wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

ECH4201 Topic 7 (Purification Technique- Chromatography)

Total questions: 50

Worksheet time: 32mins

Name
Class
Date
1.

Chromatography can be used for separation in lab and industry

a)

True

b)

False

2.

Which one is stationary phase?

a)

Methanol

b)

Silica gel

c)

Hexane

3.

Classification of chromatography based of mobile phase

a)

Liquid chromatography

b)

Gas chromatography

c)

Solid Chromatography

4.

The following is something we need to consider for choosing mobile phase except

a)

Polarity interaction

b)

Eluotropic series

c)

Polarity prediction of mixed solvent

d)

Stationary phase

5.

Which one is industry commonly using chromatography?

a)

Pharmaceutical

b)

Food

c)

Construction

6.

Retention factor can be used for

a)

Indentifcation unknown compound

b)

Polarity compound

c)

None of above

7.
The mobile phase in paper chromatography is typically a
a)
plasma
b)
solid
c)
gas
d)
liquid
8.
The substance in the mixture that has the greatest affinity for the mobile phase will travel
a)
fastest
b)
slowest
9.

Ion-Exchange chromatography:

a)

separate and analyze compounds that can be vaporized without decomposition

b)

Also known as Liquid-Solid Chromatography

c)

Materials used are either cation or anion resins

d)

Separation based on partition coefficient between 2 immiscible liquids

10.

What type of chromatography is depicted by the illustration?

a)

Gel Filtration Chromatography

b)

Column Chromatography

c)

HPLC

d)

Thin Layer Chromatography

11.

Which of the following methods could be used to check the molecular weight of your purified protein?

a)

SDS-PAGE

b)

Mass Spectrometry

c)

Analytical SEC

d)

All of above

12.

To elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

a)

Low salt concentrations

b)

High salt concentrations

c)

Adding a soluble ligand which competes with the affinity tagged protein for binding to the column

d)

Just keep washing buffer through the column, isocratic elution

13.

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

a)

Prediction of isoelectric point (pI) from the amino acid sequence

b)

Test protein binding to an IEX matrix at a range of pHs and salt concentrations

c)

Test protein binding to a selection of anion and cation exchange matrices

d)

Pass your sample through a preparative column and elute with a salt gradient

14.

You find that your protein sample loses activity during storage. What can you do about this?

a)

Add an additional purification step

b)

Use a protease inhibitor during purification steps

c)

Perform each step as quickly as possible, in a cold-room

d)

All of the above

15.

Hydrophobic amino acids are ones which _______ water.

a)

are attracted to

b)

are repelled by

c)

react with

d)

melt in

16.
A low retention time indicates
a)
a high affinity to the mobile phase
b)
a low affinity to the stationary phase
c)
a high solubility in the mobile phase
d)
all of the above
17.

What is chromatography?

a)

A way to see pretty colors

b)

A laboratory technique used to separate a mixture.

c)

A form of distillation

18.
Why is Chromatography useful?
a)
It can be used as medicine.
b)
It can be used for finding clues at a crime scene
c)
It can be used to make bombs.
d)
It can reduce the lack of food and water.
19.
Whose blood was found at the crime scene?
a)
John
b)
Lisa
c)
Bob
d)
Sure
20.
Separates gaseous substances in a calibrated column into different components
a)
Thin Layer Chromatography
b)
HPLC
c)
Gas Chromatography
d)
Mass Specroscopy
21.
Which forms of Chromatography give qualitative and quantitative results?
I paper chromatoraphy
II TLC
III HPLC
IV GC
a)
I and II
b)
III and IV
c)
II only
d)
III only
22.

Which technique is commonly used to analyze metal ions and organic compounds in solutions?

a)

Liquid Chromatography

b)

Gas Chromatography

c)

Thin-layer Chromatography

d)

Paper Chromatography

23.

Using a salt gradient is most commonly used to elute proteins in which chromatography method?

a)

HPLC

b)

affinity

c)

Ion Exchange

d)

size exclusion

24.

Which chromatography method relies on specific binding between a protein and a complimentary substrate/ligand?

a)

HPLC

b)

affinity

c)

ion exchange

d)

size exclusion

25.

Which method would pass a liquid solvent through the stationary phase at high pressure?

a)

HPLC

b)

affinity

c)

ion exchange

d)

size exclusion

26.

In size exclusion chromatography, why do large proteins elute first?

a)

they travel through the void of stationary phase matrix so pass through the column quicker

b)

they enter the porous matrix of the stationary phase so pass thorugh column quicker

c)

small particles bind to the porous matrix of the stationary phase meaning large will elute first

d)

they don't elute firts, the smaller proteins do.

27.

A cation exchange column has which kind of charge?

a)

postive

b)

negative

28.

An anion exchange column carries what kind of charge?

a)

positive

b)

negative

29.

In cationic exchange which ion from the salt wash would outcompete the proteins bound to the column, causing them to elute

a)

Na+

b)

Cl-

30.

