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WorksheetsBIOL1012 Practical 1
Total questions: 15
Worksheet time: 5mins
Gel electrophoresis separates molecular products based on:
Shape
Size
Charge
Evolutionary similarity
When traveling through an agarose gel, larger molecular products will migrate _________ smaller molecular products
Faster than
Slower than
At the same rate
Buffer is used instead of water when making and running gels. Which of the following is NOT true for buffer?
Buffer enhances the transmission of electric currents in water
Buffer is more homogeneous than water
Buffer neutralizes the charge on molecular products prior to electrophoresis
Buffer neutralizes the charge on molecular products prior to electrophoresis
The size of the molecular products is determined by
The intensity of the band
The percentage of agarose gel
Comparison with a molecular weight “ladder”
Being familiar with your gel and “eyeballing” it
In a laboratory, the following should not be worn
loose clothing
dangling jewelry
sandals.
All options here
The following footwear is best in the laboratory
sandals
open-toed shoes
closed-toed shoes
shoes appropriate for the weather
Which of the following lens objective would you use to obseve pathological samples in great details ?
X4
X10
X40
How is the image under the microscope compared to image through normal eyes ?
Backward and upright
Backward and upside down
Upside down only
No differences
How does centrifuge separate a heterogenous mixture?
Spinning the fluid at high speed
Spinning the fluid at low speed
Standing still under low pressure
Standing still under low temperature
Which of the following is NOT allowed/recommended when operating the centrifuge?
All tubes/containers are properly balanced in the rotor
Any tubes/containers can be used in the centrifuge
The centrifuge lid should be closed during operation
All tubes/containers should not be overfilled
Which objective lens should you begin with when examining a slide?
The highest magnification lens.
The lowest magnification lens.
Any lens will do, it doesn't matter.
The lens with the largest diameter.
How should you adjust the focus when using the high-power objective lens?
Use only the coarse focus knob.
Use only the fine focus knob.
Use the coarse focus knob first, then fine-tune with the fine focus knob.
Focus by moving the slide up and down.
When carrying a microscope, which of the following is the safest method?
Holding the arm with one hand.
Grasping the base with one hand.
Holding the arm with one hand and supporting the base with the other
Balancing the microscope on your shoulder.
What should you never do with the lenses of a microscope?
Clean them with a soft, clean cloth.
Use lens cleaner designed for microscopes.
Touch them with your fingers.
Wipe them with a paper designed for microscopes.
5. What is the most crucial step before using a centrifuge?
Turning on the machine and setting the desired speed.
Ensuring the tubes are balanced evenly within the rotor.
Adding the samples to the tubes and securing the lids.
Checking the expiration date of the centrifuge tubes
