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BIO 30_Lec 5_DNA Importance, Events, and Scientists

Total questions: 27

Worksheet time: 20mins

Name
Class
Date
1.

In 1830s, it was regarded as the most important molecules.

(a)  

2.

Greek for "of first importance"

(a)  

3.

Scientist that stated 6 characteristics of genetic material (slide)

(a)  

4.

1860s, while characterizing proteins from pus cells, a molecule different from protein was isolated from the nucleus and called it "nuclein" (DNA)

a)

Fredrich Miescher

b)

Earnst Haeckel

c)

Edmund Wilson

d)

Fredrerick Griffith

5.
1866, discovered that the most obvious cellular component of a cell was nucleus
a)

Earnst Haeckel

b)

Fredrich Miescher

c)

Edmund Wilson

d)

Fredrerick Griffith

6.

1895, using staining technique, he discovered that the most important nuclear element handed from cell to cell was the DNA. Observed through the karyokinesis of ovum.

a)

Edmund Wilson

b)

Fredrich Miescher

c)

Earnst Haeckel

d)

Fredrerick Griffith

7.

1928, US Medical Officer did transformation experiment (on mouse) using Streptococcus pneumoniae and declared the presence of *transforming principle*. (Rough->Smooth)

a)

Fredrerick Griffith

b)

Fredrich Miescher

c)

Earnst Haeckel

d)

Edmund Wilson

8.
1928 Experiment where heat killed virulent S + regular avirulent R cells into mice = death of mice
a)
Transformation Experiment
b)
Blender Experiment
c)
Transduction Experiment
9.

1930s, _._. (a)   , a quantitative geneticist stated two points about genes:

1. Hypothetical Entity

2. Chemical compound

10.
1944, Transforming Principle (TP) was DNA. DNA treatment under protein and RNA enzyme = ability retained. DNA treated under DNA enzyme = ability lost.
a)
Oswald Avery, Colin Macleod, and Maclyn McCarthy
b)
Alfred Hershey & Martha Chase
c)
Norton Zinder, Joshua and Esther Lederberg
d)
Rosalind Franklin, Maurice Wilkins, James Watson, and Francis Crick
11.

1952, Blender experiment, they proved that the DNA is the genetic material of bacterial viruses which is injected into the cell during infection. Labelled DNA with P32 and protein coat with 35S.

a)

Oswald Avery, Colin Macleod, and Maclyn McCarthy

b)

Alfred Hershey & Martha Chase

c)

Norton Zinder, Joshua and Esther Lederberg

d)

Rosalind Franklin, Maurice Wilkins, James Watson, and Francis Crick

12.
1952 Experiment where radiation imaging revelead that protein coat remained outside while the DNA was injected to the cell
a)
Blender Experiment
b)
Transformation Experiment
c)
Transduction Experiment
13.
1952, using transduction experiment in Salmonella typhimurium further proved that DNA is the Genetic material.
a)
Oswald Avery, Colin Macleod, and Maclyn McCarthy
b)
Alfred Hershey & Martha Chase
c)
Norton Zinder, Joshua and Esther Lederberg
d)
Rosalind Franklin, Maurice Wilkins, James Watson, and Francis Crick
14.
1952 Experiment where bacteriophage infects a bacterial cell, a transducing phage picks a gene, infects and integrates the gene into a recepient cell.
a)
Transduction Experiment
b)
Blender Experiment
c)
Transformation Experiment
15.
1953 Structure of DNA was discovered
a)
Oswald Avery, Colin Macleod, and Maclyn McCarthy
b)
Alfred Hershey & Martha Chase
c)
Norton Zinder, Joshua and Esther Lederberg
d)
Rosalind Franklin, Maurice Wilkins, James Watson, and Francis Crick
16.
Ph. D. in Biophysics and started to work on x-ray studies of proteins at Cambridge Univ. in 1947
a)
Francis Crick
b)
James D. Watson
c)
Maurice Wilkins
d)
Rosalind Franklin
17.
PhD. in Microbiology from Univ. of Indiana and in 1951 moved to Cambridge Univ. and shared office with Crick.
a)
Francis Crick
b)
James D. Watson
c)
Maurice Wilkins
d)
Rosalind Franklin
18.
Ph. D. in Physics worked on radar and Manhattan Project and assistant director of Medical Research at Kings College, London. Worked on X-Ray diffraction of DNA.
a)
Francis Crick
b)
James D. Watson
c)
Maurice Wilkins
d)
Rosalind Franklin
19.
Expert on X-Ray diffraction techniques and joined Kings College in 1951 to work on x-ray diffraction of DNA
a)
Francis Crick
b)
James D. Watson
c)
Maurice Wilkins
d)
Rosalind Franklin
20.
Helical DNA structure, sugar, and Phosphate background outside, while nucleotides are tucked inside.
a)
Rosalind Franklin, Gosling, and Maurice Wilkins
b)
James watson and Francis Crick
c)
Linus Pauling
21.
Triple helix, Phosphate in center.
a)
Rosalind Franklin, Gosling, and Maurice Wilkins
b)
James watson and Francis Crick
c)
Linus Pauling
22.
Triple helix.
a)
Rosalind Franklin, Gosling, and Maurice Wilkins
b)
James watson and Francis Crick
c)
Linus Pauling
23.
Nucleotides are flat, stack up on top of another, possibility of A=T, C=-G pairing
a)
John Griffith
b)
Erwin Chargaff
c)
Jerry Donohue
24.
Purine-Pyrimidine ratio is 1:1
a)
John Griffith
b)
Erwin Chargaff
c)
Jerry Donohue
25.
H bases can change positions = H bonding
a)
John Griffith
b)
Erwin Chargaff
c)
Jerry Donohue
26.

In 1957, they reconstructed the TMV A and B virus and found that:

1. RNA A with B coat = Type A progeny

2. RNA B with A coat = Type B progeny

a)

Heinz Frankel-Conrat and B. Singer

b)

Alfred Hershey and Martha Chase

c)

Oswald Avery, Colin MacLeod

and Maclyn McCarty

d)

Norton Zinder, Joshua and

Esther Lederberg

27.

In 1958, they proved that the DNA was replicated in a semi-conservative manner by growing E. coli in 15N and 14N. They isolated the DNA and centrifuged it in cesium chloride. The different densities per generation showed heavy, hybrid, and hybrid markers.

a)

Norton Zinder, Joshua and

Esther Lederberg

b)

Matthew Meselson and

Franklin Stahl

c)

Erwin Chargaff and Jerry Donohue

d)

Linus Pauling and Francis Crick