Which polymer is often used for matrix materials in Affinity chromatography

a)

Agarose

b)

cellulose

c)

dextrose

d)

all of the above

31.

To elute target proteins from an affinity chromatography matrix, which of the following elution methods is LEAST appropriate?

a)

changing pH

b)

Increasing ionic strength (e.g increasing NaCl gradient)

c)

Using a competitive ligand (e.g competitive inhibitor)

d)

isocratic elution (continual application of wash buffer)

32.

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?

a)

Affinity chromatography (AC)

b)

Ion-exchange chromatography (IEX)

c)

Hydrophobic interaction chromatography (HIC)

d)

Size-exclusion chromatography (SEC)

33.

What are the two(2) important phases of chromatography ?

a)

mobile and inert phase

b)

flexible and immobile phase

c)

mobile and stationary phase

d)

stationary and indestructible phase

34.
The retention factor is used
a)
to zero the chromatography scale
b)
to identify unknown compounds in a sample
c)
to retain solvent
d)
as a starting tool for chromatography
35.
If a dye is insoluble in water and cannot be separated using chromatography, how can it be tested to see if it is a mixture?
a)
Use filtration instead
b)
Repeat the chromatography test using a different solvent such as propanone
c)
Use distillation instead
d)
Repeat the chromatography using water as the solvent and see if it separates this time
36.
In chromatography, if a solute does not separate and remains on the start line ....
a)
the solute is insoluble in the solvent
b)
the solute is soluble in the solvent
c)
the solvent is insoluble in the solute
d)
the solvent is soluble in the solute
37.
A qualitative test will reveal....
a)
identity of a material
b)
amount of a sample
c)
usefulness of a compound
d)
purity of a substance
38.
Which of the following can be used to find the concentration of a substance in HPLC
a)
Rf value
b)
Rt value
c)
peak area
d)
peak width
39.
In order to compare to chromatograms what must be kept the same?
a)
stationary  phase
b)
mobile phase
c)
temperature
d)
all of the above
40.

Fruit drink colored orange contains a dissolved of red and yellow coloring coloring agents. One of these coloring agents is suspect of being illegal. Which method could be used to show the presence of this illegal coloring agent?

a)

Filtration

b)

Simple distillation

c)

Evaporation

d)

Chromatography

41.

Ion-Exchange chromatography:

a)

separate and analyze compounds that can be vaporized without decomposition

b)

Also known as Liquid-Solid Chromatography

c)

Materials used are either cation or anion resins

d)

Separation based on partition coefficient between 2 immiscible liquids

42.

Stationary Phase

a)

System modules

b)

Column

c)

Solvents

d)

Detector

43.

Resolution

a)

Capacity to separate an certain quantity of analyte

b)

Selectivity of stationary phase towards a specific analyte

c)

Efficiency of chromatography to separate compounds as quick as possible

d)

Ability to identify individual analyte

44.

Chromatogram

a)

A picture of lights

b)

Method development

c)

A set of properties for a run

d)

A pattern of separation

45.

Baseline

a)

Starting spectra of an analysis

b)

Starting datapoints of an analysis

c)

The standard timepoint of an analysis

d)

The standard (sample) of an analysis

46.

Void volume

a)

Empty space in the galaxy

b)

Empty space in the column

c)

Empty space in the solvents

d)

Empty space throughout the chromatography

47.

Elution

a)

Analyte injection time

b)

Analyte retention time

c)

Analyte exit time

d)

Analyte preparation time

48.

Retention time

a)

Time spent between injection and column

b)

Time spent inside column only

c)

Time spent between column and detector

d)

Time spent from injection until detection

49.

 Retardation value (Rf) is determined by

R=XYR=\frac{X}{Y}

 

                                  

a)

x = weight of molecule;

y= size of molecule

b)

x= length of molecule;

y=weight of sample

c)

x= distance travelled by substance;

y= distance travelled by solvent

d)

x= concentration of sample;

y= concentration of solvent

50.

What is Eluent?

a)

Is a Liquid solution

b)

Is a Liquid Solution that is a result from Elution

c)

It is a solvent used for separation of absorbed material from stationary phase

d)

None of the above

Similar Resources on Wayground

MAD DOGS AND ENGLISHMEN ROUND 1

50 questions

MAD DOGS AND ENGLISHMEN ROUND 1

quiz

Worksheet

•

Professional Development

Soal Soal PPPK 2021 (2)

45 questions

Soal Soal PPPK 2021 (2)

quiz

Worksheet

•

University - Professi...

SKN MANAGER

50 questions

SKN MANAGER

quiz

Worksheet

•

Professional Development

Chapter 44 practice quiz Part 2

50 questions

Chapter 44 practice quiz Part 2

quiz

Worksheet

•

Professional Development

PMLE 1-50

50 questions

PMLE 1-50

quiz

Worksheet

•

Professional Development

Marvel

46 questions

Marvel

quiz

Worksheet

•

KG - Professional Dev...

Selection

50 questions

Selection

quiz

Worksheet

•

Professional Development