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Basic Immunologic response

Total questions: 24

Worksheet time: 12mins

Name
Class
Date
1.

* use of labeled monoclonal antibodies against specific surface antigens

* light scattering is measured as cells flow through a laser beam

a)

Cell Flow Cytometry

b)

Rosette Technique

c)

Direct or Indirect Immunofluorescence

d)

Surface Immunoglobulins

e)

Density gradient centrifugation with Ficoll-Hypaque

2.

* old method

* T lymphocytes form rosettes with sheep's RBC (CD2- receptor for sheep's RBC)

a)

Cell Flow Cytometry

b)

Rosette Technique

c)

Direct or Indirect Immunofluorescence

d)

Surface Immunoglobulins

e)

Density gradient centrifugation with Ficoll-Hypaque

3.

* use of labeled monoclonal antibodies against specific surface antigens

* slides are read with a fluorescent microscope

a)

Cell Flow Cytometry

b)

Rosette Technique

c)

Direct or Indirect Immunofluorescence

d)

Surface Immunoglobulins

e)

Density gradient centrifugation with Ficoll-Hypaque

4.

* immunoenzymatic method

* demonstrate immunoglobulins on B cells (Y-shaped molecule)

a)

Cell Flow Cytometry

b)

Rosette Technique

c)

Direct or Indirect Immunofluorescence

d)

Surface Immunoglobulins

e)

Density gradient centrifugation with Ficoll-Hypaque

5.

* to separate B to T

* use nylon wool as a strainer to filter the lymphocytes

a)

Cell Flow Cytometry

b)

Rosette Technique

c)

Direct or Indirect Immunofluorescence

d)

Surface Immunoglobulins

e)

Density gradient centrifugation with Ficoll-Hypaque

6.

*monocytes, macrophages, DCs

* inflammation

* fever initiation of the acute phase response

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

7.

monocytes

macrophages

neutrophils

NK cells

activated T cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

8.

monocytes macrophages

endothelial cells

Th2 cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

9.

T cells

macrophages

other cell types

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

10.

macrophages

DCs

virally infected cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

11.

*inflammation

* initiation of the acute phase response

* death of tumor cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

12.

* initiation of acute phase response

* activation of B and T cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

13.

* inihibition of both T and B cells proliferation

* introduction of IgA

* inhibition of macrophages

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

14.

* protects cells against viruses

* increases class I MHC expression

* activates NK cells

a)

Interleukin-1β

b)

Tumor necrosis factor - alpha

c)

Interleukin - 6

d)

Transforming growth factor-beta

e)

Interferon - alpha

Interferon - beta

15.

Secreted by T cells

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

16.

* growth and proliferation of T and B cells

* NK activation and proliferation

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

17.

*Th2 cells

* mast cells

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

18.

* promotion of Th2 differantiation

* stimulation of B cells to which to IgE production

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

19.

Th2 cells

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

20.

* eosinophil generation and activation

*B- cell differentiation

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

21.

* Th2 cells

* monocytes

* macrophages

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

22.

* suppression of Th2 cells

* inhibition of antigen presentation

* inhibition of IFN-γ

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

23.

* Th1 cells

* CD8+ T cells

*NK cells

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

24.

* activation of macrophages

* increased expression of Class I and II MHC molecules

* increased antigen presentation

a)

Interleukin - 2

b)

Interleukin - 4

c)

Interleukin - 5

d)

Interleukin - 10

e)

Interferon - gamma

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