NEW
Font size
WorksheetsMICROBIO QUIZ #1
Total questions: 20
Worksheet time: 13mins
Randy, a college student, was experiencing sore throat and high fever for 3 days. He was diagnosed with Strep throat which is caused by the pathogen Streptococcus pyogenes. He was prescribed with Amoxicillin for 1 week. After the treatment was completed, his symptoms resolved. He was currently taking microbiology and has recently learned that streptococci are gram-positive bacteria. What do you think is the mechanism how the antibiotic worked for his condition?
Streptococci have cell walls made up of thick peptidoglycan and the antibiotic disrupted the bacteria’s cell wall synthesis.
Streptococci have cell wall made up of a waxy substance called mycolic acid which was easily destroyed by the antibiotic.
Streptococci do not contain cell wall that is why the antibiotic worked on the bacteria.
The bacteria have outer membrane that contains lipopolysaccharide.
Which of the following is NOT involved in the DNA transcription in bacteria?
DNA template
mRNA
tRNA
DNA polymerase
Term used to describe bacteria with tuft of flagella at one end.
lophotrichous
amphitrichous
peritrichous
atrichous
This bacterial structure is made up of polysaccharide that functions to prevent phagocytosis and thus contribute to its virulence.
capsule
spore
plasmid
metachromatic granules
This is the part in the bacterial cell which is the site of almost all metabolic processes.
cell membrane
cytoplasm
mitochondrion
ribosome
Raw food can still harbor resistant microorganisms even if it was allowed to boil for 30 minutes because of the presence of this structure.
endospore
flagella
pili
cell wall
This is the metabolic pathway in the cell that generates the greatest amount of ATP.
Electron transport chain
Pentose Phosphate Pathway
Embden-Meyerhof-Parnas Pathway
Kreb’s cycle
Under the 5-Kingdom System of Classification, what domain are bacteria classified?
prokaryotae
protista
animalia
fungi
A 50-year old man was admitted to the hospital due to respiratory distress which started with fever, non-productive cough and easy fatigability 4 days ago. On gram staining, purple, rod-shaped bacteria arranged in chains were seen and later on identified as Bacillus anthracis. These descriptions about the bacteria are examples of
phenotypic characteristics
genotypic characteristics
physiologic characteristics
binomial system of nomenclature
Which of the following is TRUE regarding prokaryotes?
The number of mitochondria in a cell varies greatly depending on the activities required of that cell.
Bacteria’s cell membranes are enclosed by envelopes.
Prokaryotes either contain DNA or RNA.
Plasmids are linear molecules of double-stranded DNA that can be found in bacteria.
Which of the following equipment in the microbiology lab does not use the principle of dry heat of sterilization?
autoclave
hot air oven
incinerator
Bunsen burner
These are instruments used in the microbiology lab to transfer microbial growth from one container to another.
inoculating loop and needle
Petri dishes
densitometer
incubator
These are the minimum standard parameters that should be met to assure proper sterilization with an autoclave.
temperature should reach 121 degrees Celsius at 15 psi for 15-20 minutes
temperature should reach 100 degrees Celsius at 15 psi for 15-20 minutes
temperature should reach 200 degrees Celsius at 15 psi for 10 minutes
temperature should reach 100 degrees Celsius at 15 psi for 30 minutes
Jacob was asked to focus the specimen of Staphylocci under oil-immersion objective. He was unable to see the microorganisms clearly using the compound light microscope. What might be the problem in his technique?
The magnification he is using has x40 magnification
He placed 1 drop of immersion oil on the glass slide.
He used the fine adjustment knobs for focusing under OIO.
He adjusted the condenser focus knob to move the condenser up and down to control the focus of light on the specimen.
What is the function of the immersion oil in microscopy?
decrease the difference between the refractive indices of the glass slide and air
increase the difference between the refractive indices of the glass slide and air
allow scattering of light from the specimen
allow absorption of light emitted from the specimen
What is the total magnification if specimen is focused under high-power field if the magnifying power of its ocular lens is x10?
x 10,000
x 1,000
x 400
x 100
Spirochetes such as the causative agent of syphilis, Treponema pallidum, can be better seen under this type of microscope in which light reflected from the bacteria is seen against a dark background.
brightfield microscope
darkfield microscope
phase contrast microscope
fluorescence microscope
electron microscope
This type of microscope is mostly used in antigen-antibody reactions of microbes in clinical specimens.
brightfield microscope
darkfield microscope
phase-contrast microscope
fluorescence microscope
electron microscope
This is the ability of the lens system of a microscope to enable viewer to discriminate between two adjacent objects.
resolving power
magnification
absorbance
refractive power
Which of the following statement is NOT true regarding Koch’s postulate?
The isolated microorganism must cause the original disease when inoculated into a susceptible host.
The microorganism must always be found in similarly diseased animals but not in healthy ones.
The microorganism must be isolated from a diseased animal and grown in pure culture.
Life can arise spontaneously from nonliving material.
