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BSCi106 Quiz No. 3

Total questions: 22

Worksheet time: 26mins

Name
Class
Date
1.

Bacteria isolated from a hot tub at 39 °C are probably which of the following?

a)

thermophiles

b)

psychrotrophs

c)

mesophiles

d)

basophiles

e)

hyperthermophiles

2.

Which of the following is the reason jams and dried meats often do not require refrigeration to prevent spoilage?

a)

low pH

b)

toxic alkaline chemicals

c)

naturally occurring antibiotics

d)

low water activity

e)

high pH

3.

A soup container was forgotten in the refrigerator and shows contamination. The contaminants are probably which of the following?

a)

 thermophiles

b)

 acidophiles

c)

mesophiles

d)

psychrotrophs

e)

basophiles

4.

 In which environment are you most likely to encounter an acidophile?

a)

human blood at pH 7.2

b)

a hot vent at pH 1.5

c)

human intestine at pH 8.5

d)

milk at pH 6.5         

5.

Pseudomonas aeruginosa is a common pathogen that infects the airways of patients with cystic fibrosis. It does not grow in the absence of oxygen. The bacterium is probably which of the following?

a)

an aerotolerant anaerobe

b)

an obligate aerobe

c)

an obligate anaerobe

d)

a facultative anaerobe

6.

Which of the following is suitable for use on tissues for microbial control to prevent infection?

a)

disinfectant

b)

antiseptic

c)

sterilant

d)

 water

7.

Which of the following best describes a microbial control protocol that inhibits the growth of molds and yeast?

a)

bacteriostatic

b)

fungicidal

c)

bactericidal

d)

fungistatic

8.

Autoclaving pressure is usually set at

a)

15 psi at 121°C

b)

15 psi at 122°C

c)

15 psi at 123°C

d)

15 psi at 124°C

e)

15 psi at 125°C

9.

A microbicidal agent has what effect?

a)

sterilizes

b)

inhibits microorganisms

c)

is toxic to human cells

d)

destroys microorganisms

10.

Which of the following is the best definition of generation time in a bacterium?

a)

the length of time it takes to reach the log phase

b)

the length of time it takes for a population of cells to double

c)

the time it takes to reach stationary phase

d)

the length of time of the exponential phase

11.

EMB agar is a medium used in the identification and isolation of pathogenic bacteria. It contains digested meat proteins as a source of organic nutrients. Two indicator dyes, eosin and methylene blue, inhibit the growth of gram-positive bacteria and distinguish between lactose fermenting and nonlactose fermenting organisms. Lactose fermenters form metallic green or deep purple colonies, whereas the nonlactose fermenters form completely colorless colonies. EMB agar is an example of which of the following?

a)

a selective medium only

b)

a differential medium only

c)

a selective medium and a chemically defined medium

d)

a selective medium, a differential medium, and a complex medium

12.

A laboratory technician uses a MacConkey agar that is designed to suppress the growth of Gram-positive bacteria, and encourage the growth of Gram-negative microbes. MacConkey agar is a type of _____

a)

differential media

b)

nutrient agar

c)

 selective media

d)

nutritive media

e)

complex media

13.

Period of equilibrium in which new cell numbers are balanced by the death of cells is considered the ____

a)

growth phase.

b)

equilibrium phase

c)

log phase.

d)

stationary phase.

14.

Which growth phase includes an exponential growth due to the reproduction by binary fission (bacteria) or mitosis (yeast).

a)

lag phase

b)

stationary phase

c)

log phase

d)

prolonged death phase

15.

In binary fission, all of the following occur EXCEPT

a)

there is an increase in numbers.

b)

the bacteria cell invades a host cell.

c)

the resulting cells are genetically identical.

d)

only one parent cell was involved at the beginning.

16.

A plasmid that contains multiple genes for drug resistance is known as a

a)

Resistance factor

b)

Fertility factor

c)

Acquired resistance

d)

Intrinsic resistance

e)

Superbugs

17.

This refers to microbes (mainly bacteria) that have become resistant to one or more antimicrobial agents.

a)

Resistance factor

b)

Fertility factor

c)

Acquired resistance

d)

Intrinsic resistance

e)

Superbugs

18.

Ampicillin belongs to what type of antibiotics

a)

narrow spectrum

b)

broad spectrum

c)

multi-resistant

d)

all of the above

19.

This refers to the use of chemical agents to inhibit the growth of pathogens, either temporarily or permanently.

a)

chemical disinfection

b)

physical disinfection

c)

microbial disinfection

d)

none of the above

20.

This is used in hospitals and medical and dental clinics to clean equipment.

a)

Desiccation

b)

Radiation

c)

Ultrasonic waves

d)

Gaseous atmosphere

21.

This results in dried microorganisms that remain viable, but cannot reproduce

a)

Desiccation

b)

Radiation

c)

Ultrasonic waves

d)

Gaseous atmosphere

22.

Discuss different reasons why antimicrobial agents should not be used indiscriminately. (5pts.)

4 lines