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Chapter 9: Manipulating DNA

Total questions: 25

Worksheet time: 27mins

Name
Class
Date
1.
This cuts DNA at specific sequences
a)
Plasmid
b)
Recombinant DNA
c)
Restriction enzyme
d)
Gel electrophoresis
2.
This diagram represents which of the following? 
a)
Gel electrophoresis
b)
Gene therapy
c)
Cloning 
d)
Genetic engineering
3.
The manipulation of DNA is called
a)
Genetic engineering
b)
Karyotype
c)
Plasmid
d)
Gel electrophoresis
4.
The process when an electrical current is used to separate a mixture of DNA is called
a)
Recombinant DNA
b)
Plasmid
c)
Gel electrophoresis
d)
Restriction enzyme
5.
Characteristics that are encoded in DNA and inherited from an organisms parents.
a)
genetic traits
b)
nuclues
c)
chromosomes
d)
cells
6.

How do restriction enzymes "know" where to cut DNA

a)

A specific sequence of nucleotides

b)

They cut randomly

c)

Distance from the centromere

7.

"Sticky-ends" are DNA strands with:

a)

single-stranded ends

b)

ends cut flat by an enzyme

c)

no way to be recombined

8.
Which is used to cut DNA molecules at specific points?
a)
gel electrophoresis
b)
PCR analysis
c)
a restriction enzyme
d)
recombinant DNA
9.
molecular scissors that "cut' DNA
a)
plasmid
b)
restriction enzymes
c)
recombinant DNA
d)
gene
10.
How do scientists cut DNA into smaller strands?
a)
restriction enzymes
b)
scissors
c)
ligase
d)
agarose
11.
GMO stands for
a)
Genetically Made Organism
b)
Growing More Organisms
c)
Genetically Modified Organism
d)
Growing Many Organisms
12.

A type of cell that can differentiate and become any type of cell that is needed in the body?

a)

Blood cells

b)

Muscle Cells

c)

Skin Cells

d)

Stem Cells

13.

What biotechnology is this a picture of?

a)

Gel Electrophoresis --> DNA fingerprinting

b)

Stem Cell Therapy

c)

Cloning

d)

GMO

14.
Which 2 molecules form the sides (backbone) of the DNA ladder?
a)
deoxyribose sugar and adenine
b)
deoxyribose sugar and a hydrogen bond
c)
deoxyribose sugar and the nucleus
d)
deoxyribose sugar and phosphate
15.
What will the matching sequence be on a strand that reads AAGCTAT?
a)
AACGATA
b)
TTCGTTA
c)
CCTGATA
d)
TTCGATA
16.
DNA codes for...
a)
bases
b)
proteins
c)
amino acids
d)
phosphates
17.
Which two scientists are accredited with correctly deducing the double helix model of DNA?
a)
Watson and Crick
b)
Wilkins and Franklin
c)
Wilkins and Crick
d)
Watson and Franklin
18.

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

a)

larger, and move slower

b)

larger, and move faster

c)

smaller, and move faster

d)

smaller, and move slower

19.

This technique used to analyze DNA involves the

a)

synthesis of new DNA strands from subunits

b)

separation of DNA fragments based on size

c)

production of genetically engineered DNA molecules

d)

removal of defective genes from DNA

20.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
21.
Which is the primary purpose of using restriction enzymes in gel elctrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
22.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
23.
Which suspect is linked to the crime scene by this DNA analysis
a)
Suspect A
b)
Suspect C
c)
Suspect D
d)
Suspect B
24.
Shannon does not know who her father is.  There are three possibilities:  Jack, Daniel and Bill.  Who is the father?
a)
Jack
b)
Daniel
c)
Bill
25.

What applications can agarose gel electrophoresis be used for?

a)

Parental Testing

b)

Criminal Investigations

c)

Sequence Endangered Species DNA

d)

All of the above

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