WorksheetsHISTOPATH- MOUNTING
Total questions: 16
Worksheet time: 8mins
Name
Class
Date
1.
A syrupy fluid applied between the section and the coverslip after staining, setting the section firmly, preventing the movement of the coverslip. Necessary to protect the stained section from physical injury and from bleaching or deterioration of the stain as a result of oxidation
a)
Mounting
b)
Mountant
2.
For best results with stained tissue sections, they must be impregnanted by a transparent medium having an RI (Refractive Index) close to that of glass.
a)
1.518
b)
1.298
3.
Designed to mount water-miscible preparations directly from water in cases where the stain is removed or decolorized with alcohol or xylene.
a)
Aqueous/Water Soluble Mountants
b)
Resinous Mountants
4.
Used for preparations that have been dehydrated and cleared in xylene or toluene and are recommended for majority of staining methods.
a)
Aqueous/Water Soluble Mountants
b)
Resinous Mountants
5.
Low refractive index; good only for temporary mounting
a)
Water
b)
Glycerin
c)
Farrant’s Medium
d)
Apathy’s Medium
e)
Brun’s Fluid
6.
(1.46 RI) Also used as preservative, has high index of refraction; used when dehydration and clearing with xylene cannot be made
a)
Water
b)
Glycerin
c)
Farrant’s Medium
d)
Apathy’s Medium
e)
Brun’s Fluid
7.
(Gum syrup) (RI:1.43) Does not solidify upon storage
a)
Water
b)
Glycerin
c)
Farrant’s Medium
d)
Apathy’s Medium
e)
Brun’s Fluid
8.
(1.53 RI) Used for Methylene Blue-stained nerve preparations and as general purpose aqueous mountant
a)
Water
b)
Glycerin
c)
Farrant’s Medium
d)
Apathy’s Medium
e)
Brun’s Fluid
9.
Recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing
a)
Water
b)
Glycerin
c)
Farrant’s Medium
d)
Apathy’s Medium
e)
Brun’s Fluid
10.
(RI: 1.524) Natural resin extracted from the Canadian Tree, Abus balsamea - Recommended for whole mounts and for thick sections because it does not shrink much - Miscible with xylene and is quite expensive
a)
Canada Balsam
b)
DPX
c)
XAM
d)
Clarite
11.
(RI:1.532) Recommended for small tissue sections
a)
Canada Balsam
b)
DPX
c)
XAM
d)
Clarite
12.
(RI:1.52) Synthetic resin mixture in xylene in pale yellow or colorless solution
a)
Canada Balsam
b)
DPX
c)
XAM
d)
Clarite
13.
(RI:1.544) Synthetic resin which is soluble in xylene (usually diluted to 60% with xylene)
a)
Canada Balsam
b)
DPX
c)
XAM
d)
Clarite
14.
Mounting media most commonly used today:
a)
Eukitt
b)
Entellan
15.
Process of sealing the margins of the coverslip to prevent escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip, and to prevent sticking of slides upon storage.
a)
RINGING
b)
SEALING
16.
Made up of 2 parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered
a)
Kronig Cement
b)
Asphalt Cement
100 %
