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Hematochrit and Thin Blood Smear

Total questions: 21

Worksheet time: 11mins

Name
Class
Date
1.

What is the purpose of the 'Buffy Coat Method'?

a)

To identify bacteria in water samples

b)

To measure blood glucose levels

c)

To identify microfilaria and blood protozoa (Trypanosoma spp.)

d)

To determine blood pressure

2.

At what speed should the centrifuge be set during the 'Buffy Coat Method'?

a)

5,000-7,000 rpm

b)

10,000-12,000 rpm

c)

15,000-17,000 rpm

d)

20,000-22,000 rpm

3.

What is used to seal one end of the capillary tube in the 'Buffy Coat Method'?

a)

Glue

b)

Tape

c)

Sealant

d)

Wax

4.

What magnifications are used to examine the buffy coat under a microscope?

a)

x50 and x100

b)

x100 and x200

c)

x200 and x400

d)

x400 and x800

5.

What is the purpose of creating a thin blood smear?

a)

To identify blood protozoa and microfilaria

b)

To measure blood pressure

c)

To test blood sugar levels

d)

To determine blood type

6.

What is the first step in preparing a thin blood smear?

a)

Dry the slide

b)

Place a drop of blood at the edge of the glass slide

c)

Stain the slide with Giemsa

d)

Examine the slide under a microscope

7.

At what angle should the spreader be placed on the blood drop during the preparation of a thin blood smear?

a)

30 degrees

b)

45 degrees

c)

60 degrees

d)

90 degrees

8.

What magnification is used to examine the slide under a compound microscope?

a)

x400

b)

x600

c)

x800

d)

x1000

9.

Which of the following species is shown in the first image of the thin blood smear?

a)

Trypanosoma sp.

b)

Theileria sp.

c)

Babesia sp.

d)

Plasmodium sp.

10.

What is the species shown in the second image of the thin blood smear?

a)

Babesia sp.

b)

Theileria sp.

c)

Trypanosoma sp.

d)

Leishmania sp.

11.

Identify the species shown in the third image of the thin blood smear.

a)

Theileria sp.

b)

Babesia sp.

c)

Trypanosoma sp.

d)

Plasmodium sp.

12.

What is the purpose of a 'Thick Blood Smear'?

a)

To identify blood glucose levels

b)

To detect protozoa in blood

c)

To measure blood pressure

d)

To determine blood type

13.

Which stain is used in the 'Thick Blood Smear' process?

a)

Hematoxylin

b)

Eosin

c)

Giemsa

d)

Wright's stain

14.

At what magnifications should the slide be examined in the 'Thick Blood Smear' process?

a)

x10 and x40

b)

x50 and x200

c)

x100 and x400

d)

x500 and x1000

15.

What is the purpose of a 'Wet Blood Smear'?

a)

To identify blood protozoa and microfilaria

b)

To measure blood pressure

c)

To determine blood type

d)

To check for cholesterol levels

16.

What should be placed on the glass slide in a 'Wet Blood Smear'?

a)

A drop of water

b)

A drop of blood

c)

A drop of oil

d)

A drop of saline

17.

What is used to cover the blood drop in a 'Wet Blood Smear'?

a)

A glass slide

b)

A cover slip

c)

A piece of paper

d)

A plastic sheet

18.

At what magnifications should the slide be examined using a compound microscope?

a)

x50, x100, x150

b)

x100, x200, x400

c)

x200, x300, x500

d)

x400, x500, x600

19.

What is the main advantage of using a 'Thick Blood Smear' over a 'Thin Blood Smear'?

a)

It requires less blood

b)

It allows for better visualization of parasites

c)

It is quicker to prepare

d)

It is easier to stain

20.

Which of the following is a common mistake when preparing a blood smear?

a)

Using a dirty slide

b)

All of the above

c)

Not allowing the slide to dry

d)

Using too much blood

21.

What is the purpose of using a cover slip in a 'Wet Blood Smear'?

a)

To enhance the staining process

b)

To prevent evaporation of the sample

c)

To flatten the blood drop for better viewing

d)

To protect the sample from contamination