The following data are for a liquid chromatographic column with a chromatogram of a mixture of species A, B, C, and D: 

Calculate for species B and C the selectivity factor α (three significant figures)

(a)  

The following data are for a liquid chromatographic column with a chromatogram of a mixture of species A, B, C, and D: 

Calculate for species B and C the average plate height in mm (three significant figures)

(a)  

The following data are for a liquid chromatographic column with a chromatogram of a mixture of species A, B, C, and D: 

Calculate for species B and C the length of column (cm) necessary to separate the two species with a resolution of 2,5. (three significant figures)

(a)  

The following data are for a liquid chromatographic column with a chromatogram of a mixture of species A, B, C, and D: 

Calculate for species B and C the time required (min) to separate the two species on the column with a resolution of 2,5. (three significant figures). It should be done according to the retention time of the most retained component.

(a)  

How many times is the resolution affected when doubling the length of the chromatographic column?

How can we decrease the theoretical plate height in HPLC? Explain.

If I work in reverse phase, what happens to the retention factor when increasing the polarity of the mobile phase? Justify.

If I work in reverse phase and have analytes with acidic groups, how can I increase the retention of the compounds? Justify.

How does temperature affect the chromatographic parameters in HPLC? Explain.