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Identification of Gram-negative Bacteria

Total questions: 20

Worksheet time: 10mins

Name
Class
Date
1.

Shigella sp. has rod shape.

a)

TRUE

b)

FALSE

2.

Salmonella sp. has rod shape.

a)

TRUE

b)

FALSE

3.

Neisseria sp. has cocci shape.

a)

TRUE

b)

FALSE

4.

Haemophilus sp. has coccobacilli shape.

a)

TRUE

b)

FALSE

5.

E. coli can grow on blood agar and MacConkey.

a)

TRUE

b)

FALSE

6.

Pseudomonas sp. is non-lactose fermenter.

a)

TRUE

b)

FALSE

7.

Report this TSI result.

a)

A/A with H2S

b)

AG/A with H2S

8.

Report this TSI result.

a)

AG/A

b)

AG/K

c)

K/K

d)

AG/A

9.

Identify bacteria that could utilize only 1 sugar in the TSI test.

a)

b)

c)

10.

Identify the urease test.

a)

Positive result (pink)

b)

11.

Identify the bacteria species.

a)

E. coli

b)

Klebsiella sp.

c)

Salmonella sp.

d)

Pseudomonas sp.

12.

Below are the X and V factors test. Identify result for Haemophilus influenza.

a)

b)

c)

13.

Neisseria sp. is a Gram-negative bacteria and can grow on MacConkey.

a)

TRUE

b)

FALSE

14.

Haemophilus sp. is a Gram-negative bacteria and can grow on MacConkey.

a)

TRUE

b)

FALSE

15.

Identify shape of Neisseria sp.

a)

Cocci

b)

Rod

c)

Comma

d)

Spira;

16.

Satellite test is perform to confirm ________.

a)

Pseudomonas aeruginosa

b)

Neisseria meningitidis

c)

Haemophilus influenza

d)

E. coli

17.

Name a culture media used in satellite test.

a)

Nutrient agar

b)

TSA agar

c)

Blood agar

d)

Chocolate agar

18.

Can hemolysis be determined using chocolate agar?

a)

YES

b)

NO

19.

In Gram-negative bacteria group, only Pseudomonas sp. shows positive oxidase.

a)

TRUE

b)

FALSE

20.

Reagent used in Indole test is __________.

a)

Methyl Red

b)

H2S

c)

Kovac's

d)

Phenol red