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Mycology Lab Quiz

Total questions: 20

Worksheet time: 5mins

Name
Class
Date
1.

Mold occur in long filaments known as hyphae, which grow by apical extention.

True or False?

a)
True
b)
False
2.

Negative Interpretation for urease test

a)

b)

c)

d)

3.

Suitable temperature for fungal incubation

a)

32°C

b)

35°C

c)

37°C

d)

39°C

4.

What are the biochemical tests for yeast identification?

a)

Urease Test & LPCB

b)

Germ Tube Test & Indian Ink

c)

Germ Tube Test & Urease Test

d)

Indian Ink & LPCB

5.

Fungi reproduce using

a)

Seeds

b)

Spores

c)

Eggs

6.

Which fungus is urease positive?

a)

Candida spp.

b)

Escherichia Coli

(E.coli)

c)

Yersinia Pestis

d)

Cryptococcus Neoformans

7.

The study of Fungi is called

a)

Hyphology

b)

Fungology

c)

Mycology

d)

Mushology

8.

Which statement below is true about germ tube test?

a)

A germ tube test is a diagnostic test in which a sample of fungal spores are suspended in animal serum

b)

Germ tube has a constriction

c)

No hyphal (filamentous) extention arising from a yeast cell

9.

Which of the following is the true order of Gram Stain?

a)

b)

c)

d)

10.

How long should we spin the CSF sample before applying Indian Ink for microscopic identification?

a)

3 - 5 minutes

b)

5 - 8 minutes

c)

3 - 7 minutes

d)

5 - 10 minutes

11.

Most fungi are multicellular organisms.

This means they are made of:

a)

One cell

b)

No cells

c)

More than one cell

12.

What are two method used in Lactophenol Cotton Blue (LPCB) staining?

a)

Tease mount &

KOH 20% wet mount

b)

Tease mount &

scotch tape

c)

KOH 20% wet mount &

scoth tape

d)

VITEK MS & tease mount

13.

VITEK MS is applied if all the microscopic identification is unsuccessful.

True or false?

a)

False

b)

True

14.

Which statement below is incorrect?

a)

SDA:

Primary culture medium for fungi

b)

Urease slant:

to differentiate urease negative candida & urease positive cryptococcus

c)

Corn Meal Agar:

medium used for slide culture to encourage mold reproduce asexually

15.

This colony characteristic is usually produced by _________

a)

Virus

b)

Bacteria

c)

Yeast

d)

Mold

16.

Identify

a)

Cryptococcus neoformans

b)

Candida spp

c)

Trichosporon

d)

Rhodotorula

17.

When using the Germ Tube method, this yeast species can be seen.

Identify

a)

Candida tropicalis

b)

Candida glabrata

c)

Candida albicans

18.

Which staining technique should be used to identify yeast?

a)

KOH 20% Wet Mount

b)

Lectophenol Cotton Blue Staining

c)

Tease Mount

d)

Indian Ink

19.

This method is used for Antifungal Susceptibility Testing.

What does it mean if the reaction turns to blue?

a)

No Growth

b)

Partial inhibition

c)

Growth

20.

Based on the graphic below, yeast can be seen using…

a)

Indian Ink Stain

b)

Lectophenol Cotton Blue Staining

c)

Gram Stain

d)

Giemsa Stain