WorksheetsDNA Methylation or Histone Acetylation
Total questions: 11
Worksheet time: 7mins
There are 4definitions below, select the answer that correctly labels each definition
1. Pattern of inheritance where modifications occur to a nuclear gene or chromosome that alters gene expression
2. The combination of changes in gene expression within the genome
3. Chemically modified from the addition of a different onto the end of the tail
1.Epimutation, 2.Histone Modification, 3.Epigenome
1.CpG Island, 2.Histone Modifications, 3.Epigenome
1.Epigenetics, 2.Epigenome, 3. Histone Modification
What strategy of methylation detection, converts all cytosine's to uracil's and leaves 5-methycytosine residues unaffected?
Comparitive genomic hybridisation
Bisulfite conversion
Chromatin immuniprecipitation
Digestion of DNA by methylation-sensitive or insensitive restriciton endonuclease
Select the options that apply to a CpG island
Highly enriched with CpG sequences
at least 200 bp in length
GC content of less than 50 %
Observe-to-expect ratio of at least 60%
Types of epigenetic changes include (you can select more than one):
DNA Methylation
DNA Acetylation
Histone modifications
Non-coding RNA
All of that above
Commonly, _________ of histones leads to the silencing of genes.
phosphorylation
methylation
acetylation
All of these could silence the genes
Histones are proteins that bind to and order the DNA into tight clusters, making it inaccessible to transcription machinery. How does the cells loosen the interaction of the histone and DNA?
By adding acetyl groups to the DNA
By methylating the DNA
By adding acetyl groups to the histone
By removing acyl groups from the histone
A chemical modification of DNA that does not affect the nucleotide sequence of a gene but makes that gene less likely to be expressed.
acetylation
methylation
point mutation
in/dels
Which techniques among those below can detect the methylation of cytosine at the single-base resolution?
Bisulfite sequencing
MeDIP Sequencing
MBD Sequencing
ChIP-Seq
Which of the following is NOT an advantage of affinity enrichment methods for DNA methylation profiling
Cost-effective
Can differentiate between 5mC and 5hmC
No mutation introduced
Can detect at single-base resolution
Which of the following statements is correct (you can choose more than one)?
MeDIP-Seq works on double-stranded DNA, while MDB-Seq works on single-stranded DNA
MeDIP-Seq relies on the use of methylated cytosine antibodies, while MDB-Seq relies on methyl-domain binding proteins.
MBD-Seq is more sensitive to regions with higher CpG density, while MeDIP-Seq is more sensitive to regions with lower CpG density
MeDIP-Seq has different options for elution fractions so it can target regions with different CpG densities while MDB-Seq does not.
Which types of DNA-protein interactions below can be detected by XChip (you can choose more than one)?
H3K4me2
H3K27ac
transcription factor nuclear factor κB (NF-κB)
All of these above
