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Basic Molecular Diagnostics

Total questions: 10

Worksheet time: 2mins

Name
Class
Date
1.

A process wherein DNA is "converted" to RNA.

a)

Replication

b)

Transcription

c)

Conversion

d)

Translation

2.

TRUE OR FALSE

  1. 1. Proteins are composed of amino acids which are attached to one another in long chains.

  2. 2. A sequence of three nucleotides which form a unit of genetic code in the DNA/RNA is called codons.

a)

Statement 1 is true. Statement 2 is false.

b)

Statement 1 is false. Statement 2 is true.

c)

Both statements 1 and 2 are true.

d)

Both statements 1 and 2 are false.

3.

All of the following DNA base pairings are correct, EXCEPT:

a)

Adenine - Thymine

b)

Cytosine - Guanine

c)

Adenine - Uracil

d)

Thymine - Guanine

4.

Which of the following nucleotide is/are seen in RNA?

a)

Adenine

b)

Thymine

c)

Uracil

d)

Cytosine

5.

What type of mutation?

ACAAGC - ACAGC

a)

SNP

b)

Substitution

c)

Insertion

d)

Deletion

6.

What type of mutation?

GCGGAC - GGCGGAC

a)

SNP

b)

Substitution

c)

Insertion

d)

Deletion

7.

Which of the following is a start codon?

a)

UAA

b)

UAG

c)

AUG

d)

AUA

8.

Which of the following PCR is being employed in TrueNat technology?

a)

Conventional PCR

b)

Real-time PCR

c)

Reverse-transcription PCR

d)

Nested PCR

9.

This is an enzyme that elongates primers based on the DNA template.

a)

Polymerase

b)

Ligase

c)

Nuclease

d)

None of the above.

10.

Arrange the following steps of PCR processing correctly.

  1. 1. Annealing

  2. 2. Denaturation

  3. 3. Elongation

a)

123

b)

213

c)

231

d)

321