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WorksheetsSijil Professional CRISPR
Total questions: 15
Worksheet time: 6mins
What is the primary function of the Cas9 protein in the CRISPR system?
Acts as a guide RNA
Cuts RNA molecules
Binds to PAM sequences only
Cleaves double-stranded DNA at specific target sites
In CRISPR-Cas9 genome editing, the specificity of the DNA target site is determined by:
The Cas9 enzyme sequence
The DNA polymerase activity
The guide RNA sequence
The PAM sequence alone
Which of the following is a requirement for Cas9 to initiate DNA cleavage?
A DNA template strand
High ATP concentration
Presence of a Protospacer Adjacent Motif (PAM)
RNA polymerase binding
What is the typical PAM sequence recognized by Streptococcus pyogenes Cas9?
AATAAA
TATA box
NGG
TTAA
One limitation of CRISPR-Cas9 technology is the potential for:
Efficient transduction
Accurate genome replication
Off-target effects
Increased recombination frequency
Which statement best describes homology-directed repair (HDR)?
It is the default repair mechanism after Cas9-induced breaks.
It requires a homologous DNA template to introduce precise edits.
It functions only in prokaryotes.
It randomly inserts nucleotides at the break site.
Which of the following tools allows single-base editing without introducing double-stranded breaks?
TALEN
Base editors (e.g., cytidine deaminase fused to dCas9)
Standard CRISPR-Cas9
Zinc finger nucleases
Prime editing differs from base editing in that it:
Lacks any Cas9 component
Requires an RNA-directed DNA polymerase but no guide RNA
Can introduce insertions, deletions, and all base conversions
Can only perform A-to-G or C-to-T edits
Which CRISPR system is most suitable for targeting and degrading RNA rather than DNA?
Cas9
Cas13
Cas12a
dCas9
In CRISPR applications for cancer immunotherapy, what is the main advantage of using CRISPR to engineer T cells?
T cells become non-functional
T cells can be redirected to target tumor-specific antigens
T cells lose all receptors and become universal
CRISPR prevents immune activation entirely
In CRISPR-based cancer immunotherapy, which of the following strategies enhances T-cell functionality and persistence?
Silencing PD-1 using RNA interference
CRISPR-mediated knockout of immune checkpoint genes like PDCD1
Inserting tumor-specific antigens into NK cells via retroviral vectors
Enhancing apoptosis through CRISPR-edited caspase genes
What is the main rationale for using CRISPR-Cas13 in antiviral therapy?
Cas13 integrates antiviral DNA into host genomes
Cas13 targets and degrades viral RNA in a sequence-specific manner
Cas13 induces host immune response via dsDNA breaks
Cas13 creates heritable resistance by editing germline DNA
What is the primary advantage of using CRISPR technology in gene therapy?
It can only target specific genes
It is less expensive than traditional methods
It does not require a delivery system
It allows for precise modifications to the genome
Which of the following best describes the role of guide RNA in the CRISPR-Cas9 system?
It cleaves the target DNA directly
It enhances the binding of Cas9 to the PAM sequence
It directs the Cas9 enzyme to the target DNA sequence
It provides a template for DNA repair
In the context of CRISPR technology, what does the term 'off-target effects' refer to?
Enhanced specificity of the guide RNA
Unintended modifications at non-target sites in the genome
Increased efficiency of gene editing
Successful edits that are not desired
