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MIDTERM Glycolysis

Total questions: 20

Worksheet time: 10mins

Name
Class
Date
1.

Which of the following is the first step of glycolysis?

a)

Fructose-6-phosphate → Fructose-1,6-bisphosphate

b)

Glucose → Glucose-6-phosphate

c)

Glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate

d)

Phosphoenolpyruvate → Pyruvate

2.

What is the primary function of glycolysis?

a)

To produce oxygen

b)

To break down glucose into pyruvate, generating ATP

c)

To convert pyruvate into glucose

d)

To synthesize fatty acids

3.

Which enzyme catalyzes the rate-limiting step of glycolysis?

a)

Hexokinase

b)

Phosphofructokinase-1 (PFK-1)

c)

Pyruvate kinase

d)

Aldolase

4.

In the glycolytic pathway, NAD+ is reduced to which molecule?

a)

ATP

b)

NADH

c)

FADH2

d)

Pyruvate

5.

How many ATP molecules are consumed during the investment phase of glycolysis?

a)

2 ATP

b)

4 ATP

c)

1 ATP

d)

3 ATP

6.

Which of the following molecules is produced in the energy generation phase of glycolysis?

a)

NADH

b)

ATP

c)

Pyruvate

d)

All of the above

7.

At which step of glycolysis is ATP generated through substrate-level phosphorylation?

a)

Step 1

b)

Step 4

c)

Step 7

d)

Step 10

8.

Which of the following enzymes is activated by fructose-2,6-bisphosphate in glycolysis?

a)

Hexokinase

b)

Phosphofructokinase-1 (PFK-1)

c)

Glyceraldehyde-3-phosphate dehydrogenase

d)

Pyruvate kinase

9.

What is the final product of glycolysis?

a)

Glucose

b)

Oxygen

c)

Pyruvate

d)

Lactate

10.

Which of the following molecules inhibits phosphofructokinase-1 (PFK-1)?

a)

AMP

b)

ATP

c)

Fructose-2,6-bisphosphate

d)

NADH

11.

Which enzyme catalyzes the conversion of phosphoenolpyruvate to pyruvate?

a)

Hexokinase

b)

Pyruvate kinase

c)

Phosphoglycerate kinase

d)

Aldolase

12.

In glycolysis, how many molecules of ATP are produced for each molecule of glucose?

a)

1 ATP

b)

2 ATP

c)

3 ATP

d)

4 ATP

13.

Which of the following molecules is NOT a product of glycolysis?

a)

ATP

b)

NADH

c)

Pyruvate

d)

Oxygen

14.

Which molecule can regulate pyruvate kinase activity?

a)

AMP

b)

ATP

c)

Acetyl-CoA

d)

Fructose-1,6-bisphosphate

15.

The enzyme aldolase in glycolysis catalyzes the cleavage of which molecule?

a)

Fructose-6-phosphate

b)

Fructose-1,6-bisphosphate

c)

Glucose

d)

Glyceraldehyde-3-phosphate

16.

Which phase of glycolysis consumes ATP?

a)

Energy generation phase

b)

Energy investment phase

c)

Both phases

d)

Neither phase

17.

What is the role of NADH in glycolysis?

a)

To generate ATP

b)

To oxidize glucose

c)

To reduce NAD+ to NADH

d)

To transport electrons to the electron transport chain

18.

Which of the following molecules activates phosphofructokinase-1 (PFK-1)?

a)

Citrate

b)

ATP

c)

AMP

d)

Glucose

19.

During anaerobic conditions, what happens to pyruvate produced in glycolysis?

a)

It is converted to acetyl-CoA

b)

It is converted to lactate (in muscles) or ethanol (in yeast)

c)

It enters the citric acid cycle

d)

It is stored as glycogen

20.

Which molecule is produced by the conversion of G3P to 1,3-bisphosphoglycerate in glycolysis?

a)

ATP

b)

NADH

c)

Pyruvate

d)

Fructose-1,6-bisphosphate

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