from a family of two adults and three children. Results for another female (X) are included for comparison. The banding patterns of the DNA fragments reveal that

a)

child 1 and child 2 cannot be biological siblings

b)

child 1 and child 3 probably look like the mother

c)

the mother cannot be the biological parent of all three children

d)

the mother’s DNA has the same DNA sequence as the father’s DNA

10.

Which of the following is the best explanation for the fragment pattern for individual X ?

a)

She has only one member of this chromosome pair.

b)

She has only one living parent.

c)

She is homozygous for this particular DNA fragment.

d)

She is the mother’s child but has a different father than the other children.

11.

In a transformation experiment, a sample of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to a second sample. Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. The shaded area represents extensive growth of bacteria; dots represent individual colonies of bacteria.

Plates that have only ampicillin-resistant bacteria growing include which of the following?

a)

I only

b)

III only

c)

IV only

d)

I and III

12.

Which of the following best explains why there is no growth on plate II?

a)

The initial E. coli culture was not ampicillin-resistant.

b)

The transformation procedure killed the bacteria.

c)

Nutrient agar inhibits E. coli growth.

d)

The bacteria on the plate were transformed.

13.

Which of the following statements best explains why there are fewer colonies on plate IV than on plate III?

a)

Plate IV is the positive control.

b)

Not all E. coli cells are successfully transformed.

c)

The bacteria on plate III did not mutate.

d)

The plasmid inhibits E. coli growth.