WorksheetsBACE: Biotechnology Skills
Total questions: 14
Worksheet time: 7mins
6 How do you identify and properly handle reagents?
Read labels carefully, store them correctly, and use protective equipment if needed.
Only read labels and ignore storage instructions.
Use any equipment available without checking for protection.
Store reagents in any container without labeling.
12 How do you properly use and maintain a biosafety cabinet (BSC)?
Keep the airflow running, use gloves, clean the surfaces, and avoid blocking vents.
Only use gloves when handling hazardous materials.
Clean the surfaces once a week regardless of usage.
Block the vents to maintain a stable airflow.
4 How do you measure and adjust the pH of a solution with a pH meter?
Use a pH meter to check how acidic or basic a solution is, then add acid or base to adjust it.
Measure the temperature of the solution and add water to adjust the pH.
Use litmus paper to determine the pH and then add salt to adjust it.
Boil the solution and measure the steam to determine the pH.
7 What is aseptic or sterile technique?
Aseptic technique means working in a way that prevents contamination by bacteria or other germs.
Aseptic technique involves using high temperatures to sterilize equipment.
Aseptic technique is a method of cleaning surfaces with disinfectants.
Aseptic technique refers to the process of washing hands before surgery.
1 How do you accurately measure liquids using micropipettes and serological pipets?
Use a micropipette for very small liquid volumes and a serological pipet for larger amounts. Always set the correct volume, hold the pipette at the right angle, and release liquid smoothly.
Use a serological pipet for all liquid volumes regardless of size.
Always use a micropipette for all measurements to ensure accuracy.
Measure liquids by eye without any tools for quick results.
10 How do you properly use a spectrophotometer?
Place a sample in the machine, select the right settings, and measure how much light passes through the liquid.
Add the sample to a beaker and stir it before measuring.
Calibrate the machine with a blank sample and then measure the absorbance directly.
Use the spectrophotometer only for solid samples, not liquids.
14 How does automation impact the biotechnology industry?
Automation makes experiments faster, more accurate, and safer by using machines instead of people for repetitive tasks.
Automation reduces the need for skilled labor in biotechnology.
Automation eliminates the need for any human oversight in experiments.
Automation increases the cost of experiments due to machinery maintenance.
2 How do you accurately measure mass using an electronic balance?
Turn on the balance, make sure it reads zero, place the object or container on the scale, and record the weight.
Place the object on the balance without turning it on, then record the weight.
Add a known weight to the balance and adjust until it reads zero before measuring the object.
Use a ruler to measure the dimensions of the object and calculate the mass based on volume.
8 How do you properly culture microorganisms?
Provide them with food, warmth, and the right environment to grow in a lab.
Only provide them with food and water.
Keep them in a cold environment to slow down growth.
Isolate them from all other organisms.
11 Why are calibration and validation important in experiments?
Calibration ensures instruments give correct measurements, and validation makes sure results are reliable.
Calibration is only necessary for electronic instruments, while validation is optional.
Calibration and validation are only important in theoretical experiments, not practical ones.
Calibration is about adjusting instruments, while validation is about increasing experiment duration.
5 How do you properly perform a serial dilution?
Mix a known amount of liquid with a new solution multiple times to gradually reduce its concentration.
Dilute the solution only once to achieve the desired concentration.
Add a large volume of solvent to the solution without mixing.
Use a centrifuge to separate the components before dilution.
3 How do you properly prepare solutions and buffers?
Measure the correct amounts of chemicals and mix them in water while following a recipe. Use a pH meter to check the acidity or basicity if needed.
Mix random amounts of chemicals without measuring them.
Use only distilled water without any chemicals.
Prepare solutions and buffers by heating them to high temperatures.
9 How do you properly use a centrifuge?
Balance the tubes, close the lid, set the speed, and turn it on to spin samples at high speed.
Place the samples in the centrifuge without balancing them, close the lid, and turn it on.
Set the speed first, then balance the tubes, and finally close the lid before turning it on.
Only close the lid and turn it on without any other preparations.
13 How do you properly use electrophoresis equipment?
Prepare a gel, load samples, apply electricity, and watch DNA or proteins move through the gel.
Mix samples with a buffer and freeze them before loading.
Use a centrifuge to separate the samples before applying electricity.
Heat the gel to 100 degrees Celsius before loading the samples.
