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Topic 2: Amino acids and Peptides

Total questions: 13

Worksheet time: 8mins

Name
Class
Date
1.

Which of the following amino acids has a side chain with a pKa around 4.1?

a)

Lysine

b)

Aspartic acid

c)

Histidine

d)

Arginine

2.

What is the isoelectric point for the amino acid Tryptophan?

pKa1 = 2.38

pKa2 = 9.39

a)

2.38

b)

7.07

c)

5.89

d)

9.39

3.

Which amino acid has a molecular weight of approximately 89 Da?

a)

Glycine

b)

Alanine

c)

Serine

d)

Valine

4.

What is the correct ionization state of histidine at pH = 1?

a)

-1

b)

0

c)

+1

d)

+2

5.

Which amino acid is most likely to be found in the interior of a protein due to its hydrophobic nature?

a)

Glutamine

b)

Leucine

c)

Serine

d)

Lysine

6.

What is the reaction that describes the formation of a peptide bond?

a)

Hydrolysis

b)

Condensation

c)

Oxidation

d)

Reduction

7.

Which amino acid has a side chain that can form disulfide bonds?

a)

Methionine

b)

Cysteine

c)

Proline

d)

Histidine

8.

Which of the following amino acids has a basic side chain?

a)

Glutamic acid

b)

Arginine

c)

Asparagine

d)

Phenylalanine

9.

This is the structure of GABA.

Is GABA an amino acid?

a)

Yes

b)

No

c)

Maybe

d)

I’m not sure

10.

Which of the following is a characteristic feature of the amino acid proline that distinguishes it from other standard amino acids?

a)

It contains a sulphur atom in its side chain.

b)

It has a secondary amine group.

c)

It is the only amino acid with a hydroxyl group in its side chain.

d)

It has an aromatic ring in its side chain.

11.

Which of the following conditions would most likely destabilise a disulfide bond in a protein?

a)

High pH

b)

Reducing environment

c)

High temperature

d)

Oxidising environment

12.

The Henderson-Hasselbalch equation can be used to determine the pH of a buffer solution. If the concentration of the conjugate base is twice that of the weak acid, what is the pH relative to the pKa?

a)

pH=pKa1pH = pK_a - 1

b)

pH=pKa+log10 0.5pH=pK_a+\log_{10}\ 0.5

c)

pH=pKapH = pK_a

d)

pH=pKa+log10 2pH=pK_a+\log_{10}\ 2

13.

In electrophoresis, which factor most determines the migration rate of an amino acid in a gel?

a)

The molecular weight of the amino acid

b)

The net charge of the amino acid

c)

The hydrophobicity of the amino acid

d)

The presence of disulfide bonds in the amino acid