WorksheetsMI 3.1.4 & 3.1.5 Microarrays
Total questions: 19
Worksheet time: 14mins
What is transcribed from DNA to produce a protein and is a good indication of whether or not a particular gene is expressed?
mRNA
cDNA
tRNA
trRNA
What happens after the DNA from the sample is applied to the DNA microarray?
the mRNA falls off the spot
the DNA comes apart
the tRNA will join the complementary strand
the cDNA will hybridize with the single stranded DNA molecules on the microarray
In the microarray, what does the RED color indicate?
these genes are not expressed or turned off
these genes are expressed or turned on in cancer
these genes are expressed in healthy cells
there are no genes in this cell
What does the GREEN color indicate in DNA microarray?
these genes are expressed in cancer
these genes are not expressed in cancer
these genes are not expressed in healthy cells
there are no genes in these cells
What does the YELLOW color indicate in DNA microarray?
this gene is expressed in cancer cells only
this gene is expressed in healthy cells only
there are no genes in the cell
this gene is expressed in both cancer and healthy cells
What does BLACK indicate in dna microarray?
gene expression in cancer cells
gene expression in healthy and cancer cells
no gene expression
gene expression in healthy cells
Which genes are expressed in both normal and in cancerous cells?
Genes marked by red spots only
Genes marked by red and green spots
Genes marked by black and yellow spots
Genes marked by yellow spots only
Which unknown gene sequences might belong to cancer-suppressor genes?
Genes marked by red spots
Genes marked by green spots
Genes marked by black spots
Genes marked by yellow spots
When comparing healthy and cancerous lung cells on a microarray, what color would you expect the dot that represents the gene for producing melanin (pigment for skin color)?
Red
Yellow
Green
Black
DNA Microarray is also called as
DNA Chip
RNA Chip
Protein Chip
None
Reorder the following steps of a microarray experiment
Collect tissue (normal & cancerous)
Isolate RNA and separate tRNA, mRNA, and r RNA
Use reverse transcriptase to make a labeled DNA copy (cDNA)
Apply cDNA to the chip
Scan microarray and analyze DATA
When quantifying the microarray results, scientists convert the colors to what?
Match the following microarray data with the correct intensities
400
very bright
100
dull
250
medium
Match the ratio expression levels with the proper descriptions
Up regulation
positive ratio
Down regulation
negative ratio
gene is not affected by the tumor
ratio = 1
gene is not expressed in either cell
ratio = 0
This is the measure of degree of the relationship between two variables.
a. Johnson’s correlation coefficient
b. Pearson’s correlation coefficient
c. standard correlation coefficient
d. Spearman Rho coefficient
What is the strength and direction of correlation between two variables having a correlation coefficient value of 0.67?
a. perfect negative correlation
b. weak positive correlation
c. very weak positive correlation
d. strong positive correlation
Which two genes shown have the highest similarity after calculating correlation coefficient data?
C and A
B and D
D and A
B and C
What is the name of the type of diagram shown?
(a)
Match the following correlation coefficient values with the correct description
Correlation coefficient is positive
the profiles behave similarly
Correlation coefficient is negative
the profiles behave in opposite ways
Correlation coefficient is 1
the profiles are identical
Correlation coefficient is 0
profiles behave in an unrelated manner
