WorksheetsChapter 12 KUV
Total questions: 21
Worksheet time: 14mins
Recombinant DNA technology is used:
For production of herbicide-resistant plant.
To make a particular protein.
To clone a specific piece of DNA
All of the above.
Which of the following is correct about host cell?
Plasmid is an example of host cell.
Unable to receive DNA molecule through transformation.
An organism that prevents cloning vectors from multiplying.
An organism that is used to receive recombinant DNA for cloning purposes.
What is the purpose of amplification?
To screen for recombinant bacteria.
To join the rDNA with the host cell.
To produce multiple copies of the desired gene.
To prevent the bacteria from reproducing.
Which of these pairs is incorrectly matched?
DNA ligase – mapping human chromosomes
Target DNA – gene of interest
Taq polymerase – PCR
Plasmid as a vector – isolated from bacteria
Which of these might be a biotechnology product?
Vaccine.
Modified enzyme.
Protein hormone.
All of the above.
Plants are more readily manipulated by genetic engineering than are animals because:
Plant genes do not contain introns.
A somatic plant cell can often give rise to a complete plant.
Genes can be inserted into plant cells by microinjection.
Plant cells have larger nuclei.
The following are steps involved in gene cloning procedures. Rearrange them into the correct sequence:
I. Transformation and amplification.
II. Screen for bacteria that contain recombinant
DNA.
III. Insert DNA fragment into the cut bacterial
plasmid.
IV. Cleave the plasmid and human DNA with the same restriction enzyme.
V. Isolate the bacterial plasmid and human DNA.
A. I, II, III, IV, V
B. V, IV, III, I, II
C. V, IV, III, II, I
D. IV, V, III, I, II
In genetic engineering, DNA ligase is used as:
a probe
a sealing enzyme
a restriction enzyme
a mutagen
Which one of the following statements about Restriction Endonuclease is true
all restriction endonucleases cut DNA at specific sites
all restriction endonucleases cut DNA at random sites
all restriction endonucleases join DNA segments at specific sites
all restriction endonucleases join DNA at random sites
The first step in PCR is
Primer annealing
Denaturation
Primer extension
None of these
What is the denaturation step?
mutating RNA polymerase
Cooling DNA to allow primers to form
separating DNA helix in a cold environment
separating DNA helix with using heat
CRISPR system evolved in bacteria and archaea in their defense against....
Coronaviruses
Chemical reagents
Bacteriophage
Other bacteria
Which suspect should be charged with the crime?
Suspect 1
Suspect 2
Suspect 3
None of these suspects
Which sequence of DNA bases would pair with this partial strand
ATG TGA CAG
ATG TGA CAG
TAC ACT GTC
GTA AGT GAC
CAT TCA CTG
This process of DNA fingerprinting separates the DNA fragments through a gel using an electrical current.
DNA Extraction
Polymerase Chain Reaction
Gel Electrophoresis
DNA Replication
Which suspect is linked to the crime scene by this DNA analysis?
Suspect A
Suspect C
Suspect D
Suspect B
In Paternity Tests, to know Whether a person wants to prove they are or are not the father, it is necessary:
DNA from the baby solely
DNA from both the potential father and the potential mother
DNA from both the potential father and the baby
