WorksheetsProtein Purification (Biotechnolagy)
Total questions: 10
Worksheet time: 6mins
What do Transgenic Cells make?
Cells
Membranes
Foreign Proteins
Reaction Enzymes
What is Centrifugation?
technique used to separate
molecules on the basis of size on density by
high speed spinning (applying centripetal
acceleration force)
What is it called when the denser or heavier particles will go
the bottom?
Pallet
What is TWO safe use of microcentrifuges in a lab?
LOAD THE ROTOR CORRECTLY
– balance with the tubes with same
amounts of water if needed
If it vibrates or jumps – keep it on
Make sure the centrifuge is set on
leveled steady surface
Open the lid while the
centrifuge is spinning
What is Chemical Breakdown
lysozyme or similar
enzymes (enzymes that break down cell walls
and membranes)
homogenization
What is Filtration?
passing of particles
through materials with passages of
certain sizes using gravity force or aiding
with vacuum suction force
What is Centrifugation?
debris in a
cell
debris in a
pellet
debris in a
enzyme
debris in a
membrane
What are some buffers used in HIC
Equilibration buffer,Binding buffer,Wash buffer,Elution buffer
What is some examples of Protein separation using chromatography?
Size exclusion
Ion stealing
Hydrophobic interaction
Affinity
chromatography
What does HIC mean in Biotech?
Hydrophobic interaction chromatography
