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WorksheetsTransformation - iGEM Thessaloniki
Total questions: 10
Worksheet time: 7mins
After the heat shock we incubate the cells on ice to recover for:
2 minutes
30 minutes
45 seconds
1 hour
We add CaCl2 because Cl increases the permeability of the membrane
True
False
The temperature of the water bath for the heat shock has to be:
(E.Coli)
30°C
50°C
42°C
Transformation is applicable to eukaryotic cells
True
False
To endure the heat shock treatment the cells used are in:
The log phase of growth
The lag phase of growth
The stationary phase of growth
We use IPTG (isopropyl - β- D-1- thiogalactopyranoside) to :
makes the membranes permeable
trigger the transcription of the lac operon
recover the cells after the heat shock
After we apply high- voltage electric pulse to the cells we incubate them in ice to recover :
True
False
In blue-white screening, the blue colonies are bacterias
with recombinant plasmids
with non-recombinant plasmids
with no plasmids
Which methods can be used to detect recombinant vectors?
Western blot
Restriction enzymes + electrophoresis
Plasmid with lethal gene
Electroporation
Sanger sequencing is the most accurate method to detect recombinant vectors
True
False
