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WorksheetsDNA TCI
Total questions: 27
Worksheet time: 30mins
Name
Class
Date
1.
Separation of DNA using electric current is called.....?
a)
Gel Electrophoresis
b)
Chromosome painting
c)
polymerage chain reaction
d)
Restrictive Enzyme Sorting
2.
What is the enzymatic function of restriction enzymes?
a)
To add new nucleotides to the growing strand of DNA.
b)
To join nucleotides during replication.
c)
To join nucleotides during transcription.
d)
To cleave nucleic acids at specific sites.
3.
The unpaired nucleotides produced by the action of restriction enzymes are referred to as
a)
sticky end.
b)
base sequence.
c)
single strands.
d)
restriction fragments.
4.
In order to insert a human gene into plasmid, both must
a)
code for the same gene product.
b)
be cut by the same restriction enzyme.
c)
originate from the same type of cell.
d)
have identical sequences.
5.
What enzyme forms covalent bonds between restriction fragments?
a)
DNA primase
b)
DNA helicase
c)
DNA ligase
d)
DNA polymerase
6.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA
a)
I, II, IV, III, V
b)
III, II, IV, V, I
c)
II, III, V, IV, I
d)
III, IV, V, I, II
7.
Organisms that have DNA from a different species artificially inserted into their cells are called
a)
bioremediated
b)
transgenic
c)
cultured
d)
a clone
8.
GMO stands for
a)
Genetically Made Organism
b)
Growing More Organisms
c)
Genetically Modified Organism
d)
Growing Many Organisms
9.
All of the following are advantages of GMO's except
a)
pest resistant crops
b)
more nutritional foods
c)
unknown long term health effects
d)
creating more human insulin
10.
You have benefited from selective breeding by having more agricultural produce than would have been possible otherwise.
a)
True
b)
False
11.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
12.
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
a)
using recombinant DNA to produce human hormones from bacteria
b)
testing parents for genetic disorders before they have children
c)
engineering fruits and vegetables that resist insects and other pests
d)
C. developing ways to identify criminals through DNA fingerprinting
13.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
14.
Combining DNA from 2 different organisms is called
a)
Bioengineering
b)
Biotechnology
c)
Recombinant DNA
d)
Genetic Engineering
15.
This picture is an example of what type of DNA cut?
a)
Sticky Ends
b)
Blunt Ends
16.
To amplify (create more) DNA, which process is used?
a)
Gel Electrophorisis
b)
Cloning
c)
Polymerase Chain Reaction (PCR)
d)
Transgenic Process
17.
DNA fingerprinting works because
a)
Most genes are dominant
b)
Most people have DNA that contains repeats
c)
The most important genes are different among most people
d)
No two people, except identical twins, have exactly the same DNA
18.
A new variety of rice called golden rice has been genetically modified to have vitamin A. people in starving countries can eat this rice and receive vitamin A. Without this rice people go blind from vitamin A deficiency. What word(s) below best describes this rice?
a)
Electrophoresis
b)
DNA fingerprint
c)
Genetically modified organism
d)
Clone
19.
In gel electrophoresis, smaller molecules move . . .
a)
really slow
b)
really fast
c)
to the negative side
d)
to the positive side
20.
Scientists uncover the remains of a rodent-like creature. They compare its skeleton to various rodents, but need further proof that it is a part of the rodent family. What other evidence would they most likely rely upon?
a)
DNA comparision
b)
a karyotype
c)
diet comparison
d)
the flora and fauna of its habitat
21.
This picture shows how what is made?
a)
Recombinant DNA
b)
PCR
c)
Gel Electrophorisis
d)
Clones
22.
What applications can gel electrophoresis be used for?
a)
Parental Testing
b)
Criminal Investigations
c)
Sequence Endangered Species DNA
d)
All of the above
23.
Which two bear species are most closely related?
a)
Bear 1 and Bear 2
b)
Bear 1 and Bear 3
c)
Bear 2 and Bear 3
d)
There’s no way to tell
24.
In which step would the enzyme ligase be used?
a)
A
b)
B
c)
C
d)
D
25.
Which of these is the "gene of interest"?
a)
A
b)
B
c)
C
d)
D
26.
What would have caused the plasmid (B) to open?
a)
restriction enzyme
b)
ligase
c)
gene therapy
d)
sticky ends
27.
In step D, recombinant DNA is being placed back into _____ to be cloned by binary fission.
a)
bacteria
b)
a human
c)
a virus
d)
a sheep
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