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WorksheetsLabelling and fixation (histopathology)
Total questions: 24
Worksheet time: 12mins
In histology procedure, once the tissue has been selected for processing, what is the next step?
trimming
labeling
embedding of tissue
sectioning
Is it true, after biopsy or specimen collection, they must be labelled and bear the same number in all stages of procedure.
yes
no
By using which of the following materials can the labeling be done in the histological procedure?
graphite pencil-led typewritten
india ink written label
printed
all of the above
Can ordinary ink be used in labeling the specimen during the histological procedure?
yes
no
In histological procedures, labeling with SK (stock kept) at the end of grossing notes are written for which type of specimen?
no tissue is left or no remainings left from the specimen
remainings of all specimens and are preserved in formalin
What is the primary function of fixatives?
preserve cell structure
damages the specimen
increases autolysis
sectioning of specimen
The process in which, there is degradation of cellular structure due to degenerative enzymes from excised tissue itself is called?
necrosis
autolysis
autopsy
all of the above
What is the other use of fixative in histology?
allowing easy handling of specimen during processing
to protect and harden the tissue
increases autolysis
both A and B
What amount (volume) of fixative should be used for the specimen (tissue)?
5 times more fixatives than the tissue volume
20 times more fixatives than the tissue volume
10 times more fixatives than the tissue volume
50 times more fixatives than the tissue volume
Which types of samples take longer hours to reach deeper tissue?
larger samples
smaller samples
it does not depend on the size
none of these
What must be considered while using the fixative to diffuse through the tissue to fix?
tissue size
tissue density
type of fixative
all of the above
What are the types of fixatives used in histological procedure?
fast and slow fixatives
active and non-active fixatives
simple and compound fixatives
all of the above
Which type of fixative contains one chemical substance?
compound fixatives
simple fixatives
fast fixatives
slow fixatives
Which is the most common type of simple fixative used in histology?
mercuric chloride
acetone
formaline
formic acid
Which type of fixative is carnoy's fluid?
cytoplasmic
nuclear
histochemical
microanatomical
Which of the following type of compound fixative is not of microanatomical type?
neutral buffered formaline
formal saline
carnoy's solution
zenker's fluid
What amount of formalin is used to fix the tissue/specimen during histology pocessing?
20%
10%
2%
12%
Which fixative colors tissue yellow during fixation in histological procedure?
picric acid
glutaraldehyde
formaline
absolute alcohol
Which fixative is used to preserve glycogen?
absolute alcohol
picric acid
both A and B
none of these
Which type of simple fixative stain more brilliant, penetrates poorly and produces shrinkage of tissues?
picric acid
formaline
mercuric chloride
absolute alcohol
Which fixative is the choice in electron microscopy?
picric acid
mercuric chloride
formaline
glutaraldehyde
What is the composition of carnoy's solution?
chloroform
absolute alcohol
glacial acetic acid
all of the above
Which of the following is true about carnoy's solution?
it is used for urgent biopsy specimens
it gives excellent nuclear fixation and preserves glycogen
specimens fixed in carnoy's solution should be directly transferred to 95% or absolute alcohol
all of the above
Which of the following is not true about the bouin solution?
it results in sharp hemtoxylin and eosin staining
it causes decreased sensitivity of immunohistochemical tests
it is not preferred by any pathologist
it causes increased degradation of DNA and RNA by picric acid
