WorksheetsCrispr
Total questions: 25
Worksheet time: 25mins
How does the guide RNA help the cell know where to cut the DNA?
Guide RNA helps to find the PAM
Guide RNA is complementary to the DNA sequence
Guide RNA always binds to the same sequence of DNA
Like restriction sites, they are always palindromes
The enzyme portion of CRISPR??- this process is called ________.
or What enzyme is used in CRISPR gene editing?
CRISPR
Replicase
Lysozyme
Cas9
What does the cas9 protein do?
cut viruses
Cut RNA
cut DNA
ingest bacteria
What does the guide RNA do?
destroy invaders like bacteriophages immediately
cut invading pieces of RNA
recognize invaders
recognize sequences of DNA to be edited.
What is the role of gRNA? (gRNA -> guide RNA)
the cell recognizes that DNA is damaged and tries to repair it
ensures that the Cas9 enzyme cuts at the right point in the genome
use the DNA repair machinery to introduce changes to one or more genes
makes a cut across both strands of the DNA
What does CRISPR stand for?
Clustered regularly interactive short palindromic repeats
Clustered regularly interspaced short palindromic repeats
Deoxyribonucleic acid
Regularly interspaced short palindromic repeats
CRISPR-Cas9 was discovered in which of the following organisms?
protists
fungi
bacteria
viruses
What did scientists look at to help them create CRISPR?
Sheep cloning
Palindromic repeats
Common Cold
Bacteria fighting off viruses
What is one way CRISPR has already been used to change genes?
Clone sheep
Give people superpowers
Cure animal diseases
Military purposes
How does CRISPR differ from previous techniques of gene editing?
slower, more expensive, but more accurate
faster, cheaper, and more accurate
faster, more expensive, more accurate
slower, cheaper, less accurate
What is the definition of genetic engineering?
Studying the DNA of plants and animals
Making changes in the DNA code of living organisms
The discovery of DNA Using DNA to reproduce organisms
Using DNA to reproduce organisms
Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
What does CRISPR stand for?
Clustered regularly interactive short palindromic repeats
Clustered regularly interspaced short palindromic repeats
Deoxyribonucleic acid
Regularly interspaced short palindromic repeats
How does the guide RNA help the cell know where to cut the DNA?
Guide RNA helps to find the PAM
Guide RNA is complementary to the DNA sequence
Guide RNA always binds to the same sequence of DNA
Like restriction sites, they are always palindromes
The enzyme portion of CRISPR??- this process is called ________.
CRISPR
Replicase
Lysozyme
Cas9
CRISPR can edit DNA. What organisms naturally has CRISPR?
Viruses
Humans
Plants
Bacteria
Scientists have adapted CRISPR-Cas9 to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
Which of the following examples of genetic engineering repairs existing abnormal genes?
CRISPR
PCR
Gel Electrophoresis
Cloning
What do we call the science of using the genetic makeup living things to create products?
Biotechnology
Genealogy
Pharmacology
Neurology
What is the primary function of the CRISPR-Cas9 system in bacteria?
A) To cut invading viral DNA
B) To replicate bacterial DNA
C) To synthesize proteins
D) To repair bacterial cell walls
Which component of the CRISPR-Cas9 system is responsible for recognizing the target DNA sequence?
A) Cas9 enzyme
B) Guide RNA (gRNA)
C) PAM sequence
D) DNA polymerase
In the context of CRISPR, what does the term 'PAM' stand for?
A) Protospacer Adjacent Motif
B) Protein Associated Marker
C) Palindromic Associated Motif
D) Phage Associated Marker
How does CRISPR-Cas9 achieve specificity in gene editing?
A) By using a specific DNA polymerase
B) Through the complementary base pairing of guide RNA
C) By cutting at random sites
D) By using a universal cutting enzyme
What is a potential application of CRISPR technology in medicine?
A) Editing genes to cure genetic disorders
B) Cloning human organs
C) Synthesizing new proteins
D) Creating new species
Which of the following best describes the CRISPR-Cas9 mechanism?
A) A method for DNA replication
B) A tool for targeted DNA editing
C) A process for protein synthesis
D) A technique for RNA transcription